Expression of cell-surface antigens and embryonic stem cell pluripotency genes in equine blastocysts.
Abstract: Embryonic stem-like (ES-like) cells have now been derived from the inner cell mass (ICM) of horse embryos at the blastocyst stage. Because they have been shown to express cell-surface antigens found in both human and mouse ES cells, the present study investigated gene expression patterns in day-7 horse blastocysts from which the horse ES-like cells had been derived originally. The genes studied included Oct-4, stage-specific embryonic antigen-1 (SSEA-1), SSEA-3, SSEA-4, tumor rejection antigen-1-60 (TRA-1-60), TRA-1-81, and alkaline phosphatase activity, and whereas all three of the SSEA antigens were expressed in both the ICM and the trophoblast on day 7, Oct-4, TRA-1-60, TRA-1-81, and alkaline phosphatase activity were localized mostly in the ICM. Upon in vitro differentiation of the horse ES-like cells, their expression of the stem cell markers was abolished. Therefore, the species-specific expression pattern of stem cell markers in horse ES-like cells reflects gene expression in the blastocysts from which they are derived.
Publication Date: 2007-11-15 PubMed ID: 17999600DOI: 10.1089/scd.2007.0032Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates the gene expression patterns in horse blastocysts, specifically in relation to embryonic stem-like (ES-like) cells. The study finds that certain pluripotency genes and cell-surface antigens present in human and mouse stem cells are also expressed in these horse cells, mainly concentrated in the inner cell mass (ICM).
Objective of the Research
The purpose of this research was to examine the expression of cell-surface antigens and pluripotency genes in the embryonic stem-like (ES-like) cells extracted from equine (horse) blastocysts.
- The object of study, horse blastocysts, are embryos in the early stages of development.
- The focus lies on the inner cell mass (ICM) of these blastocysts, where ES-like cells are located.
- The research aimed to identify patterns in the gene expression of these cells and compare them to patterns seen in human and mouse ES cells.
Pluripotency Genes and Cell-Surface Antigens
A significant component of the research involved investigating the expression of certain pluripotency genes and cell-surface antigens.
- Included among these were Oct-4, stage-specific embryonic antigen-1 (SSEA-1), SSEA-3, SSEA-4, tumor rejection antigen-1-60 (TRA-1-60), TRA-1-81, and indicators of alkaline phosphatase activity.
- While SSEA antigens were found both in the ICM and trophoblast of the day-7 blastocysts, Oct-4, TRA-1-60, TRA-1-81, and alkaline phosphatase activity were primarily present in the ICM of the embryonic structure.
Results and Implications
The research revealed that upon in vitro differentiation of horse ES-like cells, their expression of the stem cell markers ceased.
- This suggests that the expression pattern of stem cell markers in horse ES-like cells is indicative of gene expression within the originating blastocysts.
- The study therefore helps to establish a clear link between ES-like cells and the blastocysts from which they are derived. This further underscores the relevance and potential applications of ES-like cells in scientific and medical research.
Cite This Article
APA
Guest DJ, Allen WR.
(2007).
Expression of cell-surface antigens and embryonic stem cell pluripotency genes in equine blastocysts.
Stem Cells Dev, 16(5), 789-796.
https://doi.org/10.1089/scd.2007.0032 Publication
Researcher Affiliations
- Department of Veterinary Medicine Equine Fertility Unit, University of Cambridge, Newmarket, Suffolk, CB8 9BH, UK. debbie.guest@aht.org.uk
MeSH Terms
- Animals
- Antigens, Surface / genetics
- Antigens, Surface / metabolism
- Biomarkers / metabolism
- Blastocyst / cytology
- Blastocyst / metabolism
- Cell Differentiation
- Embryonic Stem Cells / cytology
- Embryonic Stem Cells / metabolism
- Eosine Yellowish-(YS)
- Equidae / genetics
- Gene Expression Regulation
- Hematoxylin
- Immunohistochemistry
- Organ Specificity
- Pluripotent Stem Cells / metabolism
Citations
This article has been cited 20 times.- Arzi B, Webb TL, Koch TG, Volk SW, Betts DH, Watts A, Goodrich L, Kallos MS, Kol A. Cell Therapy in Veterinary Medicine as a Proof-of-Concept for Human Therapies: Perspectives From the North American Veterinary Regenerative Medicine Association.. Front Vet Sci 2021;8:779109.
- Hisey E, Ross PJ, Meyers SA. A Review of OCT4 Functions and Applications to Equine Embryos.. J Equine Vet Sci 2021 Mar;98:103364.
- Paterson YZ, Cribbs A, Espenel M, Smith EJ, Henson FMD, Guest DJ. Genome-wide transcriptome analysis reveals equine embryonic stem cell-derived tenocytes resemble fetal, not adult tenocytes.. Stem Cell Res Ther 2020 May 19;11(1):184.
- McClellan A, Evans R, Sze C, Kan S, Paterson Y, Guest D. A novel mechanism for the protection of embryonic stem cell derived tenocytes from inflammatory cytokine interleukin 1 beta.. Sci Rep 2019 Feb 26;9(1):2755.
- Moro LN, Amin G, Furmento V, Waisman A, Garate X, Neiman G, La Greca A, Santín Velazque NL, Luzzani C, Sevlever GE, Vichera G, Miriuka SG. MicroRNA characterization in equine induced pluripotent stem cells.. PLoS One 2018;13(12):e0207074.
- Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance.. Cell Transplant 2018 Jan;27(1):93-116.
- Orsztynowicz M, Lechniak D, Pawlak P, Kociucka B, Kubickova S, Cernohorska H, Madeja ZE. Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification.. PLoS One 2017;12(8):e0182398.
- Hashimoto S, Morimoto N, Yamanaka M, Matsumoto H, Yamochi T, Goto H, Inoue M, Nakaoka Y, Shibahara H, Morimoto Y. Quantitative and qualitative changes of mitochondria in human preimplantation embryos.. J Assist Reprod Genet 2017 May;34(5):573-580.
- Bavin EP, Smith O, Baird AE, Smith LC, Guest DJ. Equine Induced Pluripotent Stem Cells have a Reduced Tendon Differentiation Capacity Compared to Embryonic Stem Cells.. Front Vet Sci 2015;2:55.
- Alipour F, Parham A, Kazemi Mehrjerdi H, Dehghani H. Equine adipose-derived mesenchymal stem cells: phenotype and growth characteristics, gene expression profile and differentiation potentials.. Cell J 2015 Winter;16(4):456-65.
- Ulrich H, do Nascimento IC, Bocsi J, Tárnok A. Immunomodulation in stem cell differentiation into neurons and brain repair.. Stem Cell Rev Rep 2015 Jun;11(3):474-86.
- Paterson YZ, Rash N, Garvican ER, Paillot R, Guest DJ. Equine mesenchymal stromal cells and embryo-derived stem cells are immune privileged in vitro.. Stem Cell Res Ther 2014 Jul 30;5(4):90.
- Barsby T, Bavin EP, Guest DJ. Three-dimensional culture and transforming growth factor beta3 synergistically promote tenogenic differentiation of equine embryo-derived stem cells.. Tissue Eng Part A 2014 Oct;20(19-20):2604-13.
- Guest DJ, Ousey JC, Smith MR. Defining the expression of marker genes in equine mesenchymal stromal cells.. Stem Cells Cloning 2008;1:1-9.
- Madeja ZE, Sosnowski J, Hryniewicz K, Warzych E, Pawlak P, Rozwadowska N, Plusa B, Lechniak D. Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development.. BMC Dev Biol 2013 Aug 13;13:32.
- Kumar R, Ahlawat SP, Sharma M, Verma OP, Sai Kumar G, Taru Sharma G. Selection of appropriate isolation method based on morphology of blastocyst for efficient derivation of buffalo embryonic stem cells.. Cytotechnology 2014 Mar;66(2):239-50.
- Spaas JH, Guest DJ, Van de Walle GR. Tendon regeneration in human and equine athletes: Ubi Sumus-Quo Vadimus (where are we and where are we going to)?. Sports Med 2012 Oct 1;42(10):871-90.
- Breton A, Sharma R, Diaz AC, Parham AG, Graham A, Neil C, Whitelaw CB, Milne E, Donadeu FX. Derivation and characterization of induced pluripotent stem cells from equine fibroblasts.. Stem Cells Dev 2013 Feb 15;22(4):611-21.
- Hackett CH, Fortier LA. Embryonic stem cells and iPS cells: sources and characteristics.. Vet Clin North Am Equine Pract 2011 Aug;27(2):233-42.
- Facucho-Oliveira JM, St John JC. The relationship between pluripotency and mitochondrial DNA proliferation during early embryo development and embryonic stem cell differentiation.. Stem Cell Rev Rep 2009 Jun;5(2):140-58.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
