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Home / Equine Research Bank / Sci Rep / Equine odontoclastic tooth resorption and hypercementosis (E...
Scientific reports2023; 13(1); 5048; doi: 10.1038/s41598-023-32016-6

Equine odontoclastic tooth resorption and hypercementosis (EOTRH): microspatial distribution of trace elements in hypercementosis-affected and unaffected hard dental tissues.

Abstract: Equine Odontoclastic Tooth Resorption and Hypercementosis (EOTRH) is a common, painful and poorly understood disease. Enamel, dentin and cementum accumulate both essential and toxic trace elements during mineralization. Characterization of the spatial accumulation pattern of trace elements may provide insight into the role that toxic elements play and inform biological processes affecting these hard dental tissues for future research. Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was used to map the distribution of multiple trace elements and heavy metals across equine healthy and diseased (hypercementosis-affected) hard dental tissues among four teeth extracted from horses with EOTRH. Results showed banding patterns of some trace elements (lead, strontium, barium), reflecting the temporal component of accumulation of trace elements during dentin mineralization. Essential elements zinc and magnesium did not show banding patterns. Comparison to the unaffected cementum and dentin adjacent to the hypercementosis region showed that there is an underlying incremental pattern in the uptake of some metals with spatial irregularities. This supports a possible metabolic change involved in hypercementosis lesion development. This represents the first use of LA-ICP-MS to study the microspatial distribution of trace elements in equine teeth, establishing a baseline for elemental distribution in normal and EOTRH impacted dental hard tissue.
© 2023. The Author(s).
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Publication Date: 2023-03-28 PubMed ID: 36977746PubMed Central: PMC10050172DOI: 10.1038/s41598-023-32016-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the buildup of trace elements in horse teeth suffering from a disease, Equine Odontoclastic Tooth Resorption and Hypercementosis (EOTRH), using a specialized technique, Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The findings contribute to understanding the biological processes affecting hard dental tissues in horses and may pave the way for future research in various dental diseases.

Understanding the Research

This study focused on EOTRH, a prevalent yet not fully comprehended disease prevalent in horses that can cause considerable discomfort. The disease involves:

  • The hard dental tissues including enamel, dentin, and cementum.
  • The build-up of both essential and harmful trace elements during the mineralization process.

This research aimed to observe and characterise the pattern of this trace element accumulation, enabling an understanding of the role potentially harmful elements may play.

Methodology

The researchers utilized Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS), a sensitive analytical technique used to detect and map the concentrations of trace elements and heavy metals. They applied this technique to analyse both healthy and disease-affected (hypercementosis-affected) hard dental tissues from four teeth extracted from horses identified as EOTRH sufferers.

Results and Findings

  • The spatial distribution analysis of trace elements revealed patterns of banding for some elements like lead, strontium, and barium, suggesting a temporal element in the traces’ accumulation during dentin mineralization.
  • No banding patterns were evident for essential elements such as zinc and magnesium.
  • A comparative analysis of unaffected dentin and cementum near the hypercementosis region revealed an incremental pattern in some metals’ adjustment with clear spatial irregularities observed, suggesting a possible metabolic change during the hypercementosis lesion’s development.
  • This study’s novel aspect was that it is the first application of LA-ICP-MS to study the trace elements’ microspatial distribution in equine teeth. It established a basis on which future research can extract the elemental distribution pattern in healthy dental tissue and those affected by EOTRH.

Contribution and Implications for Future Research

The findings of this study could have important implications in understanding the pathophysiology of EOTRH and may contribute to future studies on the disease. Additionally, the use of LA-ICP-MS could potentially be applied to other dental diseases, leading to increased understanding of the disease process and possibly to the development of new prevention or treatment methods.

Cite This Article

APA
Wright AL, Earley ET, Austin C, Arora M. (2023). Equine odontoclastic tooth resorption and hypercementosis (EOTRH): microspatial distribution of trace elements in hypercementosis-affected and unaffected hard dental tissues. Sci Rep, 13(1), 5048. https://doi.org/10.1038/s41598-023-32016-6

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 5048
PII: 5048

Researcher Affiliations

Wright, Alexandra L
  • Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, 930 Campus Rd, Ithaca, NY, 14850, USA. aw656@cornell.edu.
Earley, Edward T
  • Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, 930 Campus Rd, Ithaca, NY, 14850, USA.
Austin, Christine
  • Environmental Medicine and Public Health, Icahn School of Medicine at Mount Sinai, 1 Gustave L Levy Place, New York, NY, 10029, USA.
  • Institute for Exposomic Research, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Arora, Manish
  • Environmental Medicine and Public Health, Icahn School of Medicine at Mount Sinai, 1 Gustave L Levy Place, New York, NY, 10029, USA.
  • Institute for Exposomic Research, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

MeSH Terms

  • Animals
  • Horses
  • Trace Elements
  • Hypercementosis / pathology
  • Hypercementosis / veterinary
  • Horse Diseases / pathology
  • Magnesium
  • Tooth Resorption / pathology

Grant Funding

  • R35 ES030435 / NIEHS NIH HHS
  • P30 ES023515 / NIEHS NIH HHS

Conflict of Interest Statement

The authors declare no competing interests.

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Citations

This article has been cited 2 times.
  1. Wright AL, Fiani N, Peralta S, Arora M, Austin C. Microspatial distribution of trace elements in feline dental hard tissues: early life exposure to essential and toxic elements. Front Vet Sci 2023;10:1204210.
    doi: 10.3389/fvets.2023.1204210pubmed: 37441556google scholar: lookup
  2. Jensen A, Clarke EJ, Nugent Z, Paice E, Gringel I, Yamamoto K, Rocchigiani G, Peffers AJ, Cooper L, Peffers MJ. Inflammation and response to bacterial infection as potential drivers of equine odontoclastic tooth resorption and hypercementosis: A proteomics insight. Equine Vet J 2025 Jul;57(4):977-990.
    doi: 10.1111/evj.14469pubmed: 39777419google scholar: lookup
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