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Frontiers in veterinary science2025; 11; 1478350; doi: 10.3389/fvets.2024.1478350

Exploring factors that influence the behavior response to novel object tests in young thoroughbred horses: investigating sex, test site and auction history.

Abstract: The novel object test is one of the three most common fear tests in veterinary science and employed in several different species. Although having been applied in several different studies in horses, it is surprising that there is no standardized test procedure available for these kinds of tests. Unassigned: This study investigated the performance of the novel object test on 42 young Thoroughbred horses to determine the effect of sex (mare or stallion), test sites (round pen or paddock) and whether the horses had previously participated in an auction or not on the behavior during the novel object test. Unassigned: Differences in horses' behavior during the novel object test were primarily attributed to the test sites. The animals showed significant ( < 0.05) intra-individual differences in the novel object test in the round pen and in the paddock. Sex did not affect the direct interaction with the novel object. The horses that had not participated in an auction seemed to actively perceive the novel object more quickly, so that the latency to first fixation on the object was significantly shorter. Unassigned: In order to obtain comparable results, it is recommended that novel object tests should be performed at the same location and under identical conditions. Furthermore, it is important to consider the individual behavior of each horse.
Publication Date: 2025-01-15 PubMed ID: 39881719PubMed Central: PMC11776089DOI: 10.3389/fvets.2024.1478350Google Scholar: Lookup
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  • Journal Article

Summary

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This research studies the influence of different conditions including sex, location, and previous auction participation on the behavior of young Thoroughbred horses during a novel object test.

Methodology and Study Parameters

  • The study conducted novel object test on a sample of 42 young Thoroughbred horses.
  • These tests aimed to understand if the behavioral response was influenced by the horse’s sex, the location of the test (either a round pen or a paddock), and the horse’s previous experience of participating in an auction.
  • The novel object test is a widely used fear test in veterinary science, applied to different species to identify their response to an unfamiliar object.
  • This tool’s usage is extensive; however, the lack of a standardized test procedure prompted this research to understand the factors influencing the test results.

Key Findings

  • The primary determinant for the horses’ behavior in the novel object test was identified as the site of the test.
  • Significant intra-individual differences were found in the test results in the round pen versus the paddock settings. This indicates that the environment significantly influences the horse’s behavior during the test.
  • The sex of the horse did not exhibit any significant impact on the interaction with the novel object indicating that gender does not play a role in the horse’s response to unfamiliar items.
  • Horses that had not previously participated in auctions actively perceived the novel object more quickly. This resulted in significantly shorter latencies to the first fixation on the object, suggesting that previous exposure to stressful scenarios such as auctions might affect behavior.

Recommendations

  • The results suggest that novel object tests should be conducted under uniform conditions in identical locations for obtaining comparable results. Factors like the test environment can influence the animal’s behaviors, hence controlling these factors will ensure more reliable outcomes.
  • The research also recommends consideration of the individual behaviors of each horse, implying the need to account for the horse’s personal history, like previous exposure to auctions for accurate test readings.

Cite This Article

APA
Klitzing L, Kirsch K, Schindler M, Merle R, Hoffmann G, Thöne-Reineke C, Wiegard M. (2025). Exploring factors that influence the behavior response to novel object tests in young thoroughbred horses: investigating sex, test site and auction history. Front Vet Sci, 11, 1478350. https://doi.org/10.3389/fvets.2024.1478350

Publication

ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 11
Pages: 1478350
PII: 1478350

Researcher Affiliations

Klitzing, Lara
  • Institute for Animal Welfare, Animal Behavior and Laboratory Animal Science, Freie Universität Berlin, Berlin, Germany.
Kirsch, Katharina
  • Institute for Animal Welfare, Animal Behavior and Laboratory Animal Science, Freie Universität Berlin, Berlin, Germany.
Schindler, Maria
  • Institute for Animal Welfare, Animal Behavior and Laboratory Animal Science, Freie Universität Berlin, Berlin, Germany.
Merle, Roswitha
  • Institute for Veterinary Epidemiology and Biostatistics, Freie Universität Berlin, Berlin, Germany.
Hoffmann, Gundula
  • Department Sensors and Modelling, Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Potsdam, Germany.
Thöne-Reineke, Christa
  • Institute for Animal Welfare, Animal Behavior and Laboratory Animal Science, Freie Universität Berlin, Berlin, Germany.
Wiegard, Mechthild
  • Institute for Animal Welfare, Animal Behavior and Laboratory Animal Science, Freie Universität Berlin, Berlin, Germany.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.

References

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Citations

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