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Methods in molecular biology (Clifton, N.J.)2014; 1161; 371-377; doi: 10.1007/978-1-4939-0758-8_32

Equine influenza diagnosis: sample collection and transport.

Abstract: In horses, presumptive diagnosis of equine influenza is commonly made on the basis of clinical signs. This alone is insufficient for confirmation of equine influenza, because other equine infectious respiratory diseases can in some degree have similar clinical presentations. Surveillance and control of equine influenza also necessitate detection of subclinical cases. Effective diagnosis of equine influenza virus infection is critically dependent on obtaining adequate specimens of virus-containing respiratory secretions for testing. These specimens are also valuable as sources for isolation of virus strains for antigenic characterization and potential inclusion in vaccines. Both nasal swabs and nasopharyngeal swabs are employed in horses. These differ little in their invasiveness, but nasopharyngeal swabs typically yield more virus than nasal swabs and are superior diagnostic specimens. Methods for obtaining nasopharyngeal swab specimens are described.
Publication Date: 2014-06-06 PubMed ID: 24899446DOI: 10.1007/978-1-4939-0758-8_32Google Scholar: Lookup
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  • Journal Article

Summary

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The research article primarily deals with the specific methodology of diagnosis for equine influenza virus in horses, arguing that clinical signs alone are insufficient. It emphasizes the importance of obtaining proper specimens from horse’s respiratory secretions in order to effectively test for, monitor, and potentially prevent the virus. The article also indicates that nasopharyngeal swabs are more preferable than nasal swabs for this purpose.

Diagnosis of Equine Influenza

The article highlights the importance of a thorough diagnosis process to confirm an infection of equine influenza. It underlines that:

  • The current presumptive diagnosis is often based merely on clinical signs, which is insufficient to accurately confirm cases of equine influenza.
  • This is because many other infectious respiratory diseases affecting horses can present similar clinical signs, creating the risk of misdiagnosis.

The Need for Better Surveillance and Control

Improved and more accurate diagnosis methods are essential to better control and monitor equine influenza because:

  • Detection of subclinical cases, which may not present obvious signs or symptoms, is necessary for effective control of the disease.
  • Adequate specimens that contain the virus are critical for accurate testing and diagnosis.

Role of Respiratory Secretions

The collection of specimens from the horse’s respiratory secretions has multiple benefits:

  • Respiratory secretions serve as an effective source for virus isolation.
  • The isolated strains can be antigenically characterized.
  • These characterizations can contribute to the development of vaccines, helping to prevent further spread of the disease.

Comparison between Nasal Swabs and Nasopharyngeal Swabs

The article suggests a preference for using nasopharyngeal swabs over nasal swabs due to:

  • Similar invasiveness between the two methods, meaning neither is significantly more uncomfortable for the horse.
  • The superior efficacy of nasopharyngeal swabs in yielding more abundant virus samples, making them better specimens for diagnosis.
  • The methodology for obtaining nasopharyngeal swab specimens is explained within the article, shedding light on how practitioners can obtain more effective samples.

Cite This Article

APA
Chambers TM, Reedy SE. (2014). Equine influenza diagnosis: sample collection and transport. Methods Mol Biol, 1161, 371-377. https://doi.org/10.1007/978-1-4939-0758-8_32

Publication

ISSN: 1940-6029
NlmUniqueID: 9214969
Country: United States
Language: English
Volume: 1161
Pages: 371-377

Researcher Affiliations

Chambers, Thomas M
  • Department of Veterinary Science, OIE Reference Laboratory for Equine Influenza, Maxwell H. Gluck Equine Research Center, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546-0099, USA, tmcham1@uky.edu.
Reedy, Stephanie E

    MeSH Terms

    • Animals
    • Horse Diseases / diagnosis
    • Horse Diseases / virology
    • Horses / virology
    • Nasopharynx / virology
    • Orthomyxoviridae Infections / diagnosis
    • Orthomyxoviridae Infections / veterinary
    • Specimen Handling / methods

    Citations

    This article has been cited 1 times.
    1. Singh RK, Dhama K, Karthik K, Khandia R, Munjal A, Khurana SK, Chakraborty S, Malik YS, Virmani N, Singh R, Tripathi BN, Munir M, van der Kolk JH. A Comprehensive Review on Equine Influenza Virus: Etiology, Epidemiology, Pathobiology, Advances in Developing Diagnostics, Vaccines, and Control Strategies. Front Microbiol 2018;9:1941.
      doi: 10.3389/fmicb.2018.01941pubmed: 30237788google scholar: lookup