Evaluation of the Honey-Card Technique for Detection of Transmission of Arboviruses in Florida and Comparison With Sentinel Chicken Seroconversion.
Abstract: Zoonotic mosquito-borne viruses, such as the West Nile virus (WNV) and eastern equine encephalitis virus (EEEV), are major public health threats in the United States. Early detection of virus transmission and targeted vector management are critical to protect humans against these pathogens. Sentinel chickens and pool screening of mosquitoes, the most widely used methods of arbovirus early detection, have technical time-lags that compromise their early-detection value. The exploitation of sugar-feeding by trapped mosquitoes for arbovirus surveillance may represent a viable alternative to other methods. Here we compared effectiveness of sugar-impregnated nucleic-acid preserving substrates (SIPS) and sentinel chicken program for detecting WNV, EEEV, and St. Louis encephalitis virus in gravid traps, CO-baited light traps, and resting traps at 10 locations in two Florida counties. In St. Johns County, comparable numbers of EEEV detections were made by SIPS traps (18) and sentinel chickens (22), but fewer WNV detections were made using SIPS (1) than sentinel chickens (13). In Volusia County, seven arbovirus detections were made via the sentinel chicken program (one EEEV and six WNV), whereas only one arbovirus detection (WNV) was made using SIPS. CO-baited light traps captured >90% of total mosquitoes, yet yielded <30% of arbovirus detections. Resting traps and gravid traps captured a fraction of total mosquitoes, yet yielded roughly equivalent numbers of arbovirus detections, as did light traps. Challenges to successful deployment of SIPS include optimization of traps for collecting all vector species, increasing sugar-feeding rates of trapped vectors, and developing tractable methods for arbovirus detection.
© The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.
Publication Date: 2016-06-21 PubMed ID: 27330092DOI: 10.1093/jme/tjw106Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Arboviruses
- Comparative Study
- Diagnosis
- Disease control
- Disease Diagnosis
- Disease Prevention
- Disease Surveillance
- Disease Transmission
- Encephalitis
- Epidemiology
- Equine Health
- Infectious Disease
- Mosquito-borne Diseases
- Pathogens
- Public Health
- Vector-borne disease
- Veterinary Medicine
- Veterinary Research
- Veterinary Science
- West Nile Virus
- Zoonotic Diseases
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The researchers analyzed the efficiency of a new method – using sugar-impregnated nucleic-acid preserving substrates (SIPS), also known as the Honey-card technique, for early detection of mosquito-borne viruses compared to the traditional method of using sentinel chickens. The study was conducted in two counties in Florida and evaluated on the basis of detection of West Nile virus, eastern equine encephalitis virus and St. Louis encephalitis virus.
Methodology
The study was conducted at 10 different locations in St. Johns and Volusia County, Florida. Detection rates of West Nile Virus (WNV), eastern equine encephalitis virus (EEEV), and St. Louis encephalitis virus were tested via two different methods: the traditional sentinel chicken program and the proposed SIPS technique. Three types of traps were used: CO2-baited light traps, resting traps, and gravid traps.
Results
- In St. Johns County, the SIPS method and the sentinel chicken program showed similar EEEV detection rates (18 for SIPS and 22 for sentinel chickens), but the SIPS technique lagged in WNV detection with only 1 detection as compared to 13 with sentinel chickens.
- In Volusia county, the sentinel chicken program detected seven arbovirus (one EEEV and six WNV) while SIPS detected only one (WNV).
- Most of the mosquitoes (>90%) were captured by the CO2-baited light traps, but this method resulted in less than 30% of arbovirus detections. On the other hand, resting traps and gravid traps captured much fewer mosquitoes but had equivalent arbovirus detection rates as the light traps.
Challenges and Solutions
The study identified multiple challenges for the successful implementation of the SIPS method:
- The traps need to be optimised for collecting all vector species.
- The sugar-feeding rates of the trapped vectors need to be increased.
- Effective methods for arbovirus detection need to developed and deployed.
Cite This Article
APA
Burkett-Cadena ND, Gibson J, Lauth M, Stenn T, Acevedo C, Xue RD, McNelly J, Northey E, Hassan HK, Fulcher A, Bingham AM, van Olphen J, van Olphen A, Unnasch TR.
(2016).
Evaluation of the Honey-Card Technique for Detection of Transmission of Arboviruses in Florida and Comparison With Sentinel Chicken Seroconversion.
J Med Entomol, 53(6), 1449-1457.
https://doi.org/10.1093/jme/tjw106 Publication
Researcher Affiliations
- University of Florida IFAS, Florida Medical Entomology Laboratory, 200 9th St. SE, Vero Beach, FL 32962 (nburkettcadena@ufl.edu; tanise@ufl.edu; c.acevedo@ufl.edu) nburkettcadena@ufl.edu.
- Anastasia Mosquito Control District, 500 Old Beach Road, St. Augustine, FL 32080 (gibsonamcd@bellsouth.net; xueamcd@gmail.com; aliamcd@bellsouth.net).
- Volusia County Mosquito Control, 801 South St, New Smyrna Beach, FL 32168 (mlauth@volusia.org; jmcnelly@volusia.org; enorthey@volusia.org).
- University of Florida IFAS, Florida Medical Entomology Laboratory, 200 9th St. SE, Vero Beach, FL 32962 (nburkettcadena@ufl.edu; tanise@ufl.edu; c.acevedo@ufl.edu).
- University of Florida IFAS, Florida Medical Entomology Laboratory, 200 9th St. SE, Vero Beach, FL 32962 (nburkettcadena@ufl.edu; tanise@ufl.edu; c.acevedo@ufl.edu).
- Anastasia Mosquito Control District, 500 Old Beach Road, St. Augustine, FL 32080 (gibsonamcd@bellsouth.net; xueamcd@gmail.com; aliamcd@bellsouth.net).
- Volusia County Mosquito Control, 801 South St, New Smyrna Beach, FL 32168 (mlauth@volusia.org; jmcnelly@volusia.org; enorthey@volusia.org).
- Volusia County Mosquito Control, 801 South St, New Smyrna Beach, FL 32168 (mlauth@volusia.org; jmcnelly@volusia.org; enorthey@volusia.org).
- Global Health Infectious Disease Program, University of South Florida, 3720 Spectrum Blvd., Tampa, FL 33612 (hhassan@health.usf.edu; Andrea.Bingham@flhealth.gov; jose19@mail.usf.edu; avanolp@clemson.edu; tunnasch@health.usf.edu).
- Anastasia Mosquito Control District, 500 Old Beach Road, St. Augustine, FL 32080 (gibsonamcd@bellsouth.net; xueamcd@gmail.com; aliamcd@bellsouth.net).
- Global Health Infectious Disease Program, University of South Florida, 3720 Spectrum Blvd., Tampa, FL 33612 (hhassan@health.usf.edu; Andrea.Bingham@flhealth.gov; jose19@mail.usf.edu; avanolp@clemson.edu; tunnasch@health.usf.edu).
- Present Address: Florida Department of Health, Division of Disease Control and Health Protection, Bureau of Epidemiology, 4052 Bald Cypress Way, Bin # A12, Tallahassee, Florida 32399-1710, and.
- Global Health Infectious Disease Program, University of South Florida, 3720 Spectrum Blvd., Tampa, FL 33612 (hhassan@health.usf.edu; Andrea.Bingham@flhealth.gov; jose19@mail.usf.edu; avanolp@clemson.edu; tunnasch@health.usf.edu).
- Global Health Infectious Disease Program, University of South Florida, 3720 Spectrum Blvd., Tampa, FL 33612 (hhassan@health.usf.edu; Andrea.Bingham@flhealth.gov; jose19@mail.usf.edu; avanolp@clemson.edu; tunnasch@health.usf.edu).
- Present Address: Clemson University, Clemson Veterinary Diagnostic Center, PO Box 102406, Columbia, South Carolina 29224-2406.
- Global Health Infectious Disease Program, University of South Florida, 3720 Spectrum Blvd., Tampa, FL 33612 (hhassan@health.usf.edu; Andrea.Bingham@flhealth.gov; jose19@mail.usf.edu; avanolp@clemson.edu; tunnasch@health.usf.edu).
MeSH Terms
- Animals
- Arboviruses / classification
- Arboviruses / isolation & purification
- Carbohydrates
- Chickens
- Culicidae / virology
- Encephalitis Virus, Eastern Equine / isolation & purification
- Encephalitis Virus, St. Louis / isolation & purification
- Female
- Florida
- Honey / statistics & numerical data
- Polymerase Chain Reaction
- Sentinel Surveillance / veterinary
- Seroconversion
- West Nile virus / isolation & purification
Citations
This article has been cited 11 times.- Manzi S, Nelli L, Fortuna C, Severini F, Toma L, Di Luca M, Michelutti A, Bertola M, Gradoni F, Toniolo F, Sgubin S, Lista F, Pazienza M, Montarsi F, Pombi M. A modified BG-Sentinel trap equipped with FTA card as a novel tool for mosquito-borne disease surveillance: a field test for flavivirus detection.. Sci Rep 2023 Aug 8;13(1):12840.
- Samsonova JV, Saushkin NY, Osipov AP. Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives.. Vet Res Commun 2022 Sep;46(3):655-698.
- Alomar AA, Eastmond BH, Alto BW. Juvenile hormone analog enhances Zika virus infection in Aedes aegypti.. Sci Rep 2021 Oct 26;11(1):21062.
- McMillan JR, Harden CA, Burtis JC, Breban MI, Shepard JJ, Petruff TA, Misencik MJ, Bransfield AB, Poggi JD, Harrington LC, Andreadis TG, Armstrong PM. The community-wide effectiveness of municipal larval control programs for West Nile virus risk reduction in Connecticut, USA.. Pest Manag Sci 2021 Nov;77(11):5186-5201.
- Guissou E, Waite JL, Jones M, Bell AS, Suh E, Yameogo KB, Djègbè N, Da DF, Hien DFDS, Yerbanga RS, Ouedraogo AG, Dabiré KR, Cohuet A, Thomas MB, Lefèvre T. A non-destructive sugar-feeding assay for parasite detection and estimating the extrinsic incubation period of Plasmodium falciparum in individual mosquito vectors.. Sci Rep 2021 Apr 29;11(1):9344.
- Honório NA, Câmara DCP, Wiggins K, Eastmond B, Alto BW. High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus.. Viruses 2020 Nov 23;12(11).
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- Glushakova LG, Alto BW, Kim MS, Hutter D, Bradley A, Bradley KM, Burkett-Cadena ND, Benner SA. Multiplexed kit based on Luminex technology and achievements in synthetic biology discriminates Zika, chikungunya, and dengue viruses in mosquitoes.. BMC Infect Dis 2019 May 14;19(1):418.
- Ramírez AL, Hall-Mendelin S, Doggett SL, Hewitson GR, McMahon JL, Ritchie SA, van den Hurk AF. Mosquito excreta: A sample type with many potential applications for the investigation of Ross River virus and West Nile virus ecology.. PLoS Negl Trop Dis 2018 Aug;12(8):e0006771.
- Glushakova LG, Alto BW, Kim MS, Wiggins K, Eastmond B, Moussatche P, Burkett-Cadena ND, Benner SA. Optimization of cationic (Q)-paper for detection of arboviruses in infected mosquitoes.. J Virol Methods 2018 Nov;261:71-79.
- Burkhalter KL, Wiggins K, Burkett-Cadena N, Alto BW. Laboratory Evaluation of Commercially Available Platforms to Detect West Nile and Zika Viruses From Honey Cards.. J Med Entomol 2018 May 4;55(3):717-722.
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