Epidemiological investigation of equine piroplasmosis in China by enzyme-linked immunosorbent assays.
Abstract: The objective of this study is to investigate the seroprevalence of equine piroplasmosis in China. A total of 1990 sera were collected from clinically healthy horses in various districts located in ten different provinces of China and examined by using indirect enzyme-linked immunosorbent assays (ELISAs) with recombinant Theileria equi (T. equi) merozoite antigen 2 (rEMA-2) and Babesia caballi (B. caballi) 48-kDa rhoptry protein (rBc48), respectively. The results showed that 1,018 (51.16%) and 229 (11.51%) samples were positive for B. caballi and T. equi infection, respectively. The number of samples with mixed infection was 152 (7.64%). These results indicated that equine piroplasmosis was widespread in China.
Publication Date: 2013-11-29 PubMed ID: 24292247PubMed Central: PMC4064140DOI: 10.1292/jvms.13-0477Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Diagnostic Technique
- Disease control
- Disease Management
- Disease Outbreaks
- Disease Prevalence
- Disease Surveillance
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Diseases
- Equine Health
- Horses
- Infection
- Infectious Disease
- Piroplasmosis
- Public Health
- Serological Surveys
- Seroprevalence
- Theileria equi
- Veterinary Medicine
- Veterinary Research
Summary
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This research study aims to understand the prevalence of a horse disease called equine piroplasmosis in China, using a blood-testing method known as an enzyme-linked immunosorbent assay.
Background and Aim
- The study centers around equine piroplasmosis, a disease that affects horses and is caused by two parasitic organisms: Theileria equi and Babesia caballi.
- The disease is transmitted via ticks and can cause severe symptoms in horses including fever, anemia, jaundice, and in severe cases, death.
- Understanding the prevalence of this disease can enable better prevention and treatment strategies.
- The researchers aimed to use an enzyme-linked immunosorbent assay (ELISA) to investigate the frequency of this disease in horses in China.
Methodology
- The researchers collected serum, or blood samples, from 1990 healthy horses from across ten different provinces in China.
- These samples were tested using an indirect enzyme-linked immunosorbent assay (ELISA), a commonly used laboratory test that uses enzymes and color changes to identify the presence of certain substances, in this case, the two parasites causing equine piroplasmosis.
- Specific antigens associated with the parasites (T. equi merozoite antigen 2 and B. caballi 48-kDa rhoptry protein) were used in the ELISA.
Results
- Of the tested samples, 51.16% were found to be positive for B. caballi, and 11.51% for T. equi. This is a substantial proportion, indicating a high prevalence of equine piroplasmosis in Chinese horses.
- In addition, 7.64% of samples reflected a mixed infection, with horses being affected by both parasites simultaneously.
- This demonstrates that equine piroplasmosis is widespread in China, highlighting the need for improved strategies for prevention and treatment.
Implications
- This research has crucial implications for veterinary medical practices in China and could inform similar studies in other parts of the world.
- By understanding the prevalence of equine piroplasmosis, the researchers have drawn attention to the significance of this disease within China’s equine population.
- This could potentially lead to stricter control measures, improved diagnostic procedures, and more effective treatments for horses affected by T. equi and B. caballi.
Cite This Article
APA
Wang M, Guo W, Igarashi I, Xuan X, Wang X, Xiang W, Jia H.
(2013).
Epidemiological investigation of equine piroplasmosis in China by enzyme-linked immunosorbent assays.
J Vet Med Sci, 76(4), 549-552.
https://doi.org/10.1292/jvms.13-0477 Publication
Researcher Affiliations
- Harbin Veterinary Research Institute-Michigan State University Joint Laboratory of Innate Immunity & State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Maduan Street 427, Nangang District, Harbin 150001, P. R. China.
MeSH Terms
- Animals
- Antigens, Protozoan / genetics
- Antigens, Protozoan / immunology
- Babesia / genetics
- Babesia / immunology
- Babesiosis / epidemiology
- China / epidemiology
- Enzyme-Linked Immunosorbent Assay / veterinary
- Horse Diseases / epidemiology
- Horse Diseases / parasitology
- Horses
- Seroepidemiologic Studies
- Theileria / genetics
- Theileria / immunology
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Citations
This article has been cited 6 times.- Yang G, Chen K, Guo W, Hu Z, Qi T, Liu D, Wang Y, Du C, Wang X. Development of a Test Card Based on Colloidal Gold Immunochromatographic Strips for Rapid Detection of Antibodies against Theileria equi and Babesia caballi. Microbiol Spectr 2022 Feb 23;10(1):e0241121.
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