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Stem cell research & therapy2013; 4(4); 85; doi: 10.1186/scrt236

Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: a randomized controlled trial.

Abstract: Tendon injury is a major cause of lameness and decreased performance in athletic equines. Various therapies for tendonitis have been described; however, none of these therapies results in complete tissue regeneration, and the injury recurrence rate is high even after long recovery periods involving rest and physiotherapy. Methods: A lesion was induced with collagenase gel in the superficial digital flexor tendon in the center portion of the metacarpal region of eight equines of mixed breed. After two weeks, the lesions of the animals in the treated and control groups were treated through the intralesional administration of mesenchymal stem cells derived from adipose tissue (adMSCs) suspended in platelet concentrate (PC) and with phosphate buffered saline (PBS), respectively. Serial ultrasound analyses were performed every two weeks. After 16 weeks of therapy, a biopsy was performed for histopathological, immunohistochemical and gene expression (type I collagen (COL1A1), type III collagen (COL3A1), tenascin-C (TNC), tenomodulin (TNMD), and scleraxis (SCX)) analyses. Results: Differences in the ultrasound and histopathological analyses were observed between the groups. Improved results were reported in the group treated with adMSCs suspended in PC. There was no difference in the gene expression levels observed after the different treatments. The main results observed from the histopathological evaluation of the treated group were as follows: a prevention of the progression of the lesion, a greater organization of collagen fibers, and a decreased inflammatory infiltrate. A lack of progression of the lesion area and its percentage was observed in the ultrasound image, and increased blood flow was measured by Power Doppler. Conclusions: The use of adMSCs combined with PC for the therapy of experimentally induced tendonitis prevented the progression of the tendon lesion, as observed in the ultrasound examination, and resulted in a greater organization and decreased inflammation, as observed in the histopathological evaluation. These data demonstrate the therapeutic potential of this therapy for the treatment of equine tendonitis.
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Publication Date: 2013-07-22 PubMed ID: 23876512PubMed Central: PMC3854756DOI: 10.1186/scrt236Google Scholar: Lookup
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  • Journal Article
  • Randomized Controlled Trial
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article provides an experimental study investigating the effectiveness and potential of using adipose-derived mesenchymal stem cells (adMSCs) and platelet concentrate (PC) in the treatment of tendonitis in equine subjects. The study concluded that this method is effective in preventing the progression of tendon injuries, reducing inflammation, and promoting better organization of collagen fibers.

Experimental Method and Procedure

The study involved eight mixed breed equines with artificially induced superficial digital flexor tendon lesions using a collagenase gel. After two weeks from the induction of the lesion, the researchers implemented the treatment – intralegional administration of adMSCs suspended in PC for the treated group and with phosphate buffered saline (PBS) for the control group. They conducted:

  • Periodic ultrasound analyses every two weeks.
  • A biopsy after 16 weeks for histopathological, immunohistochemical, and gene expression analyses which included type I collagen (COL1A1), type III collagen (COL3A1), tenascin-C (TNC), tenomodulin (TNMD), and scleraxis (SCX).

Major Findings

The results of both the treated and the control groups were compared and contrasted, showing significant differences. For the equines treated with adMSCs and PC:

  • There was a notable decrease in the progression of the tendon lesion.
  • Increased organization of collagen fibers was observed.
  • Inflammation was significantly reduced.
  • Ultrasound imaging demonstrated a lack of progression of the lesion area and its percentage.
  • Power Doppler readings showed increased blood flow.
  • No variation was found in the gene expression levels after the different treatments.

Conclusions

The study concluded that a combination of adMSCs and PC was successful in halting the progression of tendon injuries in equines, resulting in decreased inflammation and a better arrangement of collagen fibers. The implications of these results highlight the therapeutic potential of adMSCs and PC in treating equine tendonitis, although no significant changes were found in the gene expression levels of treated subjects. Overall, this experimental study contributes valuable insights and understanding towards the development of more effective treatments for equine tendon injuries.

Cite This Article

APA
Carvalho Ade M, Badial PR, Álvarez LE, Yamada AL, Borges AS, Deffune E, Hussni CA, Garcia Alves AL. (2013). Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: a randomized controlled trial. Stem Cell Res Ther, 4(4), 85. https://doi.org/10.1186/scrt236

Publication

Stem cell research & therapy
ISSN: 1757-6512
NlmUniqueID: 101527581
Country: England
Language: English
Volume: 4
Issue: 4
Pages: 85

Researcher Affiliations

Carvalho, Armando de Mattos
    Badial, Peres Ramos
      Álvarez, Luis Emiliano Cisneros
        Yamada, Ana Lucia Miluzzi
          Borges, Alexandre Secorun
            Deffune, Elenice
              Hussni, Carlos Alberto
                Garcia Alves, Ana Liz

                  MeSH Terms

                  • Animals
                  • Blood Platelets / metabolism
                  • Cell- and Tissue-Based Therapy / methods
                  • Female
                  • Horse Diseases / pathology
                  • Horses
                  • Male
                  • Mesenchymal Stem Cells / metabolism
                  • Tendon Injuries / blood
                  • Tendon Injuries / therapy

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