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Stem cells and development2007; 16(5); 789-796; doi: 10.1089/scd.2007.0032

Expression of cell-surface antigens and embryonic stem cell pluripotency genes in equine blastocysts.

Abstract: Embryonic stem-like (ES-like) cells have now been derived from the inner cell mass (ICM) of horse embryos at the blastocyst stage. Because they have been shown to express cell-surface antigens found in both human and mouse ES cells, the present study investigated gene expression patterns in day-7 horse blastocysts from which the horse ES-like cells had been derived originally. The genes studied included Oct-4, stage-specific embryonic antigen-1 (SSEA-1), SSEA-3, SSEA-4, tumor rejection antigen-1-60 (TRA-1-60), TRA-1-81, and alkaline phosphatase activity, and whereas all three of the SSEA antigens were expressed in both the ICM and the trophoblast on day 7, Oct-4, TRA-1-60, TRA-1-81, and alkaline phosphatase activity were localized mostly in the ICM. Upon in vitro differentiation of the horse ES-like cells, their expression of the stem cell markers was abolished. Therefore, the species-specific expression pattern of stem cell markers in horse ES-like cells reflects gene expression in the blastocysts from which they are derived.
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Publication Date: 2007-11-15 PubMed ID: 17999600DOI: 10.1089/scd.2007.0032Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the gene expression patterns in horse blastocysts, specifically in relation to embryonic stem-like (ES-like) cells. The study finds that certain pluripotency genes and cell-surface antigens present in human and mouse stem cells are also expressed in these horse cells, mainly concentrated in the inner cell mass (ICM).

Objective of the Research

The purpose of this research was to examine the expression of cell-surface antigens and pluripotency genes in the embryonic stem-like (ES-like) cells extracted from equine (horse) blastocysts.

  • The object of study, horse blastocysts, are embryos in the early stages of development.
  • The focus lies on the inner cell mass (ICM) of these blastocysts, where ES-like cells are located.
  • The research aimed to identify patterns in the gene expression of these cells and compare them to patterns seen in human and mouse ES cells.

Pluripotency Genes and Cell-Surface Antigens

A significant component of the research involved investigating the expression of certain pluripotency genes and cell-surface antigens.

  • Included among these were Oct-4, stage-specific embryonic antigen-1 (SSEA-1), SSEA-3, SSEA-4, tumor rejection antigen-1-60 (TRA-1-60), TRA-1-81, and indicators of alkaline phosphatase activity.
  • While SSEA antigens were found both in the ICM and trophoblast of the day-7 blastocysts, Oct-4, TRA-1-60, TRA-1-81, and alkaline phosphatase activity were primarily present in the ICM of the embryonic structure.

Results and Implications

The research revealed that upon in vitro differentiation of horse ES-like cells, their expression of the stem cell markers ceased.

  • This suggests that the expression pattern of stem cell markers in horse ES-like cells is indicative of gene expression within the originating blastocysts.
  • The study therefore helps to establish a clear link between ES-like cells and the blastocysts from which they are derived. This further underscores the relevance and potential applications of ES-like cells in scientific and medical research.

Cite This Article

APA
Guest DJ, Allen WR. (2007). Expression of cell-surface antigens and embryonic stem cell pluripotency genes in equine blastocysts. Stem Cells Dev, 16(5), 789-796. https://doi.org/10.1089/scd.2007.0032

Publication

Stem cells and development
ISSN: 1547-3287
NlmUniqueID: 101197107
Country: United States
Language: English
Volume: 16
Issue: 5
Pages: 789-796

Researcher Affiliations

Guest, D J
  • Department of Veterinary Medicine Equine Fertility Unit, University of Cambridge, Newmarket, Suffolk, CB8 9BH, UK. debbie.guest@aht.org.uk
Allen, W R

    MeSH Terms

    • Animals
    • Antigens, Surface / genetics
    • Antigens, Surface / metabolism
    • Biomarkers / metabolism
    • Blastocyst / cytology
    • Blastocyst / metabolism
    • Cell Differentiation
    • Embryonic Stem Cells / cytology
    • Embryonic Stem Cells / metabolism
    • Eosine Yellowish-(YS)
    • Equidae / genetics
    • Gene Expression Regulation
    • Hematoxylin
    • Immunohistochemistry
    • Organ Specificity
    • Pluripotent Stem Cells / metabolism

    Citations

    This article has been cited 23 times.
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