Equine mesenchymal stem cells derived from endometrial or adipose tissue share significant biological properties, but have distinctive pattern of surface markers and migration.
Abstract: Adult stromal mesenchymal stem cells (MSCs) have been postulated as responsible for cell renewal in highly and continuously regenerative tissues such as the endometrium. MSCs have been identified in the endometrium of many species including humans, rodents, pets and some farm animals, but not in horses. The objective of this work was to isolate such cells from the endometrium of mares and to compare their main biological attributes with horse adipose-derived MSCs. Here we successfully isolated and characterized endometrial MSCs (eMSCs) from mares. Said cells showed fibroblast-like morphology, grew on plastic, had doubling population times of 46.4 ± 3.38 h, underwent tri-lineage (osteo, chondro and adipogenic) differentiation after appropriate inductions, migrated toward the attraction of fetal calf serum and displayed a pattern of surface markers commonly accepted for horse MSCs. All these are properties of MSCs. Some of these attributes were shared with equine adipose-derived MSCs, but the migration pattern of eMSC at 12 and 24 h after stimulation was reduced in comparison with adipose MSCs. Also, expression of CD44, CD90 and MHCI surface markers were dramatically down-regulated in eMSCs. In conclusion, equine-derived endometrial MSC share biological attributes with adipose MSC of this species, but displayed a different surface marker phenotype and an impaired migration ability. Conceivably, this phenotype is distinctive for MSC of this origin.
Copyright © 2017 Elsevier Inc. All rights reserved.
Publication Date: 2017-10-06 PubMed ID: 29049924DOI: 10.1016/j.theriogenology.2017.09.035Google Scholar: Lookup
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Summary
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This research identified and studied mesenchymal stem cells (MSCs) found in the endometrium of mares, compared them with those obtained from horse adipose tissue, uncovering notable biological similarities but distinct surface markers and migration patterns.
Objective and Method
- The main goal of this research was to isolate and identify mesenchymal stem cells (MSCs) from the endometrium of mares and compare their key biological attributes with MSCs obtained from horse adipose tissue. The study aimed to understand the features and behaviour of endometrial MSCs (eMSCs) versus adipose-derived MSCs.
Findings
- The researchers successfully isolated the eMSCs from mares. These cells showed fibroblast-like morphology, indicating that they closely resemble the structure of fibroblast cells which are essential for tissue repair in animals.
- The isolated eMSCs displayed characteristics typical of MSCs, such as the ability to grow on plastic, to differentiate into different types of cells (osteogenic, chondrogenic, and adipogenic), and to migrate toward the attraction of fetal calf serum.
- The study also found that the rate of doubling in population of eMSCs was approximately 46.4 ± 3.38 hours, providing insight into their multiplication speed.
- When the features of eMSCs were compared with those of MSCs derived from horse adipose tissue, several common attributes were observed. However, notable differences were also found – eMSCs displayed a reduced migration pattern and had dramatically down-regulated expression of surface markers CD44, CD90 and MHCI.
Conclusion
- In conclusion, the study demonstrates that eMSCs and adipose-derived MSCs in horses share several biological attributes, but differ significantly in terms of surface markers and migration ability. These differences suggest unique properties of MSCs arising from different origins – in this case, endometrium versus adipose tissue in horses.
Cite This Article
APA
Cabezas J, Rojas D, Navarrete F, Ortiz R, Rivera G, Saravia F, Rodriguez-Alvarez L, Castro FO.
(2017).
Equine mesenchymal stem cells derived from endometrial or adipose tissue share significant biological properties, but have distinctive pattern of surface markers and migration.
Theriogenology, 106, 93-102.
https://doi.org/10.1016/j.theriogenology.2017.09.035 Publication
Researcher Affiliations
- Universidad de Concepción, Campus Chillan, Faculty of Veterinary Sciences, Department of Animal Science, Laboratorio de Biotecnologia Animal, Chile. Electronic address: joecabezas@udec.cl.
- Universidad de Concepción, Campus Chillan, Faculty of Veterinary Sciences, Department of Animal Pathology, Chile. Electronic address: drojasm@udec.cl.
- Universidad de Concepción, Campus Chillan, Faculty of Veterinary Sciences, Department of Animal Science, Laboratorio de Biotecnologia Animal, Chile. Electronic address: fenavarr@udec.cl.
- Universidad de Concepción, Campus Chillan, Faculty of Veterinary Sciences, Department of Clinical Sciences, Hospital de Animales Mayores, Chile. Electronic address: reinaldortiz@udec.cl.
- Universidad de Concepción, Campus Chillan, Faculty of Veterinary Sciences, Department of Clinical Sciences, Hospital de Animales Mayores, Chile. Electronic address: gonrivera@udec.cl.
- Universidad de Concepción, Campus Chillan, Faculty of Veterinary Sciences, Department of Animal Science, Laboratorio de Biotecnologia Animal, Chile. Electronic address: fsaravia@udec.cl.
- Universidad de Concepción, Campus Chillan, Faculty of Veterinary Sciences, Department of Animal Science, Laboratorio de Biotecnologia Animal, Chile. Electronic address: llrodriguez@udec.cl.
- Universidad de Concepción, Campus Chillan, Faculty of Veterinary Sciences, Department of Animal Science, Laboratorio de Biotecnologia Animal, Chile. Electronic address: fidcastro@udec.cl.
MeSH Terms
- Adipose Tissue / cytology
- Animals
- Biomarkers
- Cell Movement
- Cells, Cultured
- Endometrium / cytology
- Female
- Gene Expression Regulation
- Horses / physiology
- Membrane Proteins / genetics
- Membrane Proteins / metabolism
- Mesenchymal Stem Cells / physiology
Citations
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