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Journal of equine veterinary science2017; 72; 1-7; doi: 10.1016/j.jevs.2017.10.009

Evaluation of Three Methods of Platelet-Rich Plasma for Treatment of Equine Distal Limb Skin Wounds.

Abstract: Wounds localized on the distal limbs in horses are difficulty healing. No studies have been conducted to evaluate homologous platelet-rich plasma (PRP) in horses. The aim of this study was to evaluate the effect upon applying autologous PRP, homologous PRP, and autologous PRP gel on the wound healing process of wounds on the distal limb of horses. To study a possible correlation between the platelet count and wound healing time, four skin wounds of 4 cm2 were surgically created on the dorsolateral aspect of the third metacarpus in eight healthy adult horses. Each skin wound was randomly treated according to the established treatment groups (G): GI, treated with autologous PRP injection on the wound edges; GII treated topically with autologous PRP gel; and GIII homologous PRP injection on the wound edges and control group treated with saline solution injected on the wound edges. The animals were monitored every fifteen days throughout the wound healing process. The wound size was determined using a tape measure. Skin biopsies were obtained on the 15th and 30th day after the first PRP treatment. Autologous PRP gel treatment reduced the healing time in 15 days compared to the wounds of the control group. Histologically, GII presented a higher frequency of mild inflammation and mild to moderate neovascularization of biopsies 1 and 2. Platelet-rich plasma in gel form showed the best result on wound healing of the distal limb of horses in both clinical and histopathological evaluations. The homologous PRP is recommended for the recovery of animals with compromised hemodynamic conditions.
Copyright © 2017 Elsevier Inc. All rights reserved.
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Publication Date: 2017-10-14 PubMed ID: 30929771DOI: 10.1016/j.jevs.2017.10.009Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on assessing the efficacy of three methods of Platelet-Rich Plasma (PRP) – autologous PRP, autologous PRP gel and homologous PRP – in the treatment of equine distal limb skin wounds and how healing time correlates with platelet count. It was found that the autologous PRP gel treatment was most effective, reducing wound healing time by 15 days compared with the control group.

Research Methodology

  • Eight healthy adult horses were used for the study.
  • Four skin wounds of 4 cm each were surgically created on the dorsolateral aspect of the third metacarpus in each horse.
  • Each wound was randomly assigned one of the three different PRP treatments or a saline solution (acting as a control).
  • The horses were monitored every two weeks throughout the wound healing process, with wound size being measured using a tape.
  • Skin biopsies were taken on the 15th and 30th day after initial PRP treatment to investigate the microscopic effects of the treatments.

Findings

  • Autologous PRP gel treatment was found to reduce wound healing time by 15 days compared to wounds treated with saline solution.
  • Under histological examination, wounds treated with autologous PRP gel showed mild inflammation and mild to moderate new blood vessel formation. This indicates a positive response to wound healing.
  • The study findings suggest that the gel form of PRP effectively accelerates wound healing, superior to other PRP treatments and saline solution.

Recommendations

  • Homologous PRP, although not demonstrating the same efficacy as autologous PRP gel, is recommended for the recovery of horses with compromised hemodynamic conditions. This is because it is derived from a donor, allowing for treatment without potentially compromising the animal’s own blood supply.
  • The study provides valuable insight into equine wound care and suggests the potential expansion of PRP treatments in equine medicine.

Cite This Article

APA
Pereira RCDF, De La Côrte FD, Brass KE, da Silva Azevedo M, Gallio M, Cantarelli C, Dau SL, Cezar AS, Inkelmann MA. (2017). Evaluation of Three Methods of Platelet-Rich Plasma for Treatment of Equine Distal Limb Skin Wounds. J Equine Vet Sci, 72, 1-7. https://doi.org/10.1016/j.jevs.2017.10.009

Publication

Journal of equine veterinary science
ISSN: 0737-0806
NlmUniqueID: 8216840
Country: United States
Language: English
Volume: 72
Pages: 1-7

Researcher Affiliations

Pereira, Roberta Carneiro da Fontoura
  • Department of Agrarian Studies, Regional State University of Northwestern Rio Grande do Sul, Rio Grande do Sul, Ijuí, Brazil. Electronic address: betacfp@hotmail.com.
De La Côrte, Flávio Desessards
  • Department of Large Animal Clinics, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Brass, Karin Erica
  • Department of Large Animal Clinics, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
da Silva Azevedo, Marcos
  • Department of Veterinary Medicine, Federal University of Pampa, Uruguaiana, Rio Grande do Sul, Brazil.
Gallio, Miguel
  • Department of Large Animal Clinics, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Cantarelli, Camila
  • Department of Large Animal Clinics, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Dau, Stefano Leite
  • Department of Large Animal Clinics, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Cezar, Alfredo Skrebsky
  • Department of Agrarian Studies, Regional State University of Northwestern Rio Grande do Sul, Rio Grande do Sul, Ijuí, Brazil.
Inkelmann, Maria Andréia
  • Department of Agrarian Studies, Regional State University of Northwestern Rio Grande do Sul, Rio Grande do Sul, Ijuí, Brazil.

MeSH Terms

  • Animals
  • Extremities
  • Horses
  • Platelet-Rich Plasma
  • Skin / injuries
  • Soft Tissue Injuries / veterinary
  • Wound Healing

Citations

This article has been cited 23 times.
  1. Hussein KH, Soliman M, Abd-Elkareem M, Sadek AA. Comparative evaluation of fresh and lyophilized Nile tilapia fish skin for enhancing wound healing in a donkey model. Vet Res Commun 2025 Jul 23;49(5):262.
    doi: 10.1007/s11259-025-10821-wpubmed: 40699393google scholar: lookup
  2. Albahrawy M, El-Henawey MI, Elmezayyen AS, Mosbah E, Karrouf G, Zaghloul A, Abass M. Ameliorative role of silver nanoparticles incorporated with chitosan solution and leukocyte platelet-rich fibrin scaffold during colon anastomosis in rabbits. J Mater Sci Mater Med 2025 Jul 8;36(1):58.
    doi: 10.1007/s10856-025-06908-0pubmed: 40627202google scholar: lookup
  3. Gottardo A, Tulone G, Pavan N, Fulfaro F, Gristina V, Bazan Russo TD, Prestifilippo O, Claps F, Incorvaia L, Galvano A, Russo A, Simonato A. Applications of Platelet Concentrates (PCs) in Regenerative Onco-Urology: A Systematic Review of Literature. Int J Mol Sci 2024 Oct 4;25(19).
    doi: 10.3390/ijms251910683pubmed: 39409012google scholar: lookup
  4. Wang X, Wang Y, Li J, Han Y, Wei M, Xu A, Xin L. Effects of intrauterine infusion of autologous platelet-rich plasma gel on endometrial thickness and outcomes of frozen-thawed embryo transfer in women with thin endometrium. J Assist Reprod Genet 2024 Oct;41(10):2635-2647.
    doi: 10.1007/s10815-024-03254-ypubmed: 39269566google scholar: lookup
  5. Bernardini C, Romagnoli N, Casalini I, Turba ME, Spadari A, Forni M, Gentilini F. Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate. Int J Vet Sci Med 2024;12(1):71-80.
    doi: 10.1080/23144599.2024.2380586pubmed: 39119550google scholar: lookup
  6. Ribeiro G, Carvalho L, Borges J, Prazeres J. The Best Protocol to Treat Equine Skin Wounds by Second Intention Healing: A Scoping Review of the Literature. Animals (Basel) 2024 May 18;14(10).
    doi: 10.3390/ani14101500pubmed: 38791717google scholar: lookup
  7. Reis IL, Lopes B, Sousa P, Sousa AC, Caseiro AR, Mendonça CM, Santos JM, Atayde LM, Alvites RD, Maurício AC. Equine Musculoskeletal Pathologies: Clinical Approaches and Therapeutical Perspectives-A Review. Vet Sci 2024 Apr 26;11(5).
    doi: 10.3390/vetsci11050190pubmed: 38787162google scholar: lookup
  8. Albahrawy M, Abouelnasr K, Mosbah E, Zaghloul A, Abass M. Biostimulation effect of platelet-rich fibrin augmented with decellularized bovine pericardium on full-thickness cutaneous wound healing in Donkeys (Equus asinus). BMC Vet Res 2023 Sep 20;19(1):166.
    doi: 10.1186/s12917-023-03733-xpubmed: 37730587google scholar: lookup
  9. Iacopetti I, Perazzi A, Patruno M, Contiero B, Carolo A, Martinello T, Melotti L. Assessment of the quality of the healing process in experimentally induced skin lesions treated with autologous platelet concentrate associated or unassociated with allogeneic mesenchymal stem cells: preliminary results in a large animal model. Front Vet Sci 2023;10:1219833.
    doi: 10.3389/fvets.2023.1219833pubmed: 37559892google scholar: lookup
  10. Bakadia BM, Qaed Ahmed AA, Lamboni L, Shi Z, Mutu Mukole B, Zheng R, Pierre Mbang M, Zhang B, Gauthier M, Yang G. Engineering homologous platelet-rich plasma, platelet-rich plasma-derived exosomes, and mesenchymal stem cell-derived exosomes-based dual-crosslinked hydrogels as bioactive diabetic wound dressings. Bioact Mater 2023 Oct;28:74-94.
    doi: 10.1016/j.bioactmat.2023.05.002pubmed: 37234363google scholar: lookup
  11. Afonso AC, Sousa M, Pinto AR, Cotovio M, Simões M, Saavedra MJ. Biofilm Production by Critical Antibiotic-Resistant Pathogens from an Equine Wound. Animals (Basel) 2023 Apr 13;13(8).
    doi: 10.3390/ani13081342pubmed: 37106905google scholar: lookup
  12. Troha K, Vozel D, Arko M, Bedina Zavec A, Dolinar D, Hočevar M, Jan Z, Kisovec M, Kocjančič B, Pađen L, Pajnič M, Penič S, Romolo A, Repar N, Spasovski V, Steiner N, Šuštar V, Iglič A, Drobne D, Kogej K, Battelino S, Kralj-Iglič V. Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review. Int J Mol Sci 2023 Feb 8;24(4).
    doi: 10.3390/ijms24043420pubmed: 36834843google scholar: lookup
  13. Albahrawy M, Abouelnasr K, Mosbah E, Zaghloul A, Abass M. Acellular bovine pericardium as a biological dressing for treatment of cutaneous wounds of the distal limb in donkeys (Equus Asinus). Vet Res Commun 2023 Jun;47(2):587-597.
    doi: 10.1007/s11259-022-10014-9pubmed: 36323838google scholar: lookup
  14. Helal IE, Al-Abbadi HA, El-Daharawy MH, Ahmed MF. Enhancement of chronic wound healing with maltodextrin/ascorbic acid gel: a clinical evaluation of distal limb wounds in horses. J Anim Sci Technol 2022 Sep;64(5):997-1007.
    doi: 10.5187/jast.2022.e52pubmed: 36287738google scholar: lookup
  15. Seidel SRT, de Souza AF, Fülber J, Bogossian PM, Rodrigues NNP, Baccarin RYA. Evaluation of platelet-rich plasma applied in the coronary band of healthy equine hooves. Can Vet J 2021 Jul;62(7):729-735.
    pubmed: 34219782
  16. Segabinazzi LGTM, Podico G, Rosser MF, Nanjappa SG, Alvarenga MA, Canisso IF. Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma. Animals (Basel) 2021 May 21;11(6).
    doi: 10.3390/ani11061478pubmed: 34063777google scholar: lookup
  17. Segabinazzi LGTM, Canisso IF, Podico G, Cunha LL, Novello G, Rosser MF, Loux SC, Lima FS, Alvarenga MA. Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares. Antibiotics (Basel) 2021 Apr 23;10(5).
    doi: 10.3390/antibiotics10050490pubmed: 33922743google scholar: lookup
  18. Camargo Garbin L, Lopez C, Carmona JU. A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade. Front Vet Sci 2021;8:641818.
    doi: 10.3389/fvets.2021.641818pubmed: 33869321google scholar: lookup
  19. Mohammadi Najafabadi M, Soleimani M, Ahmadvand M, Soufi Zomorrod M, Mousavi SA. Treatment protocols for BK virus associated hemorrhagic cystitis after hematopoietic stem cell transplantation. Am J Blood Res 2020;10(5):217-230.
    pubmed: 33224566
  20. Iacopetti I, Patruno M, Melotti L, Martinello T, Bedin S, Badon T, Righetto EM, Perazzi A. Autologous Platelet-Rich Plasma Enhances the Healing of Large Cutaneous Wounds in Dogs. Front Vet Sci 2020;7:575449.
    doi: 10.3389/fvets.2020.575449pubmed: 33195571google scholar: lookup
  21. Perego R, Spada E, Baggiani L, Martino PA, Proverbio D. Efficacy of a Semi Automated Commercial Closed System for Autologous Leukocyte- and Platelet-Rich Plasma (l-prp) Production in Dogs: A Preliminary Study. Animals (Basel) 2020 Aug 4;10(8).
    doi: 10.3390/ani10081342pubmed: 32759643google scholar: lookup
  22. Canisso IF, Segabinazzi LGTM, Fedorka CE. Persistent Breeding-Induced Endometritis in Mares - a Multifaceted Challenge: From Clinical Aspects to Immunopathogenesis and Pathobiology. Int J Mol Sci 2020 Feb 20;21(4).
    doi: 10.3390/ijms21041432pubmed: 32093296google scholar: lookup
  23. Masieri L, Sessa F, Mari A, Campi R, Cito G, Verrienti P, Nozzoli C, Saccardi R, Sforza S, Di Maida F, Grosso AA, Carini M, Minervini A. Intravesical application of platelet-rich plasma in patients with persistent haemorrhagic cystitis after hematopoietic stem cell transplantation: a single-centre preliminary experience. Int Urol Nephrol 2019 Oct;51(10):1715-1720.
    doi: 10.1007/s11255-019-02223-0pubmed: 31321678google scholar: lookup
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