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Stem cell reviews and reports2021; 17(4); 1478-1485; doi: 10.1007/s12015-021-10187-x

Equine Hoof Stem Progenitor Cells (HPC) CD29 + /Nestin + /K15 + - a Novel Dermal/epidermal Stem Cell Population With a Potential Critical Role for Laminitis Treatment.

Abstract: Laminitis is a life threating, extremely painful and frequently recurrent disease of horses which affects hoof structure. It results from the disruption of blood flow to the laminae, contributing to laminitis and in severe separation of bone from the hoof capsule. Still, the pathophysiology of the disease remains unclear, mainly due to its complexity. In the light of the presented data, in the extremally difficult process of tissue structure restoration after disruption, a novel type of progenitor cells may be involved. Herein, we isolated and performed the initial characterization of stem progenitor cells isolated from the coronary corium of the equine feet (HPC). Phenotype of the cells was investigated with flow cytometry and RT-qPCR revealing the presence of nestin, CD29, and expression of progenitor cell markers including SOX2, OCT4, NANOG and K14. Morphology of HPC was investigated with light, confocal and SEM microscopes. Cultured cells were characterised by spindle shaped morphology, eccentric nuclei, elongated mitochondria, and high proliferation rate. Plasticity and multilineage differentiation potential was confirmed by specific staining and gene expression analysis. We conclude that HPC exhibit in vitro expansion and plasticity similar to mesenchymal stem cells, which can be isolated from the equine foot, and may be directly involved in the pathogenesis and recovery of laminitis. Obtained results are of importance to the field of laminitis treatment as determining the repairing cell populations could contribute to the discovery of novel therapeutic targets and agents including and cell-based therapies for affected animals.
© 2021. The Author(s).
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Publication Date: 2021-05-26 PubMed ID: 34037924PubMed Central: PMC8149919DOI: 10.1007/s12015-021-10187-xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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The study has identified a new kind of progenitor cells in horses which can potentially help in treating an extremely painful disease known as laminitis that affects the hoof structure of horses.

Overview of the Research

This study delves into the exploration of the pathophysiology of laminitis, a severe and painful disease in horses affecting the structure of their hooves. The disease is caused by a disruption of blood flow which often leads to the separation of bone from the hoof in severe cases. The researchers mention the complexity of the disease, which is a significant factor for its unclear nature. Aimed at unlocking new therapeutic alternatives, this study explores a new kind of progenitor cells that could play a crucial role in tissue structure restoration, thus contributing towards the treatment of laminitis.

Identification and Characterization of HPC

  • The researchers initially isolated these unique progenitor cells from the coronary corium of the equine feet, referring to these as hoof progenitor cells (HPC)
  • Following isolation, the HPC’s phenotype was analyzed using flow cytometry and RT-qPCR which revealed the presence of nestin, CD29 and the expression of progenitor cell markers such as SOX2, OCT4, NANOG and K14.
  • In addition, morphology of HPC was studied using different kinds of microscopes. The characteristics of HPC include spindle-shaped morphology, eccentric nuclei, elongated mitochondria, and high proliferation rate.

Potential of HPC in Laminitis Treatment

  • The researchers found out that these cells showcase a plasticity pattern and multilineage differentiation potential, which mirrors the mesenchymal stem cells.
  • This discovery suggests that HPC obtained from the equine foot could play a significant role in both pathogenesis and recovery of laminitis.
  • The results obtained from the study highlight the importance of identifying the repairing cell populations that could pave the way for discovering new therapeutic targets for laminitis treatment.
  • Finding such cell-based therapies for laminitis not only present a novel direction for laminitis treatment, but also enrich the understanding of this complex disease.

Cite This Article

APA
Marycz K, Pielok A, Kornicka-Garbowska K. (2021). Equine Hoof Stem Progenitor Cells (HPC) CD29 + /Nestin + /K15 + - a Novel Dermal/epidermal Stem Cell Population With a Potential Critical Role for Laminitis Treatment. Stem Cell Rev Rep, 17(4), 1478-1485. https://doi.org/10.1007/s12015-021-10187-x

Publication

Stem cell reviews and reports
ISSN: 2629-3277
NlmUniqueID: 101752767
Country: United States
Language: English
Volume: 17
Issue: 4
Pages: 1478-1485

Researcher Affiliations

Marycz, Krzysztof
  • International Institute of Translational Medicine (MIMT), ul. Jesionowa 11, 55-114, Malin Wisznia Mała, Poland. krzysztofmarycz@interia.pl.
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, ul. CK Norwida 27, 50-375, Wrocław, Poland. krzysztofmarycz@interia.pl.
Pielok, Ariadna
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, ul. CK Norwida 27, 50-375, Wrocław, Poland.
Kornicka-Garbowska, Katarzyna
  • International Institute of Translational Medicine (MIMT), ul. Jesionowa 11, 55-114, Malin Wisznia Mała, Poland.
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, ul. CK Norwida 27, 50-375, Wrocław, Poland.

MeSH Terms

  • Animals
  • Foot Diseases / therapy
  • Foot Diseases / veterinary
  • Hoof and Claw / cytology
  • Horse Diseases / genetics
  • Horse Diseases / therapy
  • Horses
  • Integrin beta1
  • Keratins
  • Nestin
  • Stem Cells / cytology

Conflict of Interest Statement

Authors declare that there is no conflict of interest.

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Citations

This article has been cited 2 times.
  1. Pielok A, Kępska M, Steczkiewicz Z, Grobosz S, Bourebaba L, Marycz K. Equine Hoof Progenitor Cells Display Increased Mitochondrial Metabolism and Adaptive Potential to a Highly Pro-Inflammatory Microenvironment. Int J Mol Sci 2023 Jul 14;24(14).
    doi: 10.3390/ijms241411446pubmed: 37511204google scholar: lookup
  2. Smieszek A, Marcinkowska K, Pielok A, Sikora M, Valihrach L, Carnevale E, Marycz K. Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism. Cells 2022 Apr 24;11(9).
    doi: 10.3390/cells11091437pubmed: 35563743google scholar: lookup
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