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Molecular biology reports2019; 46(2); 2485-2491; doi: 10.1007/s11033-018-04579-5

Expression of interleukins 6 and 10 and population of inflammatory cells in the equine endometrium: diagnostic implications.

Abstract: Endometritis consists of an acute or chronic inflammatory process involving the endometrium and together with endometrosis constitute the main causes of infertility in mares. The aim of this study was to associate the histopathological findings with the immunohistochemical markers interleukins 6 (IL-6) and 10 (IL-10) to evaluate the inflammatory changes and progression of uterine tissue lesions of mares in the diestrus phase and their diagnostic implications. Twelve crossbred cyclic mares were used for endometrial biopsy collection. Samples were collected in the diestrus period (6 ± 1 day after ovulation) without previous artificial insemination. In the histopathological analysis the samples were classified according to the type and intensity of inflammation, alterations regarding endometrial fibrosis and biopsy categories (I, IIA, IIB and III). In the immunohistochemical analysis, the markers of IL-6 and IL-10 were evaluated by scores (0, 2, 4, 6) according to the intensity of the immunostaining and inflammatory cells (CD-3, CD-20, CD-68 and MPO antibodies) and were counted according to the number of cells immunostained in brown, in ten random fields. An association (p ≤ 0.05) occurred between low score (2) for IL-6 in the endometrial glandular area and moderate fibrotic nets; and between high scores (4 and 6) for IL-10 in sub-epithelial connective tissue and moderate periglandular fibrosis. In conclusion, immunohistochemical analysis demonstrated an association between interleukins and inflammatory cells with endometrial lesions. In addition, this research may be useful in the future to evaluate the progress of the inflammatory process, contributing to the adequate optimization of the reproductive management of the mares.
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Publication Date: 2019-03-21 PubMed ID: 30900135DOI: 10.1007/s11033-018-04579-5Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research studied the relationship between the expression of interleukins 6 and 10 and the presence of inflammatory cells in the equine endometrium. Findings confirm a correlation between these factors and endometrial lesions, potentially aiding in future research in understanding reproductive issues in mares.

Introduction and Purpose of the Study

  • The study aimed at investigating the role of interleukin 6 (IL-6) and 10 (IL-10) in endometritis, an inflammatory condition in mares that impacts fertility. Understanding these connections may provide insights to optimize reproductive management of mares.

Methodology

  • The research team used twelve crossbred cyclic mares for endometrial biopsy collection, specifically during the diestrus phase, which is 6 ± 1 day after ovulation.
  • Two key evaluation methods were used: histopathological analysis, to study tissue changes on a microscopic level, and immunohistochemical analysis to determine the presence and measurement of IL-6 and IL-10, as well as inflammatory cells.

Findings

  • Samples analyzed were classified based on the type and intensity of inflammation, alterations in endometrial fibrosis, and by biopsy categories (I, IIA, IIB, III).
  • The study reports an association between a low score of IL-6 in the endometrial glandular area and moderate fibrotic nets. This information suggests that a decreased presence of IL-6 might correlate with an increase in fibrotic changes in mares suffering from endometritis.
  • A high score for IL-10 in the sub-epithelial connective tissue was associated with moderate periglandular fibrosis. This indicates that high secretion of IL-10 may further promote fibrotic changes.

Conclusion

  • This study showed a clear association between the interleukins IL-6 and IL-10 and inflammatory cells with endometrial lesions in mares.
  • Finding such a connection enhances the understanding of the inflammatory processes that result in endometritis and may also assist in the effective management of mare reproduction.

Cite This Article

APA
de Holanda AGB, da Silva Leite J, Consalter A, da Silva KVGC, Dos Santos Batista BP, Fonseca ABM, Brandão FZ, Ferreira AMR. (2019). Expression of interleukins 6 and 10 and population of inflammatory cells in the equine endometrium: diagnostic implications. Mol Biol Rep, 46(2), 2485-2491. https://doi.org/10.1007/s11033-018-04579-5

Publication

Molecular biology reports
ISSN: 1573-4978
NlmUniqueID: 0403234
Country: Netherlands
Language: English
Volume: 46
Issue: 2
Pages: 2485-2491

Researcher Affiliations

de Holanda, Aline Gomes Buarque
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil. alinearaujo@id.uff.br.
da Silva Leite, Juliana
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil.
  • Laboratório de Anatomia Patológica Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Consalter, Angélica
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil.
da Silva, Kassia Valéria Gomes Coelho
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil.
  • Laboratório de Anatomia Patológica Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Dos Santos Batista, Barbara Paula
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil.
Fonseca, Ana Beatriz Monteiro
  • Instituto de Matemática e Estatística, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Brandão, Felipe Zandonadi
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil.
  • Setor de Reprodução Animal, Universidade Federal Fluminense, Niterói, Brazil.
Ferreira, Ana Maria Reis
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil. ana_ferreira@id.uff.br.
  • Laboratório de Anatomia Patológica Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil. ana_ferreira@id.uff.br.

MeSH Terms

  • Animals
  • Biopsy / methods
  • Endometritis / diagnosis
  • Endometritis / immunology
  • Endometritis / metabolism
  • Endometrium / immunology
  • Endometrium / metabolism
  • Female
  • Horses / genetics
  • Horses / immunology
  • Inflammation / genetics
  • Inflammation / immunology
  • Interleukin-10 / analysis
  • Interleukin-10 / genetics
  • Interleukin-6 / analysis
  • Interleukin-6 / genetics
  • Uterus / pathology

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Citations

This article has been cited 5 times.
  1. Zhang W, Yuan Y, Huang G, Xiao J. Potential Molecular Mechanism of Guishen Huoxue Decoction against Intrauterine Adhesion Based on Network Pharmacology. Evid Based Complement Alternat Med 2022;2022:4049147.
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  4. Yi J, Zhang P, Chen L, Chu S, Li L. Diagnostic value of CD20 combined with CD138 positive expression in patients with chronic endometritis. Am J Clin Exp Immunol 2025;14(3):145-156.
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