Expression of anti-Müllerian hormone (AMH) in equine granulosa-cell tumors and in normal equine ovaries.
Abstract: Anti-Müllerian hormone (AMH), also known as Müllerian inhibiting substance (MIS), is expressed by granulosa cells in females of many mammalian species, and circulating AMH concentrations have been used to monitor granulosa-cell tumors (GCT) in women. The objective was to characterize expression of AMH in equine GCT, and in normal equine ovaries, based upon immunohistochemistry (IHC), using a polyclonal primary antibody directed against human AMH. Equine GCT (n=27) and normal equine ovaries (n=10) were examined by IHC. In addition, sera from four mares with GCT were characterized for AMH bioactivity, based upon suppression of Müllerian duct development in the fetal rat. Immunolabeling with alpha-AMH was localized to granulosa cells in equine GCT, as well as within antral follicles in normal ovaries. Expression of AMH first appeared in granulosa cells of small growing follicles and was most intense in small antral follicles; large antral or atretic follicles had reduced immunolabeling. Omission of the primary antibody or incubation of the primary antibody with the corresponding blocking peptide eliminated immunolabeling of granulosa cells in GCT and in normal antral follicles, confirming the specificity of the immunolabel. Sera from mares with GCT had increased AMH bioactivity compared to control sera. In conclusion, AMH was strongly expressed by granulosa cells in equine GCT and in normal antral follicles. Therefore, anti-Müllerian hormone may be a useful biomarker for detection of GCT in the horse.
Publication Date: 2008-07-03 PubMed ID: 18599114DOI: 10.1016/j.theriogenology.2008.05.059Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigates the expression of Anti-Müllerian hormone (AMH) in equine granulosa-cell tumors and normal equine ovaries, with the results suggesting that AMH could possibly serve as a biomarker for detecting granulosa-cell tumors in horses.
Objective and Methodology
- The primary objective of the research was to evaluate the expression of Anti-Müllerian hormone (AMH) in equine granulosa-cell tumors (GCT) and in normal equine ovaries.
- This was approached using immunohistochemistry (IHC) and a polyclonal primary antibody against human AMH.
- The study examined 27 equine GCT and 10 normal equine ovaries using IHC.
- In addition, sera from four mares with GCT were analyzed for AMH bioactivity, based on the suppression of Müllerian duct development in the fetal rat.
Results
- Immunolabeling confirmed the presence of alpha-AMH in the granulosa cells of equine GCT, as well as within antral follicles in normal ovaries.
- AMH expression was first observed in granulosa cells of small growing follicles and was most intense in small antral follicles; large antral or atretic follicles showed decreased immunolabeling.
- Tests wherein the primary antibody was omitted or incubated with the corresponding blocking peptide eradicated the immunolabeling of granulosa cells in GCT and in normal antral follicles. This confirms the specificity of the immunolabel.
- Sera samples from mares with GCT showed increased AMH bioactivity compared to control sera.
Conclusion
- AMH was found to be substantially expressed by granulosa cells in equine GCT and in normal antral follicles.
- The research concludes that Anti-Müllerian hormone could potentially serve as a useful biomarker for detecting GCT in horses.
Cite This Article
APA
Ball BA, Conley AJ, MacLaughlin DT, Grundy SA, Sabeur K, Liu IK.
(2008).
Expression of anti-Müllerian hormone (AMH) in equine granulosa-cell tumors and in normal equine ovaries.
Theriogenology, 70(6), 968-977.
https://doi.org/10.1016/j.theriogenology.2008.05.059 Publication
Researcher Affiliations
- Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, United States. baball@ucdavis.edu
MeSH Terms
- Animals
- Anti-Mullerian Hormone / blood
- Anti-Mullerian Hormone / metabolism
- Female
- Granulosa Cell Tumor / blood
- Granulosa Cell Tumor / metabolism
- Granulosa Cell Tumor / pathology
- Horses / blood
- Horses / metabolism
- Inhibins / blood
- Inhibins / metabolism
- Ovarian Neoplasms / blood
- Ovarian Neoplasms / metabolism
- Ovarian Neoplasms / pathology
- Ovary / metabolism
- Ovary / pathology
Citations
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