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Frontiers in veterinary science2020; 7; 121; doi: 10.3389/fvets.2020.00121

Equine Parvovirus-Hepatitis in China: Characterization of Its Genetic Diversity and Evidence for Natural Recombination Events Between the Chinese and American Strains.

Abstract: Equine parvovirus-hepatitis (EqPV-H) was first reported in a horse that died of equine serum hepatitis in the USA in 2018, and was determined having a strong association with equine serum hepatitis in the following studies. As a newly discovered virus, the genomic sequences of only seven EqPV-H strains have been reported. Considering this, an epidemiological study was performed to investigate the prevalence of EqPV-H in equines in Guangdong Province in China, and obtain genomic sequences of the field prevalent EqPV-H strains. The detection rate of EqPV-H was finally determined to be 8.33% (95% CI: 2.8-18.4%), and EqPV-H's coinfection with equine hepacivirus and equine pegivirus was also determined. Then, the genomes of the Chinese field EqPV-H strains were obtained by PCR, sequencing, and assembly. Through bootscanning analysis, Simplot analysis, and phylogenetic analysis, strong evidence for natural recombination events were found in two Chinese field EqPV-H strains. The natural recombination events occurred between the Chinese and American strains, and were determined within VP protein. Finally, the genetic distance of EqPV-H strains was investigated. Nucleotide identities of 97.1-99.9% and 95.2-100% were found for NS and VP between EqPV-H strains, respectively. Together with other molecular evidence obtained in the present study, the genetic diversity of EqPV-H was determined. Taken together, the results of the present study expand our knowledge on the epidemiological characteristics, genetic variability, and evolution of EqPV-H.
Copyright © 2020 Lu, Wu, Ou and Li.
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Publication Date: 2020-03-10 PubMed ID: 32211433PubMed Central: PMC7076910DOI: 10.3389/fvets.2020.00121Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the prevalence of Equine parvovirus-hepatitis (EqPV-H) in horse populations in Guangdong Province in China, identifies the genetic sequencing of the prevalent strains, and establishes the genetic diversity of this recently discovered virus. The research identifies the occurrence of natural recombination events between the Chinese and American strains of EqPV-H.

Study Design and Findings

  • The researchers conducted an epidemiological study to identify the prevalence of EqPV-H in equines in Guangdong Province, China.
  • The final detection rate of EqPV-H was determined to be 8.33%.
  • The investigation also identified cases of coinfection with equine hepacivirus and equine pegivirus alongside EqPV-H.
  • The genomics of the prevalent EqPV-H strains in the field were obtained through PCR, sequencing, and assembly for detailed analysis.

Evidence of Natural Recombination

  • The genome sequences were analysed with bootscanning analysis, Simplot analysis and phylogenetic analysis techniques.
  • These analyses provided strong evidence of natural recombination events occurring in two field strains of EqPV-H in China.
  • The location of these recombination events was identified within the VP protein component of the virus, and between the Chinese and American strains of the virus.

Assessment of Genetic Diversity

  • The genetic distance of the studied EqPV-H strains was investigated to evaluate their genetic diversity.
  • The investigation found nucleotide identities of 97.1-99.9% and 95.2-100% for the NS and VP components of virus strains, respectively.
  • Bearing in mind this evidence and other molecular findings from the study, the researchers determined the genetic diversity of the EqPV-H.

Contribution to Understanding EqPV-H

  • The results of this study expand our understanding of the epidemiological characteristics, genetic variability and evolution of EqPV-H.
  • The finding of recombination events further highlight the complexity of the virus’s evolutionary pathway.

Cite This Article

APA
Lu G, Wu L, Ou J, Li S. (2020). Equine Parvovirus-Hepatitis in China: Characterization of Its Genetic Diversity and Evidence for Natural Recombination Events Between the Chinese and American Strains. Front Vet Sci, 7, 121. https://doi.org/10.3389/fvets.2020.00121

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 7
Pages: 121
PII: 121

Researcher Affiliations

Lu, Gang
  • College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
  • Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China.
  • Guangdong Technological Engineering Research Center for Pet, Guangzhou, China.
  • Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
Wu, Liyan
  • College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
  • Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China.
  • Guangdong Technological Engineering Research Center for Pet, Guangzhou, China.
  • Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
Ou, Jiajun
  • College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
  • Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China.
  • Guangdong Technological Engineering Research Center for Pet, Guangzhou, China.
  • Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
Li, Shoujun
  • College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
  • Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China.
  • Guangdong Technological Engineering Research Center for Pet, Guangzhou, China.
  • Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.

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Citations

This article has been cited 18 times.
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