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Animals : an open access journal from MDPI2022; 12(23); doi: 10.3390/ani12233261

Evaluation of a Comprehensive Profile of Salivary Analytes for the Diagnosis of the Equine Gastric Ulcer Syndrome.

Abstract: In this report, the measurement of salivary biomarkers as an aid for diagnosis of equine gastric ulcer syndrome (EGUS) was studied. A comprehensive panel of 23 salivary analytes was measured in the saliva of horses affected by EGUS and compared to healthy animals and horses with other diseases clinically similar to EGUS but with a negative diagnosis at gastroscopic examination. A total of 147 horses were included in the study and divided into heathy population (n = 12), the EGUS group (n = 110), and the group of horses with other diseases (n = 25). From the 23 analytes studied, 17 showed increased values in EGUS horses when compared to healthy ones, and uric acid, triglycerides, and calcium were significantly increased in horses with EGUS compared to the group of other diseases. The receiver operating characteristic curve analyses showed a modest but significant discriminatory power of those three analytes to identify EGUS from other diseases with similar symptoms. The discriminatory power enhanced when the results of the three analytes were combined. In conclusion, the results showed that selected salivary analytes could have potential use as biomarkers in horses with EGUS.
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Publication Date: 2022-11-23 PubMed ID: 36496782PubMed Central: PMC9740180DOI: 10.3390/ani12233261Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the potential use of salivary biomarkers for diagnosing equine gastric ulcer syndrome (EGUS), highlighting that three specific analytes – uric acid, triglycerides, and calcium – were significantly higher in affected horses compared to healthy ones or those with other similar diseases.

Objective and Methodology of the Study

  • The primary aim of this research was to evaluate a diverse panel of 23 salivary analytes and their potential usefulness in identifying horses affected by EGUS.
  • The researchers undertook this diagnosis by analysing the saliva of 147 horses separated into three groups: healthy horses, those affected by EGUS, and horses with other clinically similar diseases but without EGUS.
  • Diagnostic validity was assessed via a statistical technique named the receiver operating characteristic (ROC) curve analysis. This method helped the researchers determine the accuracy of the salivary analytes in distinguishing EGUS from other diseases.

Key Findings

  • The study revealed that 17 out of the 23 studied analytes presented higher values in EGUS-affected horses compared to healthy ones.
  • Of these, uric acid, triglycerides, and calcium were significantly elevated in horses with EGUS compared to those with other diseases.
  • The ROC curve analysis indicated a modest but significant power of these three analytes to differentiate EGUS from other similar conditions. The discriminatory power improved when the results of all three analytes were combined.

Conclusion and Implications

  • The study concludes that selected salivary analytes, specifically uric acid, triglycerides, and calcium, have potential use as biomarkers for EGUS in horses
  • The findings, if confirmed by further research, have significant implications for diagnosing EGUS. Currently, diagnosing EGUS is invasive and expensive, requiring gastroscopic examination. If a reliable, non-invasive test based on salivary analytes can be developed, it could offer a quicker and less traumatic method of diagnosis for the horses involved.

Cite This Article

APA
Muñoz-Prieto A, Cerón JJ, Rubio CP, Contreras-Aguilar MD, Pardo-Marín L, Ayala-de la Peña I, Martín-Cuervo M, Holm Henriksen IM, Arense-Gonzalo JJ, Tecles F, Hansen S. (2022). Evaluation of a Comprehensive Profile of Salivary Analytes for the Diagnosis of the Equine Gastric Ulcer Syndrome. Animals (Basel), 12(23). https://doi.org/10.3390/ani12233261

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 23

Researcher Affiliations

Muñoz-Prieto, Alberto
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain.
  • Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia.
Cerón, José J
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain.
Rubio, Camila P
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain.
Contreras-Aguilar, María Dolores
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain.
Pardo-Marín, Luis
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain.
Ayala-de la Peña, Ignacio
  • Animal Medicine and Surgery Department, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain.
Martín-Cuervo, María
  • Animal Medicine, Faculty of Veterinary Medicine of Cáceres, University of Extremadura, Av. de la Universidad S-N, 10005 Cáceres, Spain.
Holm Henriksen, Ida-Marie
  • Department of Veterinary Clinical Sciences, Veterinary School of Medicine, Sektion Medicine and Surgery, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Høje-Taastrup, Denmark.
Arense-Gonzalo, Julián J
  • Institute for Biomedical Research of Murcia, IMIB-Arrixaca, El Palmar, 30120 Murcia, Spain.
  • Division of Preventive Medicine and Public Health Sciences, School of Medicine, University of Murcia, Espinardo, 30100 Murcia, Spain.
Tecles, Fernando
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain.
Hansen, Sanni
  • Department of Veterinary Clinical Sciences, Veterinary School of Medicine, Sektion Medicine and Surgery, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Høje-Taastrup, Denmark.

Grant Funding

  • 19894/GERM/15 / Fundación Seneca
  • FJC2019-042475-I / Ministry of Economy, Industry and Competitiveness

Conflict of Interest Statement

The authors declare no conflict of interest.

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