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BMC veterinary research2013; 9; 240; doi: 10.1186/1746-6148-9-240

Expression of inflammation-related genes is associated with adipose tissue location in horses.

Abstract: In humans, adipose tissue (AT) originating from different depots shows varying gene expression profiles. In horses, the risk of certain metabolic disorders may also be influenced by the impact of specific AT depots. Macrophage infiltration in human and rat AT is considered to be a source of inflammatory changes. In horses, this relationship has not been extensively studied yet. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), a useful method to evaluate differences in mRNA expression across different tissues, can be used to evaluate differences between equine AT depots. For a correct interpretation of the RT-qPCR results, expression data have to be normalized by the use of validated reference genes. The main objectives of this study were to compare mRNA expression of inflammation-related genes, as well as adipocyte morphology and number between different equine AT depots; and in addition, to investigate the presence of antigen presenting cells in equine AT and any potential relationship with adipokine mRNA expression. Results: In this study, the mRNA expression of inflammation-related genes (leptin, chemokine ligand 5, interleukin 1β, interleukin 6, interleukin 10, adiponectin, matrix metalloproteinase 2, and superoxide dismutase 2) and candidate reference gene stability was investigated in 8 different AT depots collected from the nuchal, abdominal (mesenteric, retroperitoneal, and peri-renal) and subcutaneous (tail head and loin) AT region. By using GeNorm analysis, HPRT1, RPL32, and GAPDH were found to be the most stable genes in equine AT. The mRNA expression of leptin, chemokine ligand 5, interleukin 10, interleukin 1β, adiponectin, and matrix metalloproteinase 2 significantly differed across AT depots (P  0.05). Adipocyte area and number of antigen presenting cells per adipocyte significantly differed between AT depots (P < 0.05). Conclusions: Adipose tissue location was associated with differences in mRNA expression of inflammation-related genes. This depot-specific difference in mRNA expression suggests that the overall inflammatory status of horses could be partially determined by the relative proportion of the different AT depots.
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Publication Date: 2013-12-02 PubMed ID: 24295090PubMed Central: PMC4220830DOI: 10.1186/1746-6148-9-240Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines the variation in the expression of inflammation-related genes linked to adipose (fat) tissue location in horses. The study provides insights into how different fat deposits contribute to overall inflammatory status and potentially influence the risk of metabolic disorders in horses.

Objective of the Research

  • The aim of the study was to compare the mRNA expression of inflammation-related genes, as well as examine the morphology and number of adipocytes (fat cells) across different adipose tissue depots in horses.
  • The research also sought to investigate the presence of antigen presenting cells in horse fat deposits, and any correlation with adipokine mRNA expression.

Methodology and Materials

  • Eight different adipose tissue depots including nuchal, abdominal (mesenteric, retroperitoneal, peri-renal), and subcutaneous (tail head and loin) were studied.
  • Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was used to evaluate the differences in mRNA expression of inflammation-related genes across these tissues.
  • To ensure accurate interpretation of the RT-qPCR results, the expression data was normalized using validated reference genes. Through GeNorm analysis, HPRT1, RPL32, and GAPDH were identified as the most stable genes in equine adipose tissue.

Results of the Study

  • The mRNA expression of inflammation-related genes including leptin, chemokine ligand 5, interleukin 1β, interleukin 6, interleukin 10, adiponectin, matrix metalloproteinase 2, and superoxide dismutase 2 was investigated.
  • Significant variance was found in the expression of leptin, chemokine ligand 5, interleukin 10, interleukin 1β, adiponectin, and matrix metalloproteinase 2 across the different adipose tissue depots. However, no significant variances were found for interleukin 6 and superoxide dismutase 2.
  • The study also found significant differences in adipocyte area and number of antigen presenting cells per adipocyte across the different adipose tissue depots.

Conclusions of the Study

  • The location of adipose tissue was associated with differences in mRNA expression of inflammation-related genes in horses. These depots-specific differences suggest that the overall inflammatory status of horses could be partially determined by the relative proportion of the different fat depots.

Cite This Article

APA
Bruynsteen L, Erkens T, Peelman LJ, Ducatelle R, Janssens GP, Harris PA, Hesta M. (2013). Expression of inflammation-related genes is associated with adipose tissue location in horses. BMC Vet Res, 9, 240. https://doi.org/10.1186/1746-6148-9-240

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 9
Pages: 240

Researcher Affiliations

Bruynsteen, Lien
  • Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke 9820, Belgium. lien.bruynsteen@ugent.be.
Erkens, Tim
    Peelman, Luc J
      Ducatelle, Richard
        Janssens, Geert P J
          Harris, Patricia A
            Hesta, Myriam

              MeSH Terms

              • Adipocytes / metabolism
              • Adipose Tissue / metabolism
              • Animals
              • Gene Expression Regulation
              • Horse Diseases / genetics
              • Horse Diseases / metabolism
              • Horses / genetics
              • Horses / metabolism
              • Inflammation / genetics
              • Inflammation / metabolism
              • Inflammation / veterinary
              • Male
              • Reverse Transcriptase Polymerase Chain Reaction / veterinary
              • Transcriptome / genetics

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