Equine adipose-tissue derived mesenchymal stem cells and platelet concentrates: their association in vitro and in vivo.
Abstract: Equine mesenchymal stem cells (MSC) are of particular interest both for basic research and for the therapeutic approach to musculoskeletal diseases in the horse. Their multilineage differentiation potential gives them the capability to contribute to the repair of tendon, ligament and bone damage. MSCs are also considered a promising therapeutic aid in allogeneic cell transplantation, since they show low immunogenicity and immunomodulating functions.Adipose tissue-derived adult equine stem cells (AdMSC) can be isolated, expanded in vitro and then inoculated into the damaged tissue, eventually in the presence of a biological scaffold. Here we report our preliminary experience with adipose-derived mesenchymal stem cells in allogeneic cell-therapy of tendonitis in the horse. MSCs, derived from visceral adipose tissue, were grown in the presence of autologous platelet lysate and characterized for their differentiation and growth potential. Expanded AdMSC were inoculated into the damaged tendon after their dispersion in activated platelet-rich plasma (PRP), a biological scaffold that plays an important role in maintaining cells in defect sites and contributes to tissue healing. Fourteen out of sixteen treated horses showed a functional recovery and were able to return to their normal activity.
Publication Date: 2008-08-07 PubMed ID: 18683070DOI: 10.1007/s11259-008-9093-3Google Scholar: Lookup
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- Journal Article
Summary
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This study investigates the potential of using fat-derived stem cells and platelet concentrates in the treatment of tendonitis in horses. The researchers cultivated these stem cells with a platelet solution, then introduced this mixture into the affected area. The promising results suggest this approach could offer a functional recovery for horses with tendonitis.
Context and Objectives
- The researchers are interested in equine mesenchymal stem cells (MSC). These cells are versatile, able to transform into various other cells types. They are of immense interest for treating musculoskeletal diseases in horses, given their potential to fix damage to tendons, ligaments, and bone.
- Adipose tissue-derived mesenchymal stem cells (AdMSC) are stem cells derived from fat tissue. They are known for their low immune response, meaning they can be transplanted to other individuals without triggering a strong immune reaction.
- The aim of this study was to examine the use of adipose-derived MSCs in the treatment of tendonitis in horses, by transmuting these MSCs in autologous platelet lysate. This solution helps to stimulate cell growth and differentiation.
Methodology
- The MSCs were harvested from horse visceral fat tissue, expanded in autologous platelet lysate, and characterized based on their growth and differentiation potential.
- The expanded AdMSCs were then injected into the tendon suffering from tendonitis.
- To enhance the placement and function of these stem cells, they were dispersed in activated platelet-rich plasma (PRP). PRP serves as a biological scaffold, guiding and supporting cell growth in specific locations.
Results and Conclusion
- The results were promising, with 14 out of 16 treated horses showing functional recovery and returning to their normal activity.
- These findings present adipose-derived MSCs and PRP as a potential therapeutic solution for tendonitis in horses.
- Further research is needed to fully understand and optimize this potential treatment pathway.
Cite This Article
APA
Del Bue M, Riccò S, Ramoni R, Conti V, Gnudi G, Grolli S.
(2008).
Equine adipose-tissue derived mesenchymal stem cells and platelet concentrates: their association in vitro and in vivo.
Vet Res Commun, 32 Suppl 1, S51-S55.
https://doi.org/10.1007/s11259-008-9093-3 Publication
Researcher Affiliations
- Department of Animal Health, Veterinary Biotechnology, Food Quality and Safety, University of Parma, via del Taglio 8, Parma 43100, Italy.
MeSH Terms
- Adipose Tissue / cytology
- Adipose Tissue / physiology
- Animals
- Cell Transplantation / methods
- Cell Transplantation / veterinary
- Horse Diseases / surgery
- Horses / blood
- Horses / physiology
- Mesenchymal Stem Cell Transplantation / methods
- Mesenchymal Stem Cell Transplantation / veterinary
- Mesenchymal Stem Cells / cytology
- Mesenchymal Stem Cells / physiology
- Platelet Count
- Platelet-Rich Plasma
- Tendinopathy / surgery
- Tendinopathy / veterinary
- Tissue Engineering / methods
- Tissue Engineering / veterinary
- Transplantation, Homologous
- Treatment Outcome
References
This article includes 9 references
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- J Bone Joint Surg Am. 2007 Jan;89(1):74-81
- Blood. 2007 Nov 15;110(10):3499-506
- Vet Res Commun. 2007 Aug;31 Suppl 1:289-92
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