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Research in veterinary science2006; 81(2); 237-245; doi: 10.1016/j.rvsc.2005.12.008

Evaluation of single and double centrifugation tube methods for concentrating equine platelets.

Abstract: The aim of this study was to evaluate single and double centrifugation tube methods for concentrating equine platelets. Whole blood samples were collected from clinically normal horses and processed by use of single and double centrifugation tube methods to obtain four platelet concentrates (PCs): PC-A, PC-B, PC-C, and PC-D, which were analyzed using a flow cytometry hematology system for hemogram and additional platelet parameters (mean platelet volume, platelet distribution width, mean platelet component concentration, mean platelet component distribution width). Concentrations of transforming growth factor beta 1 (TGF-beta(1)) were determined in all the samples. Platelet concentrations for PC-A, PC-B, PC-C, and PC-D were 45%, 44%, 71%, and 21% higher, respectively, compared to the same values for citrated whole blood samples. TGF-beta(1) concentrations for PC-A, PC-B, PC-C, and PC-D were 38%, 44%, 44%, and 37% higher, respectively, compared to citrated whole blood sample values. In conclusion, the single and double centrifugation tube methods are reliable methods for concentrating equine platelets and for obtaining potentially therapeutic TGF-beta(1) levels.
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Publication Date: 2006-09-14 PubMed ID: 16969921DOI: 10.1016/j.rvsc.2005.12.008Google Scholar: Lookup
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  • Evaluation Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study evaluates two methods (single and double centrifugation tube methods) for concentrating horse platelets and their efficiency in obtaining potentially therapeutic TGF-beta(1) levels. The study shows that both methods are reliable and result in higher concentrations of platelets and TGF-beta(1) compared to citrated whole blood samples.

Research Methods

  • The study used whole blood samples from healthy horses which were processed using single and double centrifugation tube methods.
  • Four different platelet concentrates (PC-A, PC-B, PC-C, and PC-D) were obtained and analyzed using a flow cytometry hematology system.
  • Parameters such as hemogram, mean platelet volume, platelet distribution width, mean platelet component concentration, and mean platelet component distribution width were examined.
  • The concentrations of a protein called transforming growth factor beta 1 (TGF-beta(1)) were also determined across all samples.

Findings

  • The platelet concentrations for the four PCs (PC-A, PC-B, PC-C, and PC-D) were 45%, 44%, 71%, and 21% higher, respectively, compared to the values for citrated whole blood samples.
  • Similarly, TGF-beta(1) concentrations for PC-A, PC-B, PC-C, and PC-D were 38%, 44%, 44%, and 37% higher, respectively, compared to citrated whole blood sample values.

Conclusion

  • Both the single and double centrifugation tube methods were found to be reliable methods for concentrating equine platelets.
  • Additionally, these methods were efficient in obtaining higher levels of potentially therapeutic TGF-beta(1).
  • Overall, these methods could be effectively employed for preparing platelet concentrates for use in equine therapeutic treatments.

Cite This Article

APA
Argüelles D, Carmona JU, Pastor J, Iborra A, Viñals L, Martínez P, Bach E, Prades M. (2006). Evaluation of single and double centrifugation tube methods for concentrating equine platelets. Res Vet Sci, 81(2), 237-245. https://doi.org/10.1016/j.rvsc.2005.12.008

Publication

Research in veterinary science
ISSN: 0034-5288
NlmUniqueID: 0401300
Country: England
Language: English
Volume: 81
Issue: 2
Pages: 237-245

Researcher Affiliations

Argüelles, D
  • Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Spain.
Carmona, J U
    Pastor, J
      Iborra, A
        Viñals, L
          Martínez, P
            Bach, E
              Prades, M

                MeSH Terms

                • Animals
                • Blood Platelets / cytology
                • Centrifugation / methods
                • Centrifugation / veterinary
                • Enzyme-Linked Immunosorbent Assay / veterinary
                • Female
                • Flow Cytometry / veterinary
                • Horses / blood
                • Male
                • Platelet Count / veterinary
                • Statistics, Nonparametric
                • Transforming Growth Factor beta / analysis

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