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Animals : an open access journal from MDPI2024; 14(20); 2937; doi: 10.3390/ani14202937

Evaluation of Two Commercial ELISA Kits for Measuring Equine Serum Gastrin Compared to Radioimmunoassay.

Abstract: Gastrin is an important hormone involved in gastric acid secretion. Despite its importance, validated methods other than radioimmunoassay (RIA) to assess serum gastrin concentrations in horses are lacking. This study aims to determine the agreement between ELISA and RIA in quantifying equine serum gastrin concentrations. Serum gastrin concentrations were quantified using two ELISA kits and RIA. Samples (196) from 14 horses at different time points were analyzed using one ELISA kit and RIA, selected samples (7) were analyzed using a second ELISA kit, and the correlation between methods was calculated. The level of agreement was analyzed by Bland-Altman analysis and differences between ELISA and RIA were plotted against averages for each sample. The Pearson correlation between gastrin concentrations measured by ELISA and the RIA was 0.27 and -0.32 for ELISA kit 1 and kit 2, respectively. Mean bias (ELISA-RIA) was 198.40 pg/mL (95% CI: -142.95-539.76) and -17.90 pg/mL (95% CI: -89.98-54.19) for ELISA kit 1 and kit 2, respectively. Measurements of horse gastrin by both ELISA methods were highly variable, with an unacceptable correlation to the reference method, RIA. Using non-validated ELISA methods to quantify horse gastrin cannot be recommended.
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Publication Date: 2024-10-11 PubMed ID: 39457867PubMed Central: PMC11503838DOI: 10.3390/ani14202937Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study involves a comparison between two commercial Enzyme-Linked Immunosorbent Assay (ELISA) kits and Radioimmunoassay (RIA) for measuring equine serum gastrin. The outcomes suggest that the ELISA methods are highly variable and do not align well with the reference method, RIA, therefore they can’t be recommended for quantifying horse gastrin.

Objective of the Study

  • The primary goal of the research was to evaluate the agreement between ELISA kits and RIA in measuring equine serum gastrin concentrations, which plays a crucial role in gastric acid secretion in horses.

Methodology

  • The researchers measured serum gastrin concentrations in 14 horses at different time points using two different ELISA kits and RIA.
  • The selected samples from these horses (196 samples in total) were analyzed using one ELISA kit and RIA, while a second ELISA kit was used for a selected sample set (7 samples).
  • The resulting measures were correlated to gauge the level of agreement between the methods.

Results and Discussion

  • The correlation between the gastrin measurements obtained through the ELISA methods and the RIA was found to be largely incoherent.
  • The Pearson correlation was calculated at 0.27 and -0.32 for the first and second ELISA kit, respectively. These correlations indicate a low degree of agreement.
  • The mean bias (the average error between the ELISA and RIA results) was calculated as 198.40 pg/mL for the first ELISA kit and -17.90 pg/mL for the second ELISA kit.

Conclusion

  • The study concluded that measurements of horse gastrin by both ELISA methods were highly variable, indicating a lack of consistency.
  • As such, these types of non-validated ELISA methods are not recommended for the quantification of horse gastrin due to their weak correlation with the reference method, which is RIA.

Cite This Article

APA
Vokes JR, Gedye KR, Lovett AL, de Kantzow MC, Shan R, Steel CM, Sykes BW. (2024). Evaluation of Two Commercial ELISA Kits for Measuring Equine Serum Gastrin Compared to Radioimmunoassay. Animals (Basel), 14(20), 2937. https://doi.org/10.3390/ani14202937

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 14
Issue: 20
PII: 2937

Researcher Affiliations

Vokes, Jessica R
  • School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.
Gedye, Kristene R
  • School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.
Lovett, Amy L
  • School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.
de Kantzow, Max C
  • Department of Agriculture, Fisheries and Forestry, Canberra 2601, Australia.
Shan, Ran
  • Department of Veterinary Clinical Services, The Hong Kong Jockey Club, Conghua Racecourse, Guangzhou 510900, China.
Steel, Catherine M
  • Department of Veterinary Clinical Services, The Hong Kong Jockey Club, Conghua Racecourse, Guangzhou 510900, China.
Sykes, Benjamin W
  • School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.

Grant Funding

  • PPF-2021-102068 / Hong Kong Jockey Club

Conflict of Interest Statement

Ben Sykes has active or recent (last 3 years) consulting engagements with Kelato Animal Health (AUS & USA), A-Vet Health (AUS), Equestra (AUS), Abbey Laboratories (AUS), Troy (AUS), Mayohealthcare (IRE), Fluxergy (USA), and Health Food Symmetry (AUS), all of which have products in the equine gastrointestinal disease space. None of the aforementioned companies had any input into the manuscript. No other authors declare a conflict of interest.

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