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Equine epidermis: a source of epithelial-like stem/progenitor cells with in vitro and in vivo regenerative capacities.
Abstract: Besides the presence of somatic stem cells in hair follicles and dermis, the epidermis also contains a subpopulation of stem cells, reflecting its high regenerative capacity. However, only limited information concerning epidermis-derived epithelial-like stem/progenitor cells (EpSCs) is available to date. Nonetheless, this stem cell type could prove itself useful in skin reconstitution after injury. After harvesting from equine epidermis, the purified cells were characterized as EpSCs by means of positive expression for CD29, CD44, CD49f, CD90, Casein Kinase 2β, p63, and Ki67, low expression for cytokeratin (CK)14 and negative expression for CD105, CK18, Wide CK, and Pan CK. Furthermore, their self-renewal capacity was assessed in adhesion as well as in suspension. Moreover, the isolated cells were differentiated toward keratinocytes and adipocytes. To assess the regenerative capacities of EpSCs, six full-thickness skin wounds were made: three were treated with EpSCs and platelet-rich-plasma (EpSC/PRP-treated), while the remaining three were administered carrier fluid alone (PRP-treated). The dermis of EpSC/PRP-treated wounds was significantly thinner and exhibited more restricted granulation tissue than did the PRP-treated wounds. The EpSC/PRP-treated wounds also exhibited increases in EpSCs, vascularization, elastin content, and follicle-like structures. In addition, combining EpSCs with a PRP treatment enhanced tissue repair after clinical application.
Publication Date: 2014-02-07 PubMed ID: 24368059DOI: 10.1089/scd.2013.0203Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study describes how researchers isolated and characterized skin stem cells from horse skin, demonstrating that these cells can be used to enhance the repair of full-thickness skin injuries in horses when paired with platelet-rich plasma treatments.
Overview of the Research
- This research explored the existence of a specific type of stem cells, epithelial-like stem/progenitor cells (EpSCs), in the skin (epidermis) of horses. These stem cells have a high capacity for regeneration and could therefore be highly beneficial for skin repair following injury.
- The researchers harvested these EpSCs from the epidermis of horses, subsequently purified them, and characterized the EpSCs based on their expression of certain protein markers.
Characterization of EpSCs
- The characterization process involved identifying the markers that the cells express. These markers included CD29, CD44, CD49f, CD90, Casein Kinase 2β, p63, and Ki67, all of which were positively expressed. The cells showed low expression for cytokeratin (CK)14 and were negative for CD105, CK18, Wide CK, and Pan CK.
- Assessing the self-renewal capacity of these cells, the researchers conducted further tests in adhesion and suspension conditions.
- Following this, the researchers induced the EpSCs to differentiate into two different cell types: keratinocytes, which are skin cells, and adipocytes, which are fat cells. This process confirmed the multipotency of the stem cells.
Application and Assessment of Regenerative Capacities
- The regenerative capacity of the EpSCs was tested by inducing full-thickness skin wounds on a horse and treating some wounds with a combination of EpSCs and platelet-rich-plasma (PRP), while treating others with just the carrier fluid.
- The wounds treated with the combination of EpSCs and PRP showed boosted improvement. Specifically, they exhibited a thinner dermis, restricted granulation tissue, increased numbers of EpSCs, increased vascularization, greater elastin content, and more follicle-like structures than the wounds treated with just the carrier fluid.
- The finding suggests that treating wounds with a combination of EpSCs and PRP can enhance tissue repair after clinical application.
Cite This Article
APA
Broeckx SY, Maes S, Martinello T, Aerts D, Chiers K, Mariu00ebn T, Patruno M, Franco-Obregu00f3n A, Spaas JH.
(2014).
Equine epidermis: a source of epithelial-like stem/progenitor cells with in vitro and in vivo regenerative capacities.
Stem Cells Dev, 23(10), 1134-1148.
https://doi.org/10.1089/scd.2013.0203 Publication
Researcher Affiliations
- 1 Global Stem cell Technology, Meldert-Lummen , Belgium .
MeSH Terms
- Animals
- Antigens, Differentiation / metabolism
- Cell Differentiation
- Epidermal Cells
- Epidermis / metabolism
- Epidermis / transplantation
- Horses
- Keratinocytes / cytology
- Keratinocytes / metabolism
- Regeneration
- Stem Cell Transplantation
- Stem Cells / cytology
- Stem Cells / metabolism
- Wounds and Injuries / metabolism
- Wounds and Injuries / pathology
- Wounds and Injuries / therapy
Citations
This article has been cited 9 times.- Depuydt E, Broeckx SY, Chiers K, Patruno M, Da Dalt L, Duchateau L, Saunders J, Pille F, Martens A, Van Hecke L, Spaas JH. Cellular and Humoral Immunogenicity Investigation of Single and Repeated Allogeneic Tenogenic Primed Mesenchymal Stem Cell Treatments in Horses Suffering From Tendon Injuries.. Front Vet Sci 2021;8:789293.
- Choi S, Yoon M, Choi KY. Approaches for Regenerative Healing of Cutaneous Wound with an Emphasis on Strategies Activating the Wnt/u03b2-Catenin Pathway.. Adv Wound Care (New Rochelle) 2022 Feb;11(2):70-86.
- Yang Q, Pinto VMR, Duan W, Paxton EE, Dessauer JH, Ryan W, Lopez MJ. In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates.. Front Bioeng Biotechnol 2019;7:155.
- Yang Q, Lopez MJ. Ultrastructural morphology is distinct among primary progenitor cell isolates from normal, inflamed, and cryopreserved equine hoof tissue and CD105(+)K14(+) progenitor cells.. In Vitro Cell Dev Biol Anim 2019 Sep;55(8):641-655.
- He B, Chen J, Liu L, Wang H, Wang S, Li P, Zhou J. Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats.. Biosci Rep 2019 Aug 30;39(8).
- Xian W, Duleba M, Yamamoto Y, Vincent M, McKeon F. Biobanking Organoids or Ground-State Stem Cells?. J Clin Med 2018 Dec 16;7(12).
- Textor JA, Clark KC, Walker NJ, Aristizobal FA, Kol A, LeJeune SS, Bledsoe A, Davidyan A, Gray SN, Bohannon-Worsley LK, Woolard KD, Borjesson DL. Allogeneic Stem Cells Alter Gene Expression and Improve Healing of Distal Limb Wounds in Horses.. Stem Cells Transl Med 2018 Jan;7(1):98-108.
- Linardi RL, Megee SO, Mainardi SR, Senoo M, Galantino-Homer HL. Expression and localization of epithelial stem cell and differentiation markers in equine skin, eye and hoof.. Vet Dermatol 2015 Aug;26(4):213-e47.
- Song M, Lim J, Yu HY, Park J, Chun JY, Jeong J, Heo J, Kang H, Kim Y, Cho YM, Kim SW, Oh W, Choi SJ, Jang SW, Park S, Shin DM, Choo MS. Mesenchymal Stem Cell Therapy Alleviates Interstitial Cystitis by Activating Wnt Signaling Pathway.. Stem Cells Dev 2015 Jul 15;24(14):1648-57.
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