Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses.
Abstract: In the present study, an equine-derived cell line was established by transfecting primary fetal horse kidney (FHK) cells with expression plasmid encoding simian virus 40 (SV40) large T antigen and then cloning them by limiting dilution. The cloned cell line, named FHK-Tcl3, grew well and could be propagated over 30 times by splitting them 1:3. Equine herpesvirus (EHV)-1 and EHV-4 replicated well in FHK-Tcl3. EHV-2 and EHV-4 were isolated from samples collected from horses in the field using FHK-Tcl3, and EHV-3 also propagated in FHK-Tcl3. These results indicated that this novel cell line, FHK-Tcl3, can be used for isolation and propagation of equine herpesviruses.
Publication Date: 2007-10-06 PubMed ID: 17917390DOI: 10.1292/jvms.69.989Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research focuses on the development of a new horse-derived cell line, created by modifying primary fetal horse kidney cells, which can be used to isolate and propagate equine herpesviruses (EHV).
Establishment of the Cell Line
- The researchers created a novel cell line from equine (horse-specific) origin. This was done by transfecting, or introducing, an expression plasmid encoding for the simian virus 40 (SV40) large T antigen into primary fetal horse kidney (FHK) cells.
- Through a method called limiting dilution, the cells were cloned, resulting in a new cell line that was named FHK-Tcl3.
Growth and Propagation of the Cell Line
- The newly established FHK-Tcl3 cell line showed good growth rate and could be propagated over 30 times by splitting them 1:3. This means that the cells could multiply and form new cells efficiently.
Role in Equine Herpesvirus Replication and Isolation
- The researchers found that Equine herpesvirus (EHV)-1 and EHV-4 replicated well in the FHK-Tcl3 cell line. This means that these specific viruses were able to use the cells to reproduce effectively.
- EHV-2 and EHV-4 were successfully isolated from samples collected from horses in the field using the FHK-Tcl3 cell line. This suggests that FHK-Tcl3 can be used as a medium for separating these specific equine herpesviruses from other microorganisms or compounds.
- Moreover, the team managed to propagate EHV-3 in the FHK-Tcl3 cell line, further reinforcing its potential use in virus isolation and propagation.
In conclusion, the FHK-Tcl3 cell line, established from fetal horse kidney cells using genetic engineering, has been shown to successfully isolate and propagate multiple variants of equine herpesvirus, highlighting its potential application in the veterinary virology field.
Cite This Article
APA
Maeda K, Yasumoto S, Tsuruda A, Andoh K, Kai K, Otoi T, Matsumura T.
(2007).
Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses.
J Vet Med Sci, 69(9), 989-991.
https://doi.org/10.1292/jvms.69.989 Publication
Researcher Affiliations
- Laboratory of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. kmaeda@yamaguchi-u.ac.jp
MeSH Terms
- Animals
- Cell Line / cytology
- Cell Line / virology
- Clone Cells
- Cytopathogenic Effect, Viral
- Embryo, Mammalian
- Herpesvirus 1, Equid / growth & development
- Herpesvirus 1, Equid / isolation & purification
- Herpesvirus 4, Equid / growth & development
- Herpesvirus 4, Equid / isolation & purification
- Horse Diseases / virology
- Horses
- Kidney / cytology
Citations
This article has been cited 11 times.- Faragher RGA. Simple Detection Methods for Senescent Cells: Opportunities and Challenges. Front Aging 2021;2:686382.
- Badr Y, Rahman MM, Ohno Y, Ishijima K, Maeda K, Kohyama K, Kamatari YO, Shimizu K, Okada A, Inoshima Y. A New Enzyme-linked Immunosorbent Assay for Serological Diagnosis of Seal Parapoxvirus Infection in Marine Mammals. J Vet Res 2022 Mar;66(1):43-52.
- Vissani MA, Damiani AM, Barrandeguy ME. Equine Coital Exanthema: New Insights on the Knowledge and Leading Perspectives for Treatment and Prevention. Pathogens 2021 Aug 20;10(8).
- Sakaguchi S, Koide R, Miyazawa T. In vitro host range of feline morbillivirus. J Vet Med Sci 2015 Nov;77(11):1485-7.
- Yun N, Lee YM, Kim C, Shibayama H, Tanimura A, Hamanaka Y, Kanakura Y, Park IS, Jo A, Shin JH, Ju C, Kim WK, Oh YJ. Anamorsin, a novel caspase-3 substrate in neurodegeneration. J Biol Chem 2014 Aug 8;289(32):22183-95.
- Andoh K, Kai K, Matsumura T, Maeda K. Further Development of an Equine Cell Line that can be Propagated over 100 Times. J Equine Sci 2009;20(2):11-4.
- Mahmoud HY, Andoh K, Hattori S, Terada Y, Noguchi K, Shimoda H, Maeda K. Characterization of glycoproteins in equine herpesvirus-1. J Vet Med Sci 2013 Oct;75(10):1317-21.
- Azab W, Zajic L, Osterrieder N. The role of glycoprotein H of equine herpesviruses 1 and 4 (EHV-1 and EHV-4) in cellular host range and integrin binding. Vet Res 2012 Aug 21;43(1):61.
- Azab W, Osterrieder N. Glycoproteins D of equine herpesvirus type 1 (EHV-1) and EHV-4 determine cellular tropism independently of integrins. J Virol 2012 Feb;86(4):2031-44.
- Liu D, Zhao X, Wang X. The Genomic Characterization of Equid Alphaherpesviruses: Structure, Function, and Genetic Similarity. Vet Sci 2025 Mar 3;12(3).
- Takeishi M, Morikawa S, Kuwata R, Kawaminami M, Shimoda H, Isawa H, Maeda K, Yoshikawa Y. Characterization and arbovirus susceptibility of cultured CERNI cells derived from sika deer (Cervus nippon). In Vitro Cell Dev Biol Anim 2024 Sep;60(8):935-948.
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