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Home / Equine Research Bank / Can J Vet Res / Equine vitreous humor chemical concentrations: correlation w...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire1988; 52(4); 476-480;

Equine vitreous humor chemical concentrations: correlation with serum concentrations, and postmortem changes with time and temperature.

Abstract: Samples of equine vitreous humor were analyzed for urea nitrogen, creatinine, sodium, chloride, phosphorus, calcium, potassium, and magnesium concentrations. Fresh vitreous chemical concentrations were compared to corresponding serum concentrations and expressed as a vitreous to serum ratio. The effects of postmortem time and temperature on the stability of the chemical concentrations were determined by incubation of the samples at 4 degrees C, and 20 degrees C and 37 degrees C for 6, 12, 24, or 48 h. Chemical concentrations were generally more stable at lower temperatures and shorter incubation times.
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Publication Date: 1988-10-01 PubMed ID: 3196976PubMed Central: PMC1255494
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines the chemical concentrations in the vitreous humor (clear gel that fills the eye) of horses, comparing these to the horse’s serum concentrations, and investigating how these concentrations change post mortem under different temperatures and time periods.

Chemical Concentrations Analysis

  • The study started with an analysis of the chemical samples extracted from the horse’s vitreous humor. The chemicals examined were common body metabolites and electrolytes including urea nitrogen, creatinine, sodium, chloride, phosphorus, calcium, potassium, an magnesium.
  • The purpose of this analysis was to build a baseline understanding of the constitution of chemicals in the vitreous humor which can contribute to various biological understandings and medical diagnoses.

Comparison with Serum Concentrations

  • The researchers then compared these concentrations to the levels found in the horse’s serum. This comparison was expressed as a vitreous to serum ratio.
  • The purpose of this step was to understand the relationship between the concentrations of these chemicals in the vitreous humor and serum. How these concentrations compare can provide insights into how these body fluids relate and influence each other.

Impact of Time and Temperature Postmortem

  • The study also examined how these concentrations change postmortem with the passage of time and varying temperatures. The researchers incubated the samples at three different temperatures: 4 degrees Celsius, 20 degrees Celsius, and 37 degrees Celsius, for periods of 6, 12, 24, or 48 hours.
  • The research’s findings were that chemical concentrations were generally more stable at lower temperatures and shorter incubation times.
  • This component of the research can aid in forensic investigations, as it helps understand how these chemicals react after death, which can give clues to the time and conditions of death.

Cite This Article

APA
McLaughlin BG, McLaughlin PS. (1988). Equine vitreous humor chemical concentrations: correlation with serum concentrations, and postmortem changes with time and temperature. Can J Vet Res, 52(4), 476-480.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 0830-9000
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 52
Issue: 4
Pages: 476-480

Researcher Affiliations

McLaughlin, B G
  • Department of Pathology, Murray State University Breathitt Veterinary Center, Hopkinsville, Kentucky 42240.
McLaughlin, P S

    MeSH Terms

    • Animals
    • Blood Urea Nitrogen
    • Calcium / analysis
    • Calcium / blood
    • Chlorides / analysis
    • Chlorides / blood
    • Creatinine / analysis
    • Creatinine / blood
    • Female
    • Horses / metabolism
    • Magnesium / analysis
    • Magnesium / blood
    • Male
    • Nitrogen / analysis
    • Phosphorus / analysis
    • Phosphorus / blood
    • Potassium / analysis
    • Potassium / blood
    • Sodium / analysis
    • Sodium / blood
    • Temperature
    • Time Factors
    • Urea / analysis
    • Vitreous Body / analysis

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    This article includes 22 references
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    Citations

    This article has been cited 6 times.
    1. Fingerhut L, Ohnesorge B, von Borstel M, Schumski A, Strutzberg-Minder K, Mörgelin M, Deeg CA, Haagsman HP, Beineke A, von Köckritz-Blickwede M, de Buhr N. Neutrophil Extracellular Traps in the Pathogenesis of Equine Recurrent Uveitis (ERU). Cells 2019 Nov 27;8(12).
      doi: 10.3390/cells8121528pubmed: 31783639google scholar: lookup
    2. Junginger J, Hansmann F, Herder V, Lehmbecker A, Peters M, Beyerbach M, Wohlsein P, Baumgärtner W. Pathology in Captive Wild Felids at German Zoological Gardens. PLoS One 2015;10(6):e0130573.
      doi: 10.1371/journal.pone.0130573pubmed: 26086731google scholar: lookup
    3. Hanna PE, Bellamy JE, Donald A. Postmortem eyefluid analysis in dogs, cats and cattle as an estimate of antemortem serum chemistry profiles. Can J Vet Res 1990 Oct;54(4):487-94.
      pubmed: 2249181
    4. Zorotrian T, Stern AW, Gao H, Costidis A, Fontaine C, Deming A, Harms C, Adams HR. Precision of the Abaxis VetScan VS2 for postmortem biochemical analysis of delphinid vitreous humor. Mar Mamm Sci 2023 Jul;39(3):893-905.
      doi: 10.1111/mms.13014pubmed: 39898359google scholar: lookup
    5. Zorotrian T, Stern AW, Costidis A, Harms CA, Gao H, Fontaine C, Adams HR. Delphinid postmortem vitreous humor biochemistry as proxy for antemortem blood analyte concentrations. Mar Mamm Sci 2025 Jan;41(1).
      doi: 10.1111/mms.13157pubmed: 39850025google scholar: lookup
    6. Palić M, Šoštarić Zuckermann IC, Džaja P, Ljubić BB, Severin K. A Biochemical and Histological Assessment of Postmortem Changes to the Eyes of Domestic Pigs: A Preliminary Study. Animals (Basel) 2024 Apr 15;14(8).
      doi: 10.3390/ani14081190pubmed: 38672338google scholar: lookup
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