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Home / Equine Research Bank / J Biomed Mater Res B Appl Biomater / Equine model for soft-tissue regeneration.
Journal of biomedical materials research. Part B, Applied biomaterials2014; 103(6); 1217-1227; doi: 10.1002/jbm.b.33299

Equine model for soft-tissue regeneration.

Abstract: Soft-tissue regeneration methods currently yield suboptimal clinical outcomes due to loss of tissue volume and a lack of functional tissue regeneration. Grafted tissues and natural biomaterials often degrade or resorb too quickly, while most synthetic materials do not degrade. In previous research we demonstrated that soft-tissue regeneration can be supported using silk porous biomaterials for at least 18 months in vivo in a rodent model. In the present study, we scaled the system to a survival study using a large animal model and demonstrated the feasibility of these biomaterials for soft-tissue regeneration in adult horses. Both slow and rapidly degrading silk matrices were evaluated in subcutaneous pocket and intramuscular defect depots. We showed that we can effectively employ an equine model over 6 months to simultaneously evaluate many different implants, reducing the number of animals needed. Furthermore, we were able to tailor matrix degradation by varying the initial format of the implanted silk. Finally, we demonstrate ultrasound imaging of implants to be an effective means for tracking tissue regeneration and implant degradation.
© 2014 Wiley Periodicals, Inc.
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Publication Date: 2014-10-28 PubMed ID: 25350377PubMed Central: PMC4868549DOI: 10.1002/jbm.b.33299Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study discusses the use of silk porous biomaterials as potential solution for soft-tissue regeneration, detailing the success of these materials in an adult horse model. The researchers also highlight the efficiency of this model, demonstrating tailored matrix degradation and effective tracking of tissue regeneration through ultrasound imaging.

Research Background

  • The current techniques for soft-tissue regeneration often yield unsatisfactory clinical outcomes due to a rapid loss of tissue volume and insufficient functional tissue regeneration.
  • Grafted tissues and natural biomaterials often degrade or get absorbed too fast, while most synthetic materials do not degrade at all.
  • This research builds upon previous studies where the team demonstrated that using silk porous biomaterials can support soft-tissue regeneration for prolonged periods (at least 18 months) in a smaller, rodent model.

Purpose and Method of Study

  • The study’s aim was to validate and further investigate the effectiveness of these silk porous biomaterials in a larger, more complex biological model.
  • The researchers chose an adult equine (horse) model to evaluate the application of these biomaterials for soft-tissue regeneration over an extended period (6 months).
  • Both slow and rapidly degrading silk matrices were examined in two ways: in the subcutaneous pocket (under the skin) and in an intramuscular defect depot (within the muscle tissue).

Findings of the Study

  • The results indicate that the equine model effectively allows the simultaneous evaluation of multiple implants, thereby reducing the need for a large number of animal subjects.
  • The researchers discovered that they could control the speed of matrix degradation (the breakdown of the implanted silk) by altering the initial format of the implanted silk.
  • They also identified ultrasound imaging as a successful means of monitoring tissue regeneration and the degradation of the implant. The potential to visually track these processes is a significant advancement in the study and application of soft-tissue regeneration methods.”

Cite This Article

APA
Bellas E, Rollins A, Moreau JE, Lo T, Quinn KP, Fourligas N, Georgakoudi I, Leisk GG, Mazan M, Thane KE, Taeymans O, Hoffman AM, Kaplan DL, Kirker-Head CA. (2014). Equine model for soft-tissue regeneration. J Biomed Mater Res B Appl Biomater, 103(6), 1217-1227. https://doi.org/10.1002/jbm.b.33299

Publication

Journal of biomedical materials research. Part B, Applied biomaterials
ISSN: 1552-4981
NlmUniqueID: 101234238
Country: United States
Language: English
Volume: 103
Issue: 6
Pages: 1217-1227

Researcher Affiliations

Bellas, E
  • Department of Biomedical Engineering, Tufts University, Medford, MA.
Rollins, A
  • Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.
Moreau, J E
  • Department of Biomedical Engineering, Tufts University, Medford, MA.
Lo, T
  • Department of Mechanical Engineering, Tufts University, Medford, MA.
Quinn, K P
  • Department of Biomedical Engineering, Tufts University, Medford, MA.
Fourligas, N
  • Department of Biomedical Engineering, Tufts University, Medford, MA.
Georgakoudi, I
  • Department of Biomedical Engineering, Tufts University, Medford, MA.
Leisk, G G
  • Department of Mechanical Engineering, Tufts University, Medford, MA.
Mazan, M
  • Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.
Thane, K E
  • Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.
Taeymans, O
  • Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.
Hoffman, A M
  • Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.
Kaplan, D L
  • Department of Biomedical Engineering, Tufts University, Medford, MA.
Kirker-Head, C A
  • Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.

MeSH Terms

  • Absorbable Implants
  • Animals
  • Disease Models, Animal
  • Horses
  • Muscle, Skeletal / diagnostic imaging
  • Muscle, Skeletal / injuries
  • Muscle, Skeletal / physiology
  • Regeneration
  • Silk / chemistry
  • Ultrasonography

Grant Funding

  • F32 AR061933 / NIAMS NIH HHS
  • R01EB007542 / NIBIB NIH HHS
  • R01 EB007542 / NIBIB NIH HHS
  • P41 EB002520 / NIBIB NIH HHS
  • F32AR061933 / NIAMS NIH HHS
  • T32 EB005583 / NIBIB NIH HHS

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Citations

This article has been cited 4 times.
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  2. Leiva-Cepas F, Benito-Ysamat A, Jimena I, Jimenez-Diaz F, Gil-Belmonte MJ, Ruz-Caracuel I, Villalba R, Peña-Amaro J. Ultrasonographic and Histological Correlation after Experimental Reconstruction of a Volumetric Muscle Loss Injury with Adipose Tissue.. Int J Mol Sci 2021 Jun 22;22(13).
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