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Home / Equine Research Bank / Stem Cell Rev Rep / Equine Hoof Stem Progenitor Cells (HPC) CD29 +&#x2009...
Stem cell reviews and reports2021; 17(4); 1478-1485; doi: 10.1007/s12015-021-10187-x

Equine Hoof Stem Progenitor Cells (HPC) CD29 + /Nestin + /K15 + - a Novel Dermal/epidermal Stem Cell Population With a Potential Critical Role for Laminitis Treatment.

Abstract: Laminitis is a life threating, extremely painful and frequently recurrent disease of horses which affects hoof structure. It results from the disruption of blood flow to the laminae, contributing to laminitis and in severe separation of bone from the hoof capsule. Still, the pathophysiology of the disease remains unclear, mainly due to its complexity. In the light of the presented data, in the extremally difficult process of tissue structure restoration after disruption, a novel type of progenitor cells may be involved. Herein, we isolated and performed the initial characterization of stem progenitor cells isolated from the coronary corium of the equine feet (HPC). Phenotype of the cells was investigated with flow cytometry and RT-qPCR revealing the presence of nestin, CD29, and expression of progenitor cell markers including SOX2, OCT4, NANOG and K14. Morphology of HPC was investigated with light, confocal and SEM microscopes. Cultured cells were characterised by spindle shaped morphology, eccentric nuclei, elongated mitochondria, and high proliferation rate. Plasticity and multilineage differentiation potential was confirmed by specific staining and gene expression analysis. We conclude that HPC exhibit in vitro expansion and plasticity similar to mesenchymal stem cells, which can be isolated from the equine foot, and may be directly involved in the pathogenesis and recovery of laminitis. Obtained results are of importance to the field of laminitis treatment as determining the repairing cell populations could contribute to the discovery of novel therapeutic targets and agents including and cell-based therapies for affected animals.
© 2021. The Author(s).
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Publication Date: 2021-05-26 PubMed ID: 34037924PubMed Central: PMC8149919DOI: 10.1007/s12015-021-10187-xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study has identified a new kind of progenitor cells in horses which can potentially help in treating an extremely painful disease known as laminitis that affects the hoof structure of horses.

Overview of the Research

This study delves into the exploration of the pathophysiology of laminitis, a severe and painful disease in horses affecting the structure of their hooves. The disease is caused by a disruption of blood flow which often leads to the separation of bone from the hoof in severe cases. The researchers mention the complexity of the disease, which is a significant factor for its unclear nature. Aimed at unlocking new therapeutic alternatives, this study explores a new kind of progenitor cells that could play a crucial role in tissue structure restoration, thus contributing towards the treatment of laminitis.

Identification and Characterization of HPC

  • The researchers initially isolated these unique progenitor cells from the coronary corium of the equine feet, referring to these as hoof progenitor cells (HPC)
  • Following isolation, the HPC’s phenotype was analyzed using flow cytometry and RT-qPCR which revealed the presence of nestin, CD29 and the expression of progenitor cell markers such as SOX2, OCT4, NANOG and K14.
  • In addition, morphology of HPC was studied using different kinds of microscopes. The characteristics of HPC include spindle-shaped morphology, eccentric nuclei, elongated mitochondria, and high proliferation rate.

Potential of HPC in Laminitis Treatment

  • The researchers found out that these cells showcase a plasticity pattern and multilineage differentiation potential, which mirrors the mesenchymal stem cells.
  • This discovery suggests that HPC obtained from the equine foot could play a significant role in both pathogenesis and recovery of laminitis.
  • The results obtained from the study highlight the importance of identifying the repairing cell populations that could pave the way for discovering new therapeutic targets for laminitis treatment.
  • Finding such cell-based therapies for laminitis not only present a novel direction for laminitis treatment, but also enrich the understanding of this complex disease.

Cite This Article

APA
Marycz K, Pielok A, Kornicka-Garbowska K. (2021). Equine Hoof Stem Progenitor Cells (HPC) CD29 + /Nestin + /K15 + - a Novel Dermal/epidermal Stem Cell Population With a Potential Critical Role for Laminitis Treatment. Stem Cell Rev Rep, 17(4), 1478-1485. https://doi.org/10.1007/s12015-021-10187-x

Publication

Stem cell reviews and reports
ISSN: 2629-3277
NlmUniqueID: 101752767
Country: United States
Language: English
Volume: 17
Issue: 4
Pages: 1478-1485

Researcher Affiliations

Marycz, Krzysztof
  • International Institute of Translational Medicine (MIMT), ul. Jesionowa 11, 55-114, Malin Wisznia Mała, Poland. krzysztofmarycz@interia.pl.
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, ul. CK Norwida 27, 50-375, Wrocław, Poland. krzysztofmarycz@interia.pl.
Pielok, Ariadna
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, ul. CK Norwida 27, 50-375, Wrocław, Poland.
Kornicka-Garbowska, Katarzyna
  • International Institute of Translational Medicine (MIMT), ul. Jesionowa 11, 55-114, Malin Wisznia Mała, Poland.
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, ul. CK Norwida 27, 50-375, Wrocław, Poland.

MeSH Terms

  • Animals
  • Foot Diseases / therapy
  • Foot Diseases / veterinary
  • Hoof and Claw / cytology
  • Horse Diseases / genetics
  • Horse Diseases / therapy
  • Horses
  • Integrin beta1
  • Keratins
  • Nestin
  • Stem Cells / cytology

Conflict of Interest Statement

Authors declare that there is no conflict of interest.

References

This article includes 29 references
  1. Carmalt JL. Equine Laminitis. The. Canadian Veterinary Journal 2018;59(4):407.
  2. Robertson TP, Bailey SR, Peroni JF. Equine laminitis: a journey to the dark side of venous.. Vet Immunol Immunopathol 2009 Jun 15;129(3-4):164-6.
    doi: 10.1016/j.vetimm.2008.11.015pubmed: 19110318google scholar: lookup
  3. Stokes AM, Keowen ML, McGeachy M, Carlisle K, Garza F. Potential Role of the Toll-Like Receptor Signaling Pathway in Equine Laminitis. Journal of Equine Veterinary Science 2010;30(2):113–114.
    doi: 10.1016/j.jevs.2010.01.038google scholar: lookup
  4. . Laminitis and the Equine Metabolic Syndrome. .
  5. Johnson PJ, Wiedmeyer CE, LaCarrubba A, Ganjam VK, Messer NT 4th. Laminitis and the equine metabolic syndrome.. Vet Clin North Am Equine Pract 2010 Aug;26(2):239-55.
    doi: 10.1016/j.cveq.2010.04.004pubmed: 20699172google scholar: lookup
  6. Galkowski D, Ratajczak MZ, Kocki J, Darzynkiewicz Z. Of Cytometry, Stem Cells and Fountain of Youth.. Stem Cell Rev Rep 2017 Aug;13(4):465-481.
    doi: 10.1007/s12015-017-9733-5pubmed: 28364326google scholar: lookup
  7. Marycz K, Kornicka K, Basinska K, Czyrek A. Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging.. Oxid Med Cell Longev 2016;2016:4710326.
    doi: 10.1155/2016/4710326pmc: PMC4670679pubmed: 26682006google scholar: lookup
  8. Kornicka K, Geburek F, Röcken M, Marycz K. Stem Cells in Equine Veterinary Practice-Current Trends, Risks, and Perspectives.. J Clin Med 2019 May 14;8(5).
    pmc: PMC6572129pubmed: 31091732doi: 10.3390/jcm8050675google scholar: lookup
  9. Kornicka K, Szłapka-Kosarzewska J, Śmieszek A, Marycz K. 5-Azacytydine and resveratrol reverse senescence and ageing of adipose stem cells via modulation of mitochondrial dynamics and autophagy.. J Cell Mol Med 2019 Jan;23(1):237-259.
    doi: 10.1111/jcmm.13914pmc: PMC6307768pubmed: 30370650google scholar: lookup
  10. Akyurekli C, Le Y, Richardson RB, Fergusson D, Tay J, Allan DS. A systematic review of preclinical studies on the therapeutic potential of mesenchymal stromal cell-derived microvesicles.. Stem Cell Rev Rep 2015 Feb;11(1):150-60.
    doi: 10.1007/s12015-014-9545-9pubmed: 25091427google scholar: lookup
  11. Collino F, Pomatto M, Bruno S, Lindoso RS, Tapparo M, Sicheng W, Quesenberry P, Camussi G. Exosome and Microvesicle-Enriched Fractions Isolated from Mesenchymal Stem Cells by Gradient Separation Showed Different Molecular Signatures and Functions on Renal Tubular Epithelial Cells.. Stem Cell Rev Rep 2017 Apr;13(2):226-243.
    doi: 10.1007/s12015-016-9713-1pmc: PMC5380712pubmed: 28070858google scholar: lookup
  12. Shi J, Lv Z, Nie M, Lu W, Liu C, Tian Y, Li L, Zhang G, Ren R, Zhang Z, Kang H. Human nail stem cells are retained but hypofunctional during aging.. J Mol Histol 2018 Jun;49(3):303-316.
    doi: 10.1007/s10735-018-9769-0pmc: PMC5942356pubmed: 29633149google scholar: lookup
  13. Al Naem M, Bourebaba L, Kucharczyk K, Röcken M, Marycz K. Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders.. Stem Cell Rev Rep 2020 Apr;16(2):301-322.
    doi: 10.1007/s12015-019-09932-0pubmed: 31797146google scholar: lookup
  14. Yang Q, Pinto VMR, Duan W, Paxton EE, Dessauer JH, Ryan W, Lopez MJ. In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates.. Front Bioeng Biotechnol 2019;7:155.
    pmc: PMC6637248pubmed: 31355191doi: 10.3389/fbioe.2019.00155google scholar: lookup
  15. Seweryn A, Pielok A, Lawniczak-Jablonska K, Pietruszka R, Marcinkowska K, Sikora M, Witkowski BS, Godlewski M, Marycz K, Smieszek A. Zirconium Oxide Thin Films Obtained by Atomic Layer Deposition Technology Abolish the Anti-Osteogenic Effect Resulting from miR-21 Inhibition in the Pre-Osteoblastic MC3T3 Cell Line.. Int J Nanomedicine 2020;15:1595-1610.
    doi: 10.2147/IJN.S237898pmc: PMC7069564pubmed: 32210554google scholar: lookup
  16. Marędziak M, Marycz K, Tomaszewski KA, Kornicka K, Henry BM. The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells.. Stem Cells Int 2016;2016:2152435.
    pmc: PMC4749808pubmed: 26941800doi: 10.1155/2016/2152435google scholar: lookup
  17. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.. Anal Biochem 1987 Apr;162(1):156-9.
    doi: 10.1006/abio.1987.9999pubmed: 2440339google scholar: lookup
  18. Suszynska M, Poniewierska-Baran A, Gunjal P, Ratajczak J, Marycz K, Kakar SS, Kucia M, Ratajczak MZ. Expression of the erythropoietin receptor by germline-derived cells - further support for a potential developmental link between the germline and hematopoiesis.. J Ovarian Res 2014;7:66.
    doi: 10.1186/1757-2215-7-66pmc: PMC4074848pubmed: 24982693google scholar: lookup
  19. Marycz K, Szłapka-Kosarzewska J, Geburek F, Kornicka-Garbowska K. Systemic Administration of Rejuvenated Adipose-Derived Mesenchymal Stem Cells Improves Liver Metabolism in Equine Metabolic Syndrome (EMS)- New Approach in Veterinary Regenerative Medicine.. Stem Cell Rev Rep 2019 Dec;15(6):842-850.
    doi: 10.1007/s12015-019-09913-3pmc: PMC6925066pubmed: 31620992google scholar: lookup
  20. Kornicka-Garbowska K, Pędziwiatr R, Woźniak P, Kucharczyk K, Marycz K. Microvesicles isolated from 5-azacytidine-and-resveratrol-treated mesenchymal stem cells for the treatment of suspensory ligament injury in horse-a case report.. Stem Cell Res Ther 2019 Dec 18;10(1):394.
    pmc: PMC6921487pubmed: 31852535doi: 10.1186/s13287-019-1469-5google scholar: lookup
  21. Fiore EJ, Mazzolini G, Aquino JB. Mesenchymal Stem/Stromal Cells in Liver Fibrosis: Recent Findings, Old/New Caveats and Future Perspectives.. Stem Cell Rev Rep 2015 Aug;11(4):586-97.
    doi: 10.1007/s12015-015-9585-9pubmed: 25820543google scholar: lookup
  22. Gazdic M, Volarevic V, Arsenijevic N, Stojkovic M. Mesenchymal stem cells: a friend or foe in immune-mediated diseases.. Stem Cell Rev Rep 2015 Apr;11(2):280-7.
    doi: 10.1007/s12015-014-9583-3pubmed: 25592610google scholar: lookup
  23. Meyer KC, Klatte JE, Dinh HV, Harries MJ, Reithmayer K, Meyer W, Sinclair R, Paus R. Evidence that the bulge region is a site of relative immune privilege in human hair follicles.. Br J Dermatol 2008 Nov;159(5):1077-85.
    doi: 10.1111/j.1365-2133.2008.08818.xpubmed: 18795933google scholar: lookup
  24. Rieger J, Kaessmeyer S, Al Masri S, Hünigen H, Plendl J. Endothelial cells and angiogenesis in the horse in health and disease-A review.. Anat Histol Embryol 2020 Sep;49(5):656-678.
    doi: 10.1111/ahe.12588pubmed: 32639627google scholar: lookup
  25. Gomei Y, Nakamura Y, Yoshihara H, Hosokawa K, Iwasaki H, Suda T, Arai F. Functional differences between two Tie2 ligands, angiopoietin-1 and -2, in regulation of adult bone marrow hematopoietic stem cells.. Exp Hematol 2010 Feb;38(2):82-9.
    doi: 10.1016/j.exphem.2009.11.007pubmed: 19945502google scholar: lookup
  26. Liu Y, Lyle S, Yang Z, Cotsarelis G. Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge.. J Invest Dermatol 2003 Nov;121(5):963-8.
    doi: 10.1046/j.1523-1747.2003.12600.xpubmed: 14708593google scholar: lookup
  27. Lyle S, Christofidou-Solomidou M, Liu Y, Elder DE, Albelda S, Cotsarelis G. The C8/144B monoclonal antibody recognizes cytokeratin 15 and defines the location of human hair follicle stem cells.. J Cell Sci 1998 Nov;111 ( Pt 21):3179-88.
    doi: 10.1242/jcs.111.21.3179pubmed: 9763512google scholar: lookup
  28. Waseem A, Dogan B, Tidman N, Alam Y, Purkis P, Jackson S, Lalli A, Machesney M, Leigh IM. Keratin 15 expression in stratified epithelia: downregulation in activated keratinocytes.. J Invest Dermatol 1999 Mar;112(3):362-9.
    doi: 10.1046/j.1523-1747.1999.00535.xpubmed: 10084315google scholar: lookup
  29. Yang Q, Pinto VMR, Duan W, Paxton EE, Dessauer JH, Ryan W, Lopez MJ. In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates.. Front Bioeng Biotechnol 2019;7:155.
    pmc: PMC6637248pubmed: 31355191doi: 10.3389/fbioe.2019.00155google scholar: lookup

Citations

This article has been cited 2 times.
  1. Pielok A, Kępska M, Steczkiewicz Z, Grobosz S, Bourebaba L, Marycz K. Equine Hoof Progenitor Cells Display Increased Mitochondrial Metabolism and Adaptive Potential to a Highly Pro-Inflammatory Microenvironment. Int J Mol Sci 2023 Jul 14;24(14).
    doi: 10.3390/ijms241411446pubmed: 37511204google scholar: lookup
  2. Smieszek A, Marcinkowska K, Pielok A, Sikora M, Valihrach L, Carnevale E, Marycz K. Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism. Cells 2022 Apr 24;11(9).
    doi: 10.3390/cells11091437pubmed: 35563743google scholar: lookup
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