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Home / Equine Research Bank / J Virol / Equine infectious anemia virus: evidence favoring classifica...
Journal of virology1976; 19(3); 1073-1079; doi: 10.1128/JVI.19.3.1073-1079.1976

Equine infectious anemia virus: evidence favoring classification as a retravirus.

Abstract: Equine infectious anemia virus (EIAV) has a density of 1.154 g/cm3 in sucrose a high-molecular-weight RNA similar in size to Rauscher murine leukemia virus, and an internal virion reverse transcriptase that utilizes the synthetic RNA template poly(rA) but not the synthetic DNA template poly(dA), both with (dT)12 as primer. Although capable of utilizing manganese at low concentrations (approximately 0.1 mM), EIAV reverse transcriptase showed highest activity in the presence of 9 mM magnesium. The major protein of EIAV has a slightly lower molecular weight than the comparable protein of type C viruses and co-electrophoresed with 125I-labeled p25 of Mason-Pfizer monkey virus. A reference horse serum with antibodies to the major EIAV protein reacted only with EIAV and not with other type C or non-type C retraviruses. Reciprocally, a broadly reactive serum to type C virus p30s and specific sera to a variety of non-type C retraviruses did not react with EIAV. We recommend the inclusion of EIAV in the family Retraviridae.
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Publication Date: 1976-09-01 PubMed ID: 61283PubMed Central: PMC354947DOI: 10.1128/JVI.19.3.1073-1079.1976Google Scholar: Lookup
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  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper presents evidence supporting the classification of Equine infectious anemia virus (EIAV) as a retravirus, based on its molecular characteristics and reaction with horse serum with antibodies to EIAV protein only.

Research Methodology

  • The research utilized the tools of molecular biology to study the Equine infectious anemia virus (EIAV).
  • Specifically, the researchers examined the density of EIAV, its RNA size, and the activity of its internal virion reverse transcriptase which uses a synthetic RNA template.
  • The reverse transcriptase was also tested with different concentrations of manganese and magnesium to examine any variations in activity.
  • In addition to this, the researchers compared the molecular weight of EIAV’s major protein with those of other viruses and tracked its interaction with a specific horse serum.

Key Findings

  • The researchers established that EIAV has a density of 1.154 g/cm3 in sucrose and a high-molecular-weight RNA similar in size to Rauscher murine leukemia virus.
  • The EIAV reverse transcriptase showed highest activity when 9 mM of magnesium was present, though it could utilize manganese at low concentrations –approximately 0.1 mM.
  • The major protein in EIAV had a slightly lower molecular weight than other type C viruses, and it showed similar properties to a protein in the Mason-Pfizer monkey virus during co-electrophoresis.
  • The horse serum with antibodies reacted only with EIAV and not with other type C or non-type C retraviruses. Similarly, a serum reactant to type C virus and other non-type C retraviruses did not react with EIAV.

Conclusion

  • The research provided substantial evidence to recommend the inclusion of EIAV in the family of Retraviridae.
  • This reclassification is supported by the unique interactions of EIAV with specific horse serum, its particular RNA size and density, and the specific activity of its reverse transcriptase in the presence of magnesium.

Cite This Article

APA
Charman HP, Bladen S, Gilden RV, Coggins L. (1976). Equine infectious anemia virus: evidence favoring classification as a retravirus. J Virol, 19(3), 1073-1079. https://doi.org/10.1128/JVI.19.3.1073-1079.1976

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 19
Issue: 3
Pages: 1073-1079

Researcher Affiliations

Charman, H P
    Bladen, S
      Gilden, R V
        Coggins, L

          MeSH Terms

          • Antigens, Viral / analysis
          • Infectious Anemia Virus, Equine / analysis
          • Infectious Anemia Virus, Equine / classification
          • Infectious Anemia Virus, Equine / immunology
          • Oncogenic Viruses / analysis
          • Oncogenic Viruses / classification
          • Oncogenic Viruses / immunology
          • Peptides / analysis
          • RNA, Viral / analysis
          • RNA-Directed DNA Polymerase / analysis
          • Viral Proteins / analysis

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