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Home / Equine Research Bank / J Equine Vet Sci / Evaluation of Three Methods of Platelet-Rich Plasma for Trea...
Journal of equine veterinary science2017; 72; 1-7; doi: 10.1016/j.jevs.2017.10.009

Evaluation of Three Methods of Platelet-Rich Plasma for Treatment of Equine Distal Limb Skin Wounds.

Abstract: Wounds localized on the distal limbs in horses are difficulty healing. No studies have been conducted to evaluate homologous platelet-rich plasma (PRP) in horses. The aim of this study was to evaluate the effect upon applying autologous PRP, homologous PRP, and autologous PRP gel on the wound healing process of wounds on the distal limb of horses. To study a possible correlation between the platelet count and wound healing time, four skin wounds of 4 cm2 were surgically created on the dorsolateral aspect of the third metacarpus in eight healthy adult horses. Each skin wound was randomly treated according to the established treatment groups (G): GI, treated with autologous PRP injection on the wound edges; GII treated topically with autologous PRP gel; and GIII homologous PRP injection on the wound edges and control group treated with saline solution injected on the wound edges. The animals were monitored every fifteen days throughout the wound healing process. The wound size was determined using a tape measure. Skin biopsies were obtained on the 15th and 30th day after the first PRP treatment. Autologous PRP gel treatment reduced the healing time in 15 days compared to the wounds of the control group. Histologically, GII presented a higher frequency of mild inflammation and mild to moderate neovascularization of biopsies 1 and 2. Platelet-rich plasma in gel form showed the best result on wound healing of the distal limb of horses in both clinical and histopathological evaluations. The homologous PRP is recommended for the recovery of animals with compromised hemodynamic conditions.
Copyright © 2017 Elsevier Inc. All rights reserved.
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Publication Date: 2017-10-14 PubMed ID: 30929771DOI: 10.1016/j.jevs.2017.10.009Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on assessing the efficacy of three methods of Platelet-Rich Plasma (PRP) – autologous PRP, autologous PRP gel and homologous PRP – in the treatment of equine distal limb skin wounds and how healing time correlates with platelet count. It was found that the autologous PRP gel treatment was most effective, reducing wound healing time by 15 days compared with the control group.

Research Methodology

  • Eight healthy adult horses were used for the study.
  • Four skin wounds of 4 cm each were surgically created on the dorsolateral aspect of the third metacarpus in each horse.
  • Each wound was randomly assigned one of the three different PRP treatments or a saline solution (acting as a control).
  • The horses were monitored every two weeks throughout the wound healing process, with wound size being measured using a tape.
  • Skin biopsies were taken on the 15th and 30th day after initial PRP treatment to investigate the microscopic effects of the treatments.

Findings

  • Autologous PRP gel treatment was found to reduce wound healing time by 15 days compared to wounds treated with saline solution.
  • Under histological examination, wounds treated with autologous PRP gel showed mild inflammation and mild to moderate new blood vessel formation. This indicates a positive response to wound healing.
  • The study findings suggest that the gel form of PRP effectively accelerates wound healing, superior to other PRP treatments and saline solution.

Recommendations

  • Homologous PRP, although not demonstrating the same efficacy as autologous PRP gel, is recommended for the recovery of horses with compromised hemodynamic conditions. This is because it is derived from a donor, allowing for treatment without potentially compromising the animal’s own blood supply.
  • The study provides valuable insight into equine wound care and suggests the potential expansion of PRP treatments in equine medicine.

Cite This Article

APA
Pereira RCDF, De La Côrte FD, Brass KE, da Silva Azevedo M, Gallio M, Cantarelli C, Dau SL, Cezar AS, Inkelmann MA. (2017). Evaluation of Three Methods of Platelet-Rich Plasma for Treatment of Equine Distal Limb Skin Wounds. J Equine Vet Sci, 72, 1-7. https://doi.org/10.1016/j.jevs.2017.10.009

Publication

Journal of equine veterinary science
ISSN: 0737-0806
NlmUniqueID: 8216840
Country: United States
Language: English
Volume: 72
Pages: 1-7

Researcher Affiliations

Pereira, Roberta Carneiro da Fontoura
  • Department of Agrarian Studies, Regional State University of Northwestern Rio Grande do Sul, Rio Grande do Sul, Ijuí, Brazil. Electronic address: betacfp@hotmail.com.
De La Côrte, Flávio Desessards
  • Department of Large Animal Clinics, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Brass, Karin Erica
  • Department of Large Animal Clinics, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
da Silva Azevedo, Marcos
  • Department of Veterinary Medicine, Federal University of Pampa, Uruguaiana, Rio Grande do Sul, Brazil.
Gallio, Miguel
  • Department of Large Animal Clinics, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Cantarelli, Camila
  • Department of Large Animal Clinics, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Dau, Stefano Leite
  • Department of Large Animal Clinics, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Cezar, Alfredo Skrebsky
  • Department of Agrarian Studies, Regional State University of Northwestern Rio Grande do Sul, Rio Grande do Sul, Ijuí, Brazil.
Inkelmann, Maria Andréia
  • Department of Agrarian Studies, Regional State University of Northwestern Rio Grande do Sul, Rio Grande do Sul, Ijuí, Brazil.

MeSH Terms

  • Animals
  • Extremities
  • Horses
  • Platelet-Rich Plasma
  • Skin / injuries
  • Soft Tissue Injuries / veterinary
  • Wound Healing

Citations

This article has been cited 23 times.
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