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Expression and immunolocalisation of follicle-stimulating hormone receptors in gonads of newborn and adult female horses.
Abstract: In mares, FSH and its receptor (FSHR) are essential for ovarian function. The objective of the present study was to analyse FSHR gene expression at the mRNA and protein levels in ovarian tissue from newborn and adult horses. Expression of mRNA was analysed by reverse transcription polymerase chain reaction, whereas FSHR protein was visualised by immunohistochemistry (IHC), immunofluorescence labelling (IF) and western blot. FSHR mRNA was detected in ovarian follicles and luteal tissue from adult mares, as well as in the ovaries of neonates. Follicular growth up to 4mm in diameter was already present in neonates. Using IHC and IF, FSHR protein was detected in granulosa cells, cumulus cells and inconsistently in oocytes, independent of the animal's age or the stage of folliculogenesis. A lower FSHR expression was observed in theca cells in comparison to granulosa cells. FSHR was abundant in the ovarian stroma cells of neonates but not of adults. Luteal cells stained positive for FSHR independent of the stage of corpus luteum development. The presence of FSHR protein in various cell populations of the ovary was confirmed by western blot. In conclusion, FSHR is present in horse ovaries consistently from birth onwards and expression remains constant during the oestrous cycle.
Publication Date: 2015-02-19 PubMed ID: 25693905DOI: 10.1071/RD14392Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The study analyzes the expression of Follicle-stimulating hormone receptors (FSHR) in newborn and adult female horses to understand its role in ovarian function. The research shows that FSHR is present in horse ovaries from birth and its expression is consistent throughout different stages of the oestrous cycle.
Objectives and Methodology of the Study
- The main goal of this study was to inspect the presence and level of FSHR gene expression in the ovarian tissues of newborn and adult horses.
- Through techniques such as reverse transcription polymerase chain reaction, the expression of FSHR mRNA was observed.
- Other methods such as immunohistochemistry (IHC), immunofluorescence labelling (IF), and western blot were used to visualize the FSHR protein.
Findings of the Study
- Expression of the FSHR mRNA was detected in ovarian follicles, luteal tissue of adult mares, as well as in the ovaries of neonates. Notably, follicular growth of up to 4mm was already detected in the ovaries of newborn horses.
- The results showed that FSHR protein can also be found in granulosa cells, cumulus cells, and even inconsistently in the oocytes, regardless of the horse’s age or the stage of folliculogenesis. This implies that FSHR plays a significant role across various ovarian cells and developmental stages.
- The study found that FSHR expression in theca cells was lower compared to that in granulosa cells.
- Interestingly, FSHR was found to be abundant in the ovarian stroma cells of neonates but not adults. Furthermore, luteal cells tested positive for FSHR, independent of the stage of corpus luteum development.
- The researchers confirmed the presence of FSHR protein across various ovarian cell populations through western blotting.
Conclusion of the Study
- The research confirmed that FSHR is present in horse ovaries from birth and continues to express consistently throughout the oestrous cycle, thereby suggesting a constant role in ovarian function.
Cite This Article
APA
Scarlet D, Walter I, Hlavaty J, Aurich C.
(2015).
Expression and immunolocalisation of follicle-stimulating hormone receptors in gonads of newborn and adult female horses.
Reprod Fertil Dev.
https://doi.org/10.1071/RD14392 Publication
Researcher Affiliations
Citations
This article has been cited 8 times.- Jitjumnong J, Tang PC. Bone Morphogenetic Protein 15 (BMP-15) Improves In Vitro Mouse Folliculogenesis. Animals (Basel) 2023 Mar 8;13(6).
- Zhang X, Dong C, Yang J, Li Y, Feng J, Wang B, Zhang J, Guo X. The Roles of the miRNAome and Transcriptome in the Ovine Ovary Reveal Poor Efficiency in Juvenile Superovulation. Animals (Basel) 2021 Jan 19;11(1).
- Ishak GM, Bashir ST, Dutra GA, Gastal GDA, Gastal MO, Cavinder CA, Feugang JM, Gastal EL. In vivo antral follicle wall biopsy: a new research technique to study ovarian function at the cellular and molecular levels. Reprod Biol Endocrinol 2018 Jul 28;16(1):71.
- Shoorei H, Khaki A, Ainehchi N, Hassanzadeh Taheri MM, Tahmasebi M, Seyedghiasi G, Ghoreishi Z, Shokoohi M, Khaki AA, Abbas Raza SH. Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System. Chin Med J (Engl) 2018 Jan 20;131(2):218-225.
- Salehnia M, Pajokh M, Ghorbanmehr N. Short Term Organ Culture of Mouse Ovary in the Medium Supplemented with Bone Morphogenetic Protein 15 and Follicle Stimulating Hormone: A Morphological, Hormonal and Molecular Study. J Reprod Infertil 2016 Oct-Dec;17(4):199-207.
- Scarlet D, Ertl R, Aurich C, Steinborn R. The Orthology Clause in the Next Generation Sequencing Era: Novel Reference Genes Identified by RNA-seq in Humans Improve Normalization of Neonatal Equine Ovary RT-qPCR Data. PLoS One 2015;10(11):e0142122.
- Walter J, Colleoni S, Lazzari G, Fortes C, Grossmann J, Roschitzki B, Laczko E, Naegeli H, Bleul U, Galli C. Maturational competence of equine oocytes is associated with alterations in their 'cumulome'. Mol Hum Reprod 2024 Sep 12;30(9).
- Shoorei H, Jafarabadi M, PourBayranvand S, Salehnia M. Comparison of mouse ovarian follicular development and gene expression in the presence of ovarian tissue extract and sodium selenite: An experimental study. Int J Reprod Biomed 2023 May;21(5):415-424.
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