Examining the Effects of the Oral Supplement Biota orientalis in the Osteochondral Fragment-Exercise Model of Osteoarthritis in the Horse.
Abstract: Osteoarthritis (OA) is a common problem in horses. Several oral supplements have been proposed as treatments for horses with OA. The object of this study was to evaluate the use of the oil extract from the seeds of (BO) for the treatment of experimentally induced OA in horses. OA was induced in 16, 2-5 year old horses in one middle carpal joint on Day 0; the other limb underwent a sham operation. Once daily oral treatment with BO or placebo was initiated on Day 0 and continued to Day 70. All horses were exercised 5 days a week starting on Day 14 through Day 70. The horses were evaluated every other week for lameness and serum GAG concentration as well as weekly synovial fluid assessment. Magnetic resonance imaging was performed on Day 7 and 70. Radiographic changes were assessed on Day 0, 14, and 70. On Day 70 tissue from the middle carpal joint was assessed macroscopically and histologically. All outcome parameters were compared between treatment groups to identify effects of treatment. Compared to placebo a significant decrease was found in synovial fluid prostaglandin E2 concentration and white blood cell counts in horses treated with BO. There was a significant reduction in radiographic scores for subchondral lysis of the radial carpal bone, osteophyte formation, subchondral sclerosis of the radial carpal bone, and total radiographic score for the horses treated with BO. There was no significant difference between treatment groups in clinical lameness findings, MRI findings, macroscopic grading or histologic grading. This study suggests a significant anti-inflammatory effect from oral BO that should be further investigated in clinical OA.
Copyright © 2022 Seabaugh, Barrett, Rao, McIlwraith and Frisbie.
Publication Date: 2022-06-01 PubMed ID: 35720848PubMed Central: PMC9198577DOI: 10.3389/fvets.2022.858391Google Scholar: Lookup
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Summary
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The research article explores the impact of using the oil extract from Biota orientalis seeds as a treatment for induced osteoarthritis (OA) in horses. It suggests a substantial anti-inflammatory effect of the treatment, which could be worthy of further investigation for clinical use.
Methodology
- The study involved 16 horses, aged between two and five years, in which osteoarthritis was artificially generated in one of their joints. The other limb underwent a dummy operation for control purposes.
- The daily oral application of Biota orientalis (BO) or a placebo started immediately and continued for 70 days. The horses also partook in five days weekly exercise from the 14th day to the end of the experiment.
- Throughout the process, the horses were assessed every fortnight for lameness and lapses in serum GAG (a key factor in joint health) concentration. Simultaneously, a weekly examination of synovial fluid (the viscous fluid present in the cavities of synovial joints) was done.
- MRI scans were conducted on the seventh and seventieth day, and scientists analysed the changes in radiographic imagery on the zeroth, fourteenth, and seventieth day.
- Scientists inspected the tissue from the middle carpal joint visually (macroscopically) and under a microscope (histologically) on the final day.
Results and findings
- The outcome showed a significant decrease in the synovial fluid’s concentration of a particular prostaglandin (E2), a compound with hormone-like effects, and white blood cell counts amongst the horses treated with BO, suggesting a strong anti-inflammatory response.
- The horses treated with BO also indicated a big reduction in radiographic scores related to the radial carpal bone. Elements such as subchondral lysis (breakdown of bone), osteophyte formation (abnormal growth of bone), and subchondral sclerosis (hardening of bone) were positively impacted.
- No significant difference between the BO and placebo groups were noted in regards to clinical lameness, findings from the MRI scans, macroscopic and histologic grading.
- The research concludes that the oral intake of BO may provide a significant anti-inflammatory effect that could be beneficial in managing OA in horses. This needs to be further validated in a clinical setting for better understanding and applicability.
Cite This Article
APA
Seabaugh KA, Barrett MF, Rao S, McIlwraith CW, Frisbie DD.
(2022).
Examining the Effects of the Oral Supplement Biota orientalis in the Osteochondral Fragment-Exercise Model of Osteoarthritis in the Horse.
Front Vet Sci, 9, 858391.
https://doi.org/10.3389/fvets.2022.858391 Publication
Researcher Affiliations
- Orthopaedic Research Center, C. Wayne McIlwraith Translational Medicine Institute, Colorado State University, Fort Collins, CO, United States.
- Orthopaedic Research Center, C. Wayne McIlwraith Translational Medicine Institute, Colorado State University, Fort Collins, CO, United States.
- Department of Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Orthopaedic Research Center, C. Wayne McIlwraith Translational Medicine Institute, Colorado State University, Fort Collins, CO, United States.
- Orthopaedic Research Center, C. Wayne McIlwraith Translational Medicine Institute, Colorado State University, Fort Collins, CO, United States.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
This article includes 30 references
- McIlwraith CW. Traumatic arthritis and posttraumatic osteoarthritis in the horse. In: Mcllwraith W, Frisbie DD, Kawcak CE, van Weeren PR. editors. Joint Disease Horse. Orlando, FL: Elsevier; (2016). p. 33–48.
- Oke SL, McIlwraith CW. Review of the economic impact of osteoarthritis and oral joint-health supplements in horses. Proc AAEP Annu Conf (2010) 56:12–8.
- Caron JP, Genovese RL. Principles and practices of joint disease treatment. In: Ross MW, Dyson SJ. editors. Diagnosis and Management of Lameness in the Horse. Orlando, FL: Elsevier; (2003). p. 746–64.
- Sankaranarayanan A. Nutraceuticals in equine medicine. In: Gupta RC, Srivastava A, Lall R. editors. Nutraceuticals in Veterinary Medicine. Cham: Springer International Publishing; (2019). p. 649–55.
- Swirsley N, Spooner HS, Hoffman RM. Supplement use and perceptions: a study of US horse owners. J Equine Vet Sci (2017) 59:34–9.
- Burk A, Williams C. Feeding management practices and supplement use in top-level event horses. Comp Exerc Physiol (2008) 5:85–93.
- Pearson W, Orth MW, Karrow NA, Lindinger MI. Effects of simulated digests of Biota orientalis and a dietary nutraceutical on interleukin-1- induced inflammatory responses in cartilage explants.. Am J Vet Res 2008 Dec;69(12):1560-8.
- Pearson W, Orth MW, Lindinger MI. Evaluation of inflammatory responses induced via intra-articular injection of interleukin-1 in horses receiving a dietary nutraceutical and assessment of the clinical effects of long-term nutraceutical administration.. Am J Vet Res 2009 Jul;70(7):848-61.
- Ikeda I, Oka T, Koba K, Sugano M, Lie Ken Jie MS. 5c,11c,14c-eicosatrienoic acid and 5c,11c,14c,17c-eicosatetraenoic acid of Biota orientalis seed oil affect lipid metabolism in the rat.. Lipids 1992 Jul;27(7):500-4.
- Beths T, Munn R, Bauquier SH, Mitchell P, Whittem T. A pilot study of 4CYTE™ Epiitalis® Forte, a novel nutraceutical, in the management of naturally occurring osteoarthritis in dogs.. Aust Vet J 2020 Dec;98(12):591-595.
- Kester W. Definition and Classification of Lameness. Guide for Veterinary Services and Judging of Equestrian Events. (1991). p. 19.
- Frisbie DD, Ghivizzani SC, Robbins PD, Evans CH, McIlwraith CW. Treatment of experimental equine osteoarthritis by in vivo delivery of the equine interleukin-1 receptor antagonist gene.. Gene Ther 2002 Jan;9(1):12-20.
- Frisbie DD, Kawcak CE, Trotter GW, Powers BE, Walton RM, McIlwraith CW. Effects of triamcinolone acetonide on an in vivo equine osteochondral fragment exercise model.. Equine Vet J 1997 Sep;29(5):349-59.
- King MR, Haussler KK, Kawcak CE, McIlwraith CW, Reiser RF 2nd, Frisbie DD, Werpy NM. Biomechanical and histologic evaluation of the effects of underwater treadmill exercise on horses with experimentally induced osteoarthritis of the middle carpal joint.. Am J Vet Res 2017 May;78(5):558-569.
- Donnell JR, Frisbie DD, King MR, Goodrich LR, Haussler KK. Comparison of subjective lameness evaluation, force platforms and an inertial-sensor system to identify mild lameness in an equine osteoarthritis model.. Vet J 2015 Nov;206(2):136-42.
- Frisbie DD, McIlwraith CW, Kawcak CE, Werpy NM. Efficacy of intravenous administration of hyaluronan, sodium chondroitin sulfate, and N-acetyl-d-glucosamine for prevention or treatment of osteoarthritis in horses.. Am J Vet Res 2016 Oct;77(10):1064-70.
- Zanotto GM, Liesbeny P, Barrett M, Zlotnick H, Frank E, Grodzinsky AJ, Frisbie DD. Microfracture Augmentation With Trypsin Pretreatment and Growth Factor-Functionalized Self-assembling Peptide Hydrogel Scaffold in an Equine Model.. Am J Sports Med 2021 Jul;49(9):2498-2508.
- Carroll GJ. Spectrophotometric measurement of proteoglycans in osteoarthritic synovial fluid.. Ann Rheum Dis 1987 May;46(5):375-9.
- Frisbie DD, Kawcak CE, Werpy NM, Park RD, McIlwraith CW. Clinical, biochemical, and histologic effects of intra-articular administration of autologous conditioned serum in horses with experimentally induced osteoarthritis.. Am J Vet Res 2007 Mar;68(3):290-6.
- McIlwraith CW, Frisbie DD, Kawcak CE, Fuller CJ, Hurtig M, Cruz A. The OARSI histopathology initiative - recommendations for histological assessments of osteoarthritis in the horse.. Osteoarthritis Cartilage 2010 Oct;18 Suppl 3:S93-105.
- Kim YJ, Sah RL, Doong JY, Grodzinsky AJ. Fluorometric assay of DNA in cartilage explants using Hoechst 33258.. Anal Biochem 1988 Oct;174(1):168-76.
- Kawcak CE, Frisbie DD, McIlwraith CW, Werpy NM, Park RD. Evaluation of avocado and soybean unsaponifiable extracts for treatment of horses with experimentally induced osteoarthritis.. Am J Vet Res 2007 Jun;68(6):598-604.
- Frisbie DD, Al-Sobayil F, Billinghurst RC, Kawcak CE, McIlwraith CW. Changes in synovial fluid and serum biomarkers with exercise and early osteoarthritis in horses.. Osteoarthritis Cartilage 2008 Oct;16(10):1196-204.
- Kirker-Head CA, Chandna VK, Agarwal RK, Morris EA, Tidwell A, O'Callaghan MW, Rand W, Kumar MS. Concentrations of substance P and prostaglandin E2 in synovial fluid of normal and abnormal joints of horses.. Am J Vet Res 2000 Jun;61(6):714-8.
- May SA, Hooke RE, Peremans KY, Verschooten F, Lees P. Prostaglandin-E(2) in Equine Joint Disease. Vlaams Diergeneeskd Tijdschr (1994) 63:187–91.
- Trumble TN, Billinghurst RC, McIlwraith CW. Correlation of prostaglandin E2 concentrations in synovial fluid with ground reaction forces and clinical variables for pain or inflammation in dogs with osteoarthritis induced by transection of the cranial cruciate ligament.. Am J Vet Res 2004 Sep;65(9):1269-75.
- Steel CM. Equine synovial fluid analysis.. Vet Clin North Am Equine Pract 2008 Aug;24(2):437-54, viii.
- Temple-Wong MM, Raleigh AR, Frisbie DD, Sah RL, McIlwraith CW. Effects of an articular cartilage lubrication with a viscosupplement in vitro and in vivo following osteochondral fractures in horses.. Am J Vet Res 2021 Aug;82(8):611-618.
- Richards L, Alexander J, Knight C, Tyrell D, Whittem T. A randomised controlled masked clinical trial of a novel nutraceutical for treatment of osteoarthritis in the dog. J Vet Int Med (2013) 27:750–1.
- Seabaugh KA, Frisbie DD, Barrett MF, McIlwraith CW. Examining the effects of an extract of Biota orientalis in the osteochondral fragment-exercise model of osteoarthritis. In: Proceedings of the Annual Meeting of the American Association of Equine Practitioners. Fort Collins, CO; (2020). p. 455.
Citations
This article has been cited 4 times.- Thampi P, Seabaugh KA, Pezzanite LM, Chu CR, Phillips JN, Grieger JC, McIlwraith CW, Samulski RJ, Goodrich LR. A pilot study to determine the optimal dose of scAAVIL-1ra in a large animal model of post-traumatic osteoarthritis. Gene Ther 2023 Sep 11;.
- Mitchell PG, Bright CA, Bright DR, Srivastava SN, Raote SS, Kumar S. The Biota orientalis, oil extract Epiitalis(®), is efficacious at reducing the symptoms of knee osteoarthritis: a pilot, multi-site, dose-ranging, randomized, blinded, placebo-controlled trial. Inflammopharmacology 2022 Aug;30(4):1323-1334.
- Luedke LK, Seabaugh KA, Cooper BG, Snyder BD, Wimmer MA, McIlwraith CW, Barrett MF, Kawcak CE, Grinstaff MW, Goodrich LR. A Safety and Efficacy Study of a Synthetic Biolubricant in an Equine Model of Post-Traumatic Osteoarthritis. Animals (Basel) 2025 Feb 1;15(3).
- O'Brien TJ, Hollinshead F, Goodrich LR. Extracellular vesicles in the treatment and prevention of osteoarthritis: can horses help us translate this therapy to humans?. Extracell Vesicles Circ Nucl Acids 2023 Jun;4(2):151-169.
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