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Stem cells (Dayton, Ohio)2006; 24(6); 1613-1619; doi: 10.1634/stemcells.2005-0264

Equine peripheral blood-derived progenitors in comparison to bone marrow-derived mesenchymal stem cells.

Abstract: Fibroblast-like cells isolated from peripheral blood of human, canine, guinea pig, and rat have been demonstrated to possess the capacity to differentiate into several mesenchymal lineages. The aim of this work was to investigate the possibility of isolating pluripotent precursor cells from equine peripheral blood and compare them with equine bone marrow-derived mesenchymal stem cells. Human mesenchymal stem cells (MSCs) were used as a control for cell multipotency assessment. Venous blood (n = 33) and bone marrow (n = 5) were obtained from adult horses. Mononuclear cells were obtained by Ficoll gradient centrifugation and cultured in monolayer, and adherent fibroblast-like cells were tested for their differentiation potential. Chondrogenic differentiation was performed in serum-free medium in pellet cultures as a three-dimensional model, whereas osteogenic and adipogenic differentiation were induced in monolayer culture. Evidence for differentiation was made via biochemical, histological, and reverse transcription-polymerase chain reaction evaluations. Fibroblast-like cells were observed on day 10 in 12 out of 33 samples and were allowed to proliferate until confluence. Equine peripheral blood-derived cells had osteogenic and adipogenic differentiation capacities comparable to cells derived from bone marrow. Both cell types showed a limited capacity to produce lipid droplets compared to human MSCs. This result may be due to the assay conditions, which are established for human MSCs from bone marrow and may not be optimal for equine progenitor cells. Bone marrow-derived equine and human MSCs could be induced to develop cartilage, whereas equine peripheral blood progenitors did not show any capacity to produce cartilage at the histological level. In conclusion, equine peripheral blood-derived fibroblast-like cells can differentiate into distinct mesenchymal lineages but have less multipotency than bone marrow-derived MSCs under the conditions used in this study.
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Publication Date: 2006-06-14 PubMed ID: 16769763DOI: 10.1634/stemcells.2005-0264Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study aims to determine the ability of precursor cells isolated from horse peripheral blood to differentiate into different cell types and to compare this ability with that of horse bone marrow-derived stem cells. The results showed that these blood-derived cells have similar resiliency to become other cell types as those taken from bone marrow. However, they are less efficient at producing certain types of tissue, such as cartilage, under specific conditions.

Research Methodology

  • The researchers collected venous blood and bone marrow samples from adult horses. They then isolated mononuclear cells from these samples using Ficoll gradient centrifugation, a technique used to separate different cell types based on their density.
  • These cells were cultured in a monolayer culture, allowing the adherent fibroblast-like cells to be examined for their potential to differentiate into different cell types.
  • Three different methods were used to cultivate different cell types: chondrogenic differentiation was carried out in serum-free medium as a three-dimensional model, and osteogenic and adipogenic differentiation were stimulated in monolayer culture.
  • Cell differentiation was confirmed through biochemical, histological, and reverse transcription-polymerase chain reaction evaluations.

Research Findings

  • Fibroblast-like cells were observed in 12 out of 33 samples by day 10, and these cells were allowed to grow until they covered the surface of the culture dish (reached confluence).
  • Peripheral blood-derived cells demonstrated osteogenic and adipogenic differentiation capacities that were similar to those of bone marrow-derived cells.
  • Both types of cells showed a limited ability to form lipid droplets compared to human mesenchymal stem cells, a phenomenon that could be attributed to the culture conditions, which were optimized for human cells.
  • While both bone marrow-derived horse and human stem cells were able to form cartilage, peripheral blood progenitors from horses did not possess this ability.

Conclusion

  • This study concluded that while horse peripheral blood-derived fibroblast-like cells can differentiate into distinct mesenchymal lineages (bone, fat), their versatility (or multipotency) is lower than that of bone marrow-derived stem cells, especially under the specific conditions used in this study.

Cite This Article

APA
Koerner J, Nesic D, Romero JD, Brehm W, Mainil-Varlet P, Grogan SP. (2006). Equine peripheral blood-derived progenitors in comparison to bone marrow-derived mesenchymal stem cells. Stem Cells, 24(6), 1613-1619. https://doi.org/10.1634/stemcells.2005-0264

Publication

Stem cells (Dayton, Ohio)
ISSN: 1066-5099
NlmUniqueID: 9304532
Country: England
Language: English
Volume: 24
Issue: 6
Pages: 1613-1619

Researcher Affiliations

Koerner, Jens
  • Institute of Pathology, Tissue Engineering Unit, University of Bern, Switzerland.
Nesic, Dobrila
    Romero, Jose Diaz
      Brehm, Walter
        Mainil-Varlet, Pierre
          Grogan, Shawn Patrick

            MeSH Terms

            • Adipogenesis
            • Animals
            • Base Sequence
            • Cell Differentiation
            • Cells, Cultured
            • Chondrogenesis
            • DNA, Complementary / genetics
            • Gene Expression
            • Hematopoietic Stem Cells / cytology
            • Hematopoietic Stem Cells / metabolism
            • Horses / anatomy & histology
            • Horses / blood
            • Horses / metabolism
            • Humans
            • In Vitro Techniques
            • Mesenchymal Stem Cells / cytology
            • Mesenchymal Stem Cells / metabolism
            • Multipotent Stem Cells / cytology
            • Multipotent Stem Cells / metabolism
            • Osteogenesis
            • Pluripotent Stem Cells / cytology
            • Pluripotent Stem Cells / metabolism
            • Species Specificity

            Citations

            This article has been cited 41 times.
            1. Petrova V, Vachkova E. Outlook of Adipose-Derived Stem Cells: Challenges to Their Clinical Application in Horses. Vet Sci 2023 May 12;10(5).
              doi: 10.3390/vetsci10050348pubmed: 37235430google scholar: lookup
            2. Stage HJ, Trappe S, Söllig K, Trachsel DS, Kirsch K, Zieger C, Merle R, Aschenbach JR, Gehlen H. Multilineage Differentiation Potential of Equine Adipose-Derived Stromal/Stem Cells from Different Sources. Animals (Basel) 2023 Apr 15;13(8).
              doi: 10.3390/ani13081352pubmed: 37106915google scholar: lookup
            3. El-Husseiny HM, Mady EA, Helal MAY, Tanaka R. The Pivotal Role of Stem Cells in Veterinary Regenerative Medicine and Tissue Engineering. Vet Sci 2022 Nov 21;9(11).
              doi: 10.3390/vetsci9110648pubmed: 36423096google scholar: lookup
            4. Shirzeyli MH, Tayyebiazar A, Aliakbari F, Ghasemi F, Eini F, Shirzeyli FH, Vanaki E, Sobhani A. Comparison of the efficacy of bone morphogenetic protein-4 on in vitro differentiation of murine adipose and bone marrow mesenchymal stem cells into primordial germ cells. Res Pharm Sci 2022 Apr;17(2):123-133.
              doi: 10.4103/1735-5362.335171pubmed: 35280837google scholar: lookup
            5. Ma Y, Zhou D, Zhang H, Tang L, Qian F, Su J. Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote the Proliferation of Schwann Cells by Regulating the PI3K/AKT Signaling Pathway via Transferring miR-21. Stem Cells Int 2021;2021:1496101.
              doi: 10.1155/2021/1496101pubmed: 34552631google scholar: lookup
            6. Prządka P, Buczak K, Frejlich E, Gąsior L, Suliga K, Kiełbowicz Z. The Role of Mesenchymal Stem Cells (MSCs) in Veterinary Medicine and Their Use in Musculoskeletal Disorders. Biomolecules 2021 Aug 2;11(8).
              doi: 10.3390/biom11081141pubmed: 34439807google scholar: lookup
            7. Bertoni L, Jacquet-Guibon S, Branly T, Desancé M, Legendre F, Melin M, Rivory P, Hartmann DJ, Schmutz A, Denoix JM, Demoor M, Audigié F, Galéra P. Evaluation of Allogeneic Bone-Marrow-Derived and Umbilical Cord Blood-Derived Mesenchymal Stem Cells to Prevent the Development of Osteoarthritis in An Equine Model. Int J Mol Sci 2021 Mar 2;22(5).
              doi: 10.3390/ijms22052499pubmed: 33801461google scholar: lookup
            8. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals (Basel) 2021 Jan 19;11(1).
              doi: 10.3390/ani11010234pubmed: 33477808google scholar: lookup
            9. Bashiri Dezfouli A, Pourfathollah AA, Nikougoftar-Zarif M, Khosravi M, Tajrishi M, Ezzati N, Kashani Khatib Z, Abbasi Sourki P, Valizadeh M. Optimizing the recovery of peripheral blood mononuclear cells trapped in leukoreduction filters - A comparison study. Hematol Transfus Cell Ther 2022 Apr-Jun;44(2):197-205.
              doi: 10.1016/j.htct.2020.09.155pubmed: 33423981google scholar: lookup
            10. Kim SA, Park HY, Shin YW, Go EJ, Kim YJ, Kim YC, Shetty AA, Kim SJ. Hemovac blood after total knee arthroplasty as a source of stem cells. Ann Transl Med 2020 Nov;8(21):1406.
              doi: 10.21037/atm-20-2215pubmed: 33313151google scholar: lookup
            11. Mocchi M, Dotti S, Bue MD, Villa R, Bari E, Perteghella S, Torre ML, Grolli S. Veterinary Regenerative Medicine for Musculoskeletal Disorders: Can Mesenchymal Stem/Stromal Cells and Their Secretome Be the New Frontier?. Cells 2020 Jun 11;9(6).
              doi: 10.3390/cells9061453pubmed: 32545382google scholar: lookup
            12. Lotfy A, El-Sherbiny YM, Cuthbert R, Jones E, Badawy A. Comparative study of biological characteristics of mesenchymal stem cells isolated from mouse bone marrow and peripheral blood. Biomed Rep 2019 Oct;11(4):165-170.
              doi: 10.3892/br.2019.1236pubmed: 31565222google scholar: lookup
            13. Yang Q, Pinto VMR, Duan W, Paxton EE, Dessauer JH, Ryan W, Lopez MJ. In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates. Front Bioeng Biotechnol 2019;7:155.
              doi: 10.3389/fbioe.2019.00155pubmed: 31355191google scholar: lookup
            14. Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance. Cell Transplant 2018 Jan;27(1):93-116.
              doi: 10.1177/0963689717724797pubmed: 29562773google scholar: lookup
            15. Olivera R, Moro LN, Jordan R, Pallarols N, Guglielminetti A, Luzzani C, Miriuka SG, Vichera G. Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses. Stem Cells Cloning 2018;11:13-22.
              doi: 10.2147/SCCAA.S151763pubmed: 29497320google scholar: lookup
            16. Ishikawa S, Horinouchi C, Mizoguchi R, Senokuchi A, Kamikakimoto R, Murata D, Hatazoe T, Tozaki T, Misumi K, Hobo S. Isolation of equine peripheral blood stem cells from a Japanese native horse. J Equine Sci 2017;28(4):153-158.
              doi: 10.1294/jes.28.153pubmed: 29270073google scholar: lookup
            17. Patruno M, Gomiero C, Sacchetto R, Topel O, Negro A, Martinello T. Tat-MyoD fused proteins, together with C2c12 conditioned medium, are able to induce equine adult mesenchimal stem cells towards the myogenic fate. Vet Res Commun 2017 Sep;41(3):211-217.
              doi: 10.1007/s11259-017-9692-ypubmed: 28589421google scholar: lookup
            18. Olivera R, Moro LN, Jordan R, Luzzani C, Miriuka S, Radrizzani M, Donadeu FX, Vichera G. In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors. PLoS One 2016;11(10):e0164049.
              doi: 10.1371/journal.pone.0164049pubmed: 27732616google scholar: lookup
            19. Liu S, Zhou J, Zhang X, Liu Y, Chen J, Hu B, Song J, Zhang Y. Strategies to Optimize Adult Stem Cell Therapy for Tissue Regeneration. Int J Mol Sci 2016 Jun 21;17(6).
              doi: 10.3390/ijms17060982pubmed: 27338364google scholar: lookup
            20. Fülber J, Maria DA, da Silva LC, Massoco CO, Agreste F, Baccarin RY. Comparative study of equine mesenchymal stem cells from healthy and injured synovial tissues: an in vitro assessment. Stem Cell Res Ther 2016 Mar 5;7:35.
              doi: 10.1186/s13287-016-0294-3pubmed: 26944403google scholar: lookup
            21. Gomiero C, Bertolutti G, Martinello T, Van Bruaene N, Broeckx SY, Patruno M, Spaas JH. Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology. Vet Res Commun 2016 Mar;40(1):39-48.
              doi: 10.1007/s11259-016-9652-ypubmed: 26757735google scholar: lookup
            22. Wu G, Pan M, Wang X, Wen J, Cao S, Li Z, Li Y, Qian C, Liu Z, Wu W, Zhu L, Guo J. Osteogenesis of peripheral blood mesenchymal stem cells in self assembling peptide nanofiber for healing critical size calvarial bony defect. Sci Rep 2015 Nov 16;5:16681.
              doi: 10.1038/srep16681pubmed: 26568114google scholar: lookup
            23. Han X, Wu X, Liu H, Wang D, E L, Zhou W. Ectopic osteogenesis of an injectable nHAC/CSH loaded with blood-acquired mesenchymal progenitor cells in a nude mice model. J Mater Sci Mater Med 2015 Jan;26(1):5338.
              doi: 10.1007/s10856-014-5338-6pubmed: 25577218google scholar: lookup
            24. Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential. Stem Cell Res Ther 2014 Feb 21;5(1):25.
              doi: 10.1186/scrt414pubmed: 24559797google scholar: lookup
            25. De Schauwer C, Goossens K, Piepers S, Hoogewijs MK, Govaere JL, Smits K, Meyer E, Van Soom A, Van de Walle GR. Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources. Stem Cell Res Ther 2014 Jan 13;5(1):6.
              doi: 10.1186/scrt395pubmed: 24418262google scholar: lookup
            26. Guest DJ, Ousey JC, Smith MR. Defining the expression of marker genes in equine mesenchymal stromal cells. Stem Cells Cloning 2008;1:1-9.
              doi: 10.2147/sccaa.s3824pubmed: 24198500google scholar: lookup
            27. Kang JG, Park SB, Seo MS, Kim HS, Chae JS, Kang KS. Characterization and clinical application of mesenchymal stem cells from equine umbilical cord blood. J Vet Sci 2013;14(3):367-71.
              doi: 10.4142/jvs.2013.14.3.367pubmed: 23820166google scholar: lookup
            28. Tan SL, Ahmad TS, Selvaratnam L, Kamarul T. Isolation, characterization and the multi-lineage differentiation potential of rabbit bone marrow-derived mesenchymal stem cells. J Anat 2013 Apr;222(4):437-50.
              doi: 10.1111/joa.12032pubmed: 23510053google scholar: lookup
            29. Coli A, Nocchi F, Lamanna R, Iorio M, Lapi S, Urciuoli P, Scatena F, Giannessi E, Stornelli MR, Passeri S. Isolation and characterization of equine amnion mesenchymal stem cells. Cell Biol Int Rep (2010) 2011 Sep 13;18(1):e00011.
              doi: 10.1042/CBR20110004pubmed: 23124164google scholar: lookup
            30. Lyahyai J, Mediano DR, Ranera B, Sanz A, Remacha AR, Bolea R, Zaragoza P, Rodellar C, Martín-Burriel I. Isolation and characterization of ovine mesenchymal stem cells derived from peripheral blood. BMC Vet Res 2012 Sep 22;8:169.
              doi: 10.1186/1746-6148-8-169pubmed: 22999337google scholar: lookup
            31. Proffen BL, Haslauer CM, Harris CE, Murray MM. Mesenchymal stem cells from the retropatellar fat pad and peripheral blood stimulate ACL fibroblast migration, proliferation, and collagen gene expression. Connect Tissue Res 2013;54(1):14-21.
              doi: 10.3109/03008207.2012.715701pubmed: 22839298google scholar: lookup
            32. Kitada M, Wakao S, Dezawa M. Muse cells and induced pluripotent stem cell: implication of the elite model. Cell Mol Life Sci 2012 Nov;69(22):3739-50.
              doi: 10.1007/s00018-012-0994-5pubmed: 22527723google scholar: lookup
            33. Corradetti B, Lange-Consiglio A, Barucca M, Cremonesi F, Bizzaro D. Size-sieved subpopulations of mesenchymal stem cells from intervascular and perivascular equine umbilical cord matrix. Cell Prolif 2011 Aug;44(4):330-42.
              doi: 10.1111/j.1365-2184.2011.00759.xpubmed: 21645152google scholar: lookup
            34. Fite BZ, Decaris M, Sun Y, Sun Y, Lam A, Ho CK, Leach JK, Marcu L. Noninvasive multimodal evaluation of bioengineered cartilage constructs combining time-resolved fluorescence and ultrasound imaging. Tissue Eng Part C Methods 2011 Apr;17(4):495-504.
              doi: 10.1089/ten.tec.2010.0368pubmed: 21303258google scholar: lookup
            35. Wang X, Moutsoglou D. Osteogenic and adipogenic differentiation potential of an immortalized fibroblast-like cell line derived from porcine peripheral blood. In Vitro Cell Dev Biol Anim 2009 Dec;45(10):584-91.
              doi: 10.1007/s11626-009-9231-4pubmed: 19633899google scholar: lookup
            36. Colleoni S, Bottani E, Tessaro I, Mari G, Merlo B, Romagnoli N, Spadari A, Galli C, Lazzari G. Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor. Vet Res Commun 2009 Dec;33(8):811-21.
              doi: 10.1007/s11259-009-9229-0pubmed: 19472068google scholar: lookup
            37. Koch TG, Berg LC, Betts DH. Current and future regenerative medicine - principles, concepts, and therapeutic use of stem cell therapy and tissue engineering in equine medicine. Can Vet J 2009 Feb;50(2):155-65.
              pubmed: 19412395
            38. Vidal MA, Robinson SO, Lopez MJ, Paulsen DB, Borkhsenious O, Johnson JR, Moore RM, Gimble JM. Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow. Vet Surg 2008 Dec;37(8):713-24.
              doi: 10.1111/j.1532-950X.2008.00462.xpubmed: 19121166google scholar: lookup
            39. Koch TG, Heerkens T, Thomsen PD, Betts DH. Isolation of mesenchymal stem cells from equine umbilical cord blood. BMC Biotechnol 2007 May 30;7:26.
              doi: 10.1186/1472-6750-7-26pubmed: 17537254google scholar: lookup
            40. Garon EB, Marcu L, Luong Q, Tcherniantchouk O, Crooks GM, Koeffler HP. Quantum dot labeling and tracking of human leukemic, bone marrow and cord blood cells. Leuk Res 2007 May;31(5):643-51.
              doi: 10.1016/j.leukres.2006.08.006pubmed: 17027955google scholar: lookup
            41. Kim EY, Lee EJ, Kim RE, Kil TY, Kim MK. Evaluation of stability and safety of equine mesenchymal stem cells derived from amniotic fluid for clinical application. Front Vet Sci 2024;11:1330009.
              doi: 10.3389/fvets.2024.1330009pubmed: 38420210google scholar: lookup
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