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Home / Equine Research Bank / Vet Med Int / Equine Trypanosomiasis: Molecular Detection, Hematological, ...
Veterinary medicine international2024; 2024; 6550276; doi: 10.1155/2024/6550276

Equine Trypanosomiasis: Molecular Detection, Hematological, and Oxidative Stress Profiling.

Abstract: Surra caused by () is widely distributed and has significant impact on equine sector and international trades. However, there are no available data about the genetic characterization of this parasite in horses in Egypt. So, the goal of this study was to study the molecular characterization of in horses and determine the changes in hematological parameters and oxidative stress associated with infection. A total of 12 horses were examined using PCR targeting RoTat 1.2 VSG gene, and we evaluated the changes in hematological and oxidative stress between infected and healthy animals. The results revealed a notable reduction in red blood cell (RBC), hematocrit (HCT), and hemoglobin (Hb) levels in the infected horses, as compared to the control healthy group. In contrast, the infected group showed a substantial increase in mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), and mean corpuscular volume (MCV). In addition, the infected group exhibited monocytopenia, eosinopenia, and notable lymphocytopenia. Regarding oxidative stress profile, the infected horses showed decreased levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and antioxidant capacity (TAC) compared to the control group. Moreover, the PCR assay targeting RoTat 1.2 VSG gene revealed positive specific band (205 bp) in all examined samples for and the phylogenetic analysis demonstrated that strain of this study is closely related to isolate in horses from India (MT501210) while showed difference from sequences of other species. The results emphasize the changes in blood composition and the body's response to oxidative stress caused by infection in horses.
Copyright © 2024 Mostafa Shoraba et al.
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Publication Date: 2024-08-16 PubMed ID: 39184948PubMed Central: PMC11343626DOI: 10.1155/2024/6550276Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the molecular characterization of Trypanosoma evansi (which causes surra disease) in horses in Egypt, examining the changes in blood parameters and oxidative stress in infected animals compared to healthy ones.

Objective and Methodology

  • The aim of the study was to characterize the parasite Trypanosoma evansi at a molecular level in Egyptian horses and to study the changes in hematological parameters and the oxidative stress profile associated with this infection.
  • A total of twelve horses were examined with PCR, a gene-targeting technique, focusing on the RoTat 1.2 VSG gene that is related to the infection.
  • Hematological and oxidative stress parameters were evaluated and compared between infected and healthy horses.

Results

  • The research found a significant reduction in red blood cell (RBC), hematocrit (HCT), and hemoglobin (Hb) levels in infected horses compared to the healthy control group.
  • Conversely, infected horses showed substantial increases in mean corpuscular hemoglobin concentratio (MCHC), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH).
  • Furthermore, infected horses exhibited symptoms such as monocytopenia, eosinopenia, and a noticeable decrease in lymphocytes (lymphocytopenia).
  • Regarding oxidative stress, infected horses showed lower levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and overall antioxidant capacity (TAC) than the control group.
  • The PCR assay targeting the RoTat 1.2 VSG gene showed a positive specific band in all examined samples, indicating the presence of Trypanosoma evansi. Phylogenetic analysis revealed that the identified strain is closely related to a strain found in Indian horses, with notable differences from sequences of other species.

Conclusion

  • The findings of the study highlight significant changes in the composition of the blood and the oxidative stress response caused by Trypanosoma evansi infections in horses.
  • This research contributes to a better understanding of the disease, providing valuable insights into the disease’s physiological effects which may be of utility for advancements in veterinary medicine and the equine industry.

Cite This Article

APA
Shoraba M, Shoulah SA, Arnaout F, Selim A. (2024). Equine Trypanosomiasis: Molecular Detection, Hematological, and Oxidative Stress Profiling. Vet Med Int, 2024, 6550276. https://doi.org/10.1155/2024/6550276

Publication

Veterinary medicine international
ISSN: 2090-8113
NlmUniqueID: 101524203
Country: United States
Language: English
Volume: 2024
Pages: 6550276
PII: 6550276

Researcher Affiliations

Shoraba, Mostafa
  • Department of Animal Medicine (Infectious Diseases) Faculty of Veterinary Medicine Benha University, Toukh 13736, Egypt.
Shoulah, Salma A
  • Department of Animal Medicine (Infectious Diseases) Faculty of Veterinary Medicine Benha University, Toukh 13736, Egypt.
Arnaout, Faysal
  • Department of Animal Medicine (Infectious Diseases) Faculty of Veterinary Medicine Benha University, Toukh 13736, Egypt.
Selim, Abdelfattah
  • Department of Animal Medicine (Infectious Diseases) Faculty of Veterinary Medicine Benha University, Toukh 13736, Egypt.

Conflict of Interest Statement

The authors declare that there are no conflicts of interest.

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