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BMC physiology2008; 8; 1; doi: 10.1186/1472-6793-8-1

Equine CTNNB1 and PECAM1 nucleotide structure and expression analyses in an experimental model of normal and pathological wound repair.

Abstract: Wound healing in horses is fraught with complications. Specifically, wounds on horse limbs often develop exuberant granulation tissue which behaves clinically like a benign tumor and resembles the human keloid in that the evolving scar is trapped in the proliferative phase of repair, leading to fibrosis. Clues gained from the study of over-scarring in horses should eventually lead to new insights into how to prevent unwanted scar formation in humans. cDNA fragments corresponding to CTNNB1 (coding for beta-catenin) and PECAM1, genes potentially contributing to the proliferative phase of repair, were previously identified in a mRNA expression study as being up-regulated in 7 day wound biopsies from horses. The aim of the present study was to clone full-length equine CTNNB1 and PECAM1 cDNAs and to study the spatio-temporal expression of mRNAs and corresponding proteins during repair of body and limb wounds in a horse model. Results: The temporal pattern of the two genes was similar; except for CTNNB1 in limb wounds, wounding caused up-regulation of mRNA which did not return to baseline by the end of the study. Relative over-expression of both CTNNB1 and PECAM1 mRNA was noted in body wounds compared to limb wounds. Immunostaining for both beta-catenin and PECAM1 was principally observed in endothelial cells and fibroblasts and was especially pronounced in wounds having developed exuberant granulation tissue. Conclusions: This study is the first to characterize equine cDNA for CTNNB1 and PECAM1 and to document that these genes are expressed during wound repair in horses. It appears that beta-catenin may be regulated in a post-transcriptional manner while PECAM1 might help thoracic wounds mount an efficient inflammatory response in contrast to what is observed in limb wounds. Furthermore, data from this study suggest that beta-catenin and PECAM1 might interact to modulate endothelial cell and fibroblast proliferation during wound repair in the horse.
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Publication Date: 2008-01-31 PubMed ID: 18237399PubMed Central: PMC2268708DOI: 10.1186/1472-6793-8-1Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the role of two specific genes, CTNNB1 and PECAM1, in wound healing processes in horses. It aims to understand why wounds on horse limbs often generate an excessive growth of granulation tissue, similar to benign tumors or human keloids, by studying the expressions of these genes during the repair of body and limb wounds using a horse model.

Background

  • Cuts or wounds on horses, particularly on their limbs, often lead to excessive growth of granulation tissue. This condition is clinically similar to benign tumors and resembles human keloids in which the scar continually proliferates leading to fibrosis.
  • This overgrowth of scar tissue, or scarring, can cause significant problems, and understanding its mechanisms in horses could help in preventing unnecessary scarring in humans.
  • The genes CTNNB1 and PECAM1, which code for beta-catenin and Platelet Endothelial Cell Adhesion Molecule-1 respectively, have been identified as potential contributors to this excessive proliferation during wound healing.

Study Objectives

  • The goal of the research was to clone the full-length versions of the equine CTNNB1 and PECAM1 genes and to observe their levels of expression during body and limb wound healing in horses.
  • The researchers aimed to identify a potential correlation between the activity of these genes and the development of excessive granulation tissue.

Methodology and Results

  • The study mainly involved the cloning of full-length cDNA corresponding to CTNNB1 and PECAM1 genes from horses.
  • Researchers observed that the CTNNB1 and PECAM1 genes both had up-regulated mRNA levels in wound healing, but did not return to their normal levels by the end of the study, indicating they contribute significantly to the wound healing process.
  • The expression of these genes was higher in body wounds than limb wounds, suggesting they might be more effective in promoting wound healing in body tissues. This could explain why limb wounds often develop complications like excessive granulation tissue.
  • The proteins for both genes, beta-catenin and PECAM1, were observed mainly in endothelial cells and fibroblasts, the areas where excessive granulation tissue often develops.

Conclusions and Implications

  • The results of the study suggest that beta-catenin (CTNNB1) may be regulated in a post-transcriptional manner, while PECAM1 could possibly help wounds on the horse’s body mount an efficient inflammatory response, a crucial part of wound healing.
  • These findings indicate that the CTNNB1 and PECAM1 genes play a key role in wound healing, with their interactions potentially influencing the proliferation of endothelial cells and fibroblasts during wound repair.
  • While the study sheds some light on the mechanisms of wound healing, further research is needed, especially in understanding how these mechanisms vary between wounds in different parts of the body, and how they could be manipulated to prevent complications like excessive granulation tissue growth.

Cite This Article

APA
Miragliotta V, Ipiña Z, Lefebvre-Lavoie J, Lussier JG, Theoret CL. (2008). Equine CTNNB1 and PECAM1 nucleotide structure and expression analyses in an experimental model of normal and pathological wound repair. BMC Physiol, 8, 1. https://doi.org/10.1186/1472-6793-8-1

Publication

BMC physiology
ISSN: 1472-6793
NlmUniqueID: 101088687
Country: England
Language: English
Volume: 8
Pages: 1

Researcher Affiliations

Miragliotta, Vincenzo
  • Département de biomédecine vétérinaire, Faculté de médecine vétérinaire, Université de Montréal, C,P, 5000, St-Hyacinthe, Québec, J2S 7C6, Canada. vincenzo.miragliotta@vet.unipi.it
Ipiña, Zoë
    Lefebvre-Lavoie, Josiane
      Lussier, Jacques G
        Theoret, Christine L

          MeSH Terms

          • Animals
          • Base Sequence
          • Cicatrix / genetics
          • Gene Expression / genetics
          • Gene Expression Regulation / genetics
          • Horses / genetics
          • Molecular Sequence Data
          • Platelet Endothelial Cell Adhesion Molecule-1 / genetics
          • Reference Values
          • Wound Healing / genetics
          • beta Catenin / genetics

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          Citations

          This article has been cited 3 times.
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