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Evaluation of prolactin receptor (PRLR) as candidate gene for male fertility in Hanoverian warmblood horses.
Abstract: Stallion fertility has increasing importance as the artificial insemination is employed in horses more intensely. Molecular genetic markers may be useful tools to evaluate the stallion fertility before breeding. The prolactin receptor gene (PRLR) was chosen as a candidate for stallion fertility because of its influence on testicular and accessory sex gland function. Screening the equine PRLR gene for polymorphisms in Hanoverian stallions revealed two single nucleotide polymorphisms (SNPs). Association and haplotype analyses were performed in 162 Hanoverian warmblood stallions for these intragenic SNPs using the least square means (LSM) of the pregnancy rate per oestrus for stallions and the paternal component and embryonic component of the breeding values (BV) of the pregnancy rate per oestrus. The two SNPs (BIEC2-589441, BIEC2-560860) showed significant associations using single marker and haplotype analysis with the embryonic and paternal component of BV and one SNP (BIEC2-560860) was also significantly associated with the LSM of the pregnancy rate per oestrus. This is the first report on an association of PRLR-associated genetic markers with fertility traits in stallions.
© 2009 Blackwell Verlag GmbH.
Publication Date: 2009-10-23 PubMed ID: 19845882DOI: 10.1111/j.1439-0531.2009.01533.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research studied the role of the prolactin receptor gene (PRLR) in male fertility of Hanoverian warmblood horses, finding specific gene variations linked to fertility traits.
Research Objective and Methods
The research aimed to gain insights into the role of prolactin receptor gene (PRLR) in stallion fertility, as its impact on testicular function and sex gland operation suggested it could influence fertility. Artificial insemination is increasingly common in horse breeding, making an understanding of stallion fertility critical.
- The researchers screened the PRLR gene for variations, known as polymorphisms, in Hanoverian stallions, discovering two single nucleotide polymorphisms (SNPs), or minute alterations in the DNA sequence.
- They analyzed these SNPs in 162 Hanoverian stallions regarding their association and sequence.
- They applied a statistical method known as least square means (LSM) to assess the pregnancy rate per oestrus, examining both the paternal and embryonic components.
Findings
The researchers found substantial associations between the examined SNPs and the embryonic and paternal components of fertility.
- Both identified SNPs showed significant relationships with the components of fertility using single marker and sequence analysis.
- One of the SNPs also had a prominent association with the LSM of the pregnancy rate per oestrus.
Significance
The research is the first to document an association between PRLR-linked genetic markers and fertility traits in stallions. It suggests certain genetic markers can help predict stallion fertility, providing valuable tools for breeders. More broadly, it contributes valuable knowledge to the field of equine genetics, potentially leading to improved breeding strategies and outcomes.
Cite This Article
APA
Giesecke K, Hamann H, Sieme H, Distl O.
(2009).
Evaluation of prolactin receptor (PRLR) as candidate gene for male fertility in Hanoverian warmblood horses.
Reprod Domest Anim, 45(5), e124-e130.
https://doi.org/10.1111/j.1439-0531.2009.01533.x Publication
Researcher Affiliations
- Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Hannover, Germany.
MeSH Terms
- Animals
- Gene Expression Regulation / physiology
- Genetic Markers / physiology
- Genotype
- Horses / genetics
- Horses / physiology
- Infertility, Male / genetics
- Male
- Polymorphism, Single Nucleotide
- Receptors, Prolactin / genetics
Citations
This article has been cited 8 times.- Khan MZ, Chen W, Naz S, Liu X, Liang H, Chen Y, Kou X, Liu Y, Ashraf I, Han Y, Peng Y, Wang C, Zahoor M. Determinant genetic markers of semen quality in livestock. Front Endocrinol (Lausanne) 2024;15:1456305.
- Gonzalez-Castro RA, Carnevale EM. Phospholipase C Zeta 1 (PLCZ1): The Function and Potential for Fertility Assessment and In Vitro Embryo Production in Cattle and Horses. Vet Sci 2023 Dec 11;10(12).
- Gmel AI, Burger D, Neuditschko M. A Novel QTL and a Candidate Gene Are Associated with the Progressive Motility of Franches-Montagnes Stallion Spermatozoa after Thaw. Genes (Basel) 2021 Sep 25;12(10).
- Nikitkina E, Krutikova A, Musidray A, Plemyashov K. Search for Associations of FSHR, INHA, INHAB, PRL, TNP2 and SPEF2 Genes Polymorphisms with Semen Quality in Russian Holstein Bulls (Pilot Study). Animals (Basel) 2021 Oct 2;11(10).
- Laseca N, Anaya G, Peña Z, Pirosanto Y, Molina A, Demyda Peyrás S. Impaired Reproductive Function in Equines: From Genetics to Genomics. Animals (Basel) 2021 Feb 3;11(2).
- Neis A, Kremer FS, Pinto LS, Leon PMM. In silico prediction of prolactin molecules as a tool for equine genomics reproduction. Mol Divers 2019 Nov;23(4):1019-1028.
- Schrimpf R, Gottschalk M, Metzger J, Martinsson G, Sieme H, Distl O. Screening of whole genome sequences identified high-impact variants for stallion fertility. BMC Genomics 2016 Apr 14;17:288.
- Schrimpf R, Dierks C, Martinsson G, Sieme H, Distl O. Genome-wide association study identifies phospholipase C zeta 1 (PLCz1) as a stallion fertility locus in Hanoverian warmblood horses. PLoS One 2014;9(10):e109675.
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