Equine papillomavirus type 1: complete nucleotide sequence and characterization of recombinant virus-like particles composed of the EcPV-1 L1 major capsid protein.
Abstract: Equus caballus papillomavirus type 1 (EcPV-1) was isolated from a cutaneous papilloma, the most common neoplasm in horses. The complete EcPV-1 nucleotide sequence and genomic organization were determined. Phylogenetic analysis showed that EcPV-1 is a close-to-root papillomavirus, with only distant relationships to the fibropapillomaviruses and the benign cutaneous papillomaviruses. To produce EcPV-1 virus-like particles (VLPs), the EcPV-1 L1 major capsid protein was expressed in insect cells using a recombinant baculovirus vector. The self-assembled EcPV-1 VLPs were morphologically indistinguishable from wild type papillomavirus virions. Monoclonal antibodies were developed against intact and denatured EcPV-1 VLPs. When tested by ELISA, all monoclonal antibodies produced against intact (#18) and some against denatured EcPV-1 VLPs (#16) reacted with intact EcPV-1 VLPs only, demonstrating that the VLPs carry type-specific conformational as well as linear epitopes on their surface. Recombinant EcPV-1 VLPs offer the potential of a noninfectious vaccine to prevent and eradicate equine cutaneous papillomatosis.
Publication Date: 2004-10-16 PubMed ID: 15485669DOI: 10.1016/j.bbrc.2004.09.154Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article discusses the detailed examination and sequencing of Equus caballus papillomavirus type 1 (EcPV-1), a type of equine papillomavirus. The study also evaluates the potential of virus-like particles (VLPs) composed of EcPV-1’s major capsid protein to act as a noninfectious vaccine candidate for equine cutaneous papillomatosis.
Sequencing and Analysis of EcPV-1
- The isolation of EcPV-1 was carried out from a horse’s cutaneous papilloma, which is a common type of tumor observed in horses.
- Through analyzing the complete nucleotide sequence and genomic structure of the virus, the researchers were able to establish its phylogenetic position. In evolution terms, EcPV-1 is a fundamental papillomavirus type having distant relationships to fibropapillomaviruses and benign cutaneous papillomaviruses.
Production of EcPV-1 Virus-Like Particles (VLPs)
- For the production of VLPs, the researchers employed a technique involving the expression of EcPV-1’s L1 major capsid protein in insect cells via a baculovirus vector. This vector encourages the cells to produce the protein.
- The resultant VLPs were structurally identical to wild type papillomavirus virions, demonstrating their capacity to self-assemble into functional virus-like structures in vitro.
Monoclonal Antibodies and VLPs
- Monoclonal antibodies, produced specifically against these EcPV-1 VLPs, were also studied. These antibodies were developed against both intact and denatured VLPs.
- ELISA testing confirmed that these antibodies were successful in identifying intact EcPV-1 VLPs, suggesting they contains type-specific conformational and linear epitopes or specific binding sites on their surface, crucial for immune response.
Potential for Vaccine Development
- Given that the produced VLPs were equivalent to the wild type virus, and that the developed antibodies were successful in identifying these, the study concludes with the potential of these VLPs to be used as noninfectious vaccines.
- If further validated and developed, such a vaccine could effectively prevent or eradicate equine cutaneous papillomatosis in horses, having a significant impact on equine health.
Cite This Article
APA
Ghim SJ, Rector A, Delius H, Sundberg JP, Jenson AB, Van Ranst M.
(2004).
Equine papillomavirus type 1: complete nucleotide sequence and characterization of recombinant virus-like particles composed of the EcPV-1 L1 major capsid protein.
Biochem Biophys Res Commun, 324(3), 1108-1115.
https://doi.org/10.1016/j.bbrc.2004.09.154 Publication
Researcher Affiliations
- Laboratory of Vaccinology, John Graham Brown Cancer Center, University of Louisville Health Science Center, Louisville, KY, USA.
MeSH Terms
- Amino Acid Motifs
- Animals
- Antibodies, Monoclonal / chemistry
- Baculoviridae / metabolism
- Capsid Proteins / chemistry
- Capsid Proteins / genetics
- Cell Line
- DNA / chemistry
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Epitopes / chemistry
- Genome, Viral
- Horses
- Immunoblotting
- Insecta
- Mice
- Mice, Inbred BALB C
- Oncogene Proteins, Viral / chemistry
- Open Reading Frames
- Papillomaviridae / genetics
- Phylogeny
- Protein Conformation
- Recombinant Proteins / chemistry
- Recombination, Genetic
- Sequence Analysis, DNA
- Virion
Citations
This article has been cited 12 times.- Li Y, Zhang X, Zhao C, Lei X, Huang H, Shi Y, Li C, Bi J, Sun W, Lan T, Zheng M. Genetic characterization of Sus scrofa papillomavirus type 1 from domestic pigs in Guangxi Province, China. Braz J Microbiol 2023 Sep;54(3):2437-2443.
- Munday JS, Hardcastle MR, Sim M. Detection of a Putative Novel Papillomavirus Type within a Large Exophytic Papilloma on the Fetlock of a Horse. Pathogens 2020 Oct 5;9(10).
- Mira J, Herman M, Zakia LS, Olivo G, Araújo JP Jr, Borges AS, Oliveira-Filho JP. Frequency of Equus caballus papillomavirus in equine aural plaques. J Vet Diagn Invest 2018 Jul;30(4):565-568.
- Dong J, Zhu W, Yamashita N, Chambers JK, Uchida K, Kuwano A, Haga T. Isolation of equine papillomavirus type 1 from racing horse in Japan. J Vet Med Sci 2017 Dec 6;79(12):1957-1959.
- Bernard HU, Burk RD, Chen Z, van Doorslaer K, zur Hausen H, de Villiers EM. Classification of papillomaviruses (PVs) based on 189 PV types and proposal of taxonomic amendments. Virology 2010 May 25;401(1):70-9.
- Li L, Barry P, Yeh E, Glaser C, Schnurr D, Delwart E. Identification of a novel human gammapapillomavirus species. J Gen Virol 2009 Oct;90(Pt 10):2413-2417.
- Rector A, Lemey P, Tachezy R, Mostmans S, Ghim SJ, Van Doorslaer K, Roelke M, Bush M, Montali RJ, Joslin J, Burk RD, Jenson AB, Sundberg JP, Shapiro B, Van Ranst M. Ancient papillomavirus-host co-speciation in Felidae. Genome Biol 2007;8(4):R57.
- Postey RC, Appleyard GD, Kidney BA. Evaluation of equine papillomas, aural plaques, and sarcoids for the presence of Equine papillomavirus DNA and Papillomavirus antigen. Can J Vet Res 2007 Jan;71(1):28-33.
- Bravo IG, Alonso A. Phylogeny and evolution of papillomaviruses based on the E1 and E2 proteins. Virus Genes 2007 Jun;34(3):249-62.
- Al-Hammadi MA. First report on equine papillomavirus type 1 in Arabian horses in Saudi Arabia: Clinical, histopathological, and molecular characterization. Open Vet J 2025 Apr;15(4):1798-1802.
- Rodrigues GG, Alves DS, Bromberger CR, Nomelini QSS, Borges AS, de Oliveira-Filho JP, Delfiol DJZ. Clinical and epidemiological aspects in equine aural plaques. Vet Res Commun 2024 Nov 13;49(1):1.
- Munday JS, Knight CG, Luff JA. Papillomaviral skin diseases of humans, dogs, cats and horses: A comparative review. Part 1: Papillomavirus biology and hyperplastic lesions. Vet J 2022 Oct;288:105897.
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