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Home / Equine Research Bank / Mol Biol Rep / Expression of interleukins 6 and 10 and population of inflam...
Molecular biology reports2019; 46(2); 2485-2491; doi: 10.1007/s11033-018-04579-5

Expression of interleukins 6 and 10 and population of inflammatory cells in the equine endometrium: diagnostic implications.

Abstract: Endometritis consists of an acute or chronic inflammatory process involving the endometrium and together with endometrosis constitute the main causes of infertility in mares. The aim of this study was to associate the histopathological findings with the immunohistochemical markers interleukins 6 (IL-6) and 10 (IL-10) to evaluate the inflammatory changes and progression of uterine tissue lesions of mares in the diestrus phase and their diagnostic implications. Twelve crossbred cyclic mares were used for endometrial biopsy collection. Samples were collected in the diestrus period (6 ± 1 day after ovulation) without previous artificial insemination. In the histopathological analysis the samples were classified according to the type and intensity of inflammation, alterations regarding endometrial fibrosis and biopsy categories (I, IIA, IIB and III). In the immunohistochemical analysis, the markers of IL-6 and IL-10 were evaluated by scores (0, 2, 4, 6) according to the intensity of the immunostaining and inflammatory cells (CD-3, CD-20, CD-68 and MPO antibodies) and were counted according to the number of cells immunostained in brown, in ten random fields. An association (p ≤ 0.05) occurred between low score (2) for IL-6 in the endometrial glandular area and moderate fibrotic nets; and between high scores (4 and 6) for IL-10 in sub-epithelial connective tissue and moderate periglandular fibrosis. In conclusion, immunohistochemical analysis demonstrated an association between interleukins and inflammatory cells with endometrial lesions. In addition, this research may be useful in the future to evaluate the progress of the inflammatory process, contributing to the adequate optimization of the reproductive management of the mares.
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Publication Date: 2019-03-21 PubMed ID: 30900135DOI: 10.1007/s11033-018-04579-5Google Scholar: Lookup
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Summary

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The research studied the relationship between the expression of interleukins 6 and 10 and the presence of inflammatory cells in the equine endometrium. Findings confirm a correlation between these factors and endometrial lesions, potentially aiding in future research in understanding reproductive issues in mares.

Introduction and Purpose of the Study

  • The study aimed at investigating the role of interleukin 6 (IL-6) and 10 (IL-10) in endometritis, an inflammatory condition in mares that impacts fertility. Understanding these connections may provide insights to optimize reproductive management of mares.

Methodology

  • The research team used twelve crossbred cyclic mares for endometrial biopsy collection, specifically during the diestrus phase, which is 6 ± 1 day after ovulation.
  • Two key evaluation methods were used: histopathological analysis, to study tissue changes on a microscopic level, and immunohistochemical analysis to determine the presence and measurement of IL-6 and IL-10, as well as inflammatory cells.

Findings

  • Samples analyzed were classified based on the type and intensity of inflammation, alterations in endometrial fibrosis, and by biopsy categories (I, IIA, IIB, III).
  • The study reports an association between a low score of IL-6 in the endometrial glandular area and moderate fibrotic nets. This information suggests that a decreased presence of IL-6 might correlate with an increase in fibrotic changes in mares suffering from endometritis.
  • A high score for IL-10 in the sub-epithelial connective tissue was associated with moderate periglandular fibrosis. This indicates that high secretion of IL-10 may further promote fibrotic changes.

Conclusion

  • This study showed a clear association between the interleukins IL-6 and IL-10 and inflammatory cells with endometrial lesions in mares.
  • Finding such a connection enhances the understanding of the inflammatory processes that result in endometritis and may also assist in the effective management of mare reproduction.

Cite This Article

APA
de Holanda AGB, da Silva Leite J, Consalter A, da Silva KVGC, Dos Santos Batista BP, Fonseca ABM, Brandão FZ, Ferreira AMR. (2019). Expression of interleukins 6 and 10 and population of inflammatory cells in the equine endometrium: diagnostic implications. Mol Biol Rep, 46(2), 2485-2491. https://doi.org/10.1007/s11033-018-04579-5

Publication

Molecular biology reports
ISSN: 1573-4978
NlmUniqueID: 0403234
Country: Netherlands
Language: English
Volume: 46
Issue: 2
Pages: 2485-2491

Researcher Affiliations

de Holanda, Aline Gomes Buarque
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil. alinearaujo@id.uff.br.
da Silva Leite, Juliana
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil.
  • Laboratório de Anatomia Patológica Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Consalter, Angélica
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil.
da Silva, Kassia Valéria Gomes Coelho
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil.
  • Laboratório de Anatomia Patológica Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Dos Santos Batista, Barbara Paula
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil.
Fonseca, Ana Beatriz Monteiro
  • Instituto de Matemática e Estatística, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Brandão, Felipe Zandonadi
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil.
  • Setor de Reprodução Animal, Universidade Federal Fluminense, Niterói, Brazil.
Ferreira, Ana Maria Reis
  • Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Niterói, RJ, Brazil. ana_ferreira@id.uff.br.
  • Laboratório de Anatomia Patológica Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil. ana_ferreira@id.uff.br.

MeSH Terms

  • Animals
  • Biopsy / methods
  • Endometritis / diagnosis
  • Endometritis / immunology
  • Endometritis / metabolism
  • Endometrium / immunology
  • Endometrium / metabolism
  • Female
  • Horses / genetics
  • Horses / immunology
  • Inflammation / genetics
  • Inflammation / immunology
  • Interleukin-10 / analysis
  • Interleukin-10 / genetics
  • Interleukin-6 / analysis
  • Interleukin-6 / genetics
  • Uterus / pathology

References

This article includes 26 references
  1. Mallat Z, Besnard S, Duriez M, Deleuze V, Emmanuel F, Bureau MF, Soubrier F, Esposito B, Duez H, Fievet C, Staels B, Duverger N, Scherman D, Tedgui A. Protective role of interleukin-10 in atherosclerosis.. Circ Res 1999 Oct 15;85(8):e17-24.
    pubmed: 10521249doi: 10.1161/01.res.85.8.e17google scholar: lookup
  2. LeBlanc MM, Neuwirth L, Jones L, Cage C, Mauragis D. Differences in uterine position of reproductively normal mares and those with delayed uterine clearance detected by scintigraphy.. Theriogenology 1998 Jul 1;50(1):49-54.
    pubmed: 10734473doi: 10.1016/s0093-691x(98)00112-5google scholar: lookup
  3. Rigby SL, Barhoumi R, Burghardt RC, Colleran P, Thompson JA, Varner DD, Blanchard TL, Brinsko SP, Taylor T, Wilkerson MK, Delp MD. Mares with delayed uterine clearance have an intrinsic defect in myometrial function.. Biol Reprod 2001 Sep;65(3):740-7.
    pubmed: 11514336doi: 10.1095/biolreprod65.3.740google scholar: lookup
  4. Denys A, Udalova IA, Smith C, Williams LM, Ciesielski CJ, Campbell J, Andrews C, Kwaitkowski D, Foxwell BM. Evidence for a dual mechanism for IL-10 suppression of TNF-alpha production that does not involve inhibition of p38 mitogen-activated protein kinase or NF-kappa B in primary human macrophages.. J Immunol 2002 May 15;168(10):4837-45.
    pubmed: 11994432doi: 10.4049/jimmunol.168.10.4837google scholar: lookup
  5. Bolger AP, Sharma R, von Haehling S, Doehner W, Oliver B, Rauchhaus M, Coats AJ, Adcock IM, Anker SD. Effect of interleukin-10 on the production of tumor necrosis factor-alpha by peripheral blood mononuclear cells from patients with chronic heart failure.. Am J Cardiol 2002 Aug 15;90(4):384-9.
    pubmed: 12161227doi: 10.1016/s0002-9149(02)02494-3google scholar: lookup
  6. Bracher V, Allen WR. Videoendoscopic evaluation of the mare's uterus: I. Findings in normal fertile mares.. Equine Vet J 1992 Jul;24(4):274-8.
    pubmed: 1499534doi: 10.1111/j.2042-3306.1992.tb02834.xgoogle scholar: lookup
  7. Hemberg E, Lundeheim N, Einarsson S. Retrospective study on vulvar conformation in relation to endometrial cytology and fertility in thoroughbred mares.. J Vet Med A Physiol Pathol Clin Med 2005 Nov;52(9):474-7.
    pubmed: 16268960doi: 10.1111/j.1439-0442.2005.00760.xgoogle scholar: lookup
  8. Evans MJ, Hamer JM, Gason LM, Graham CS, Asbury AC, Irvine CH. Clearance of bacteria and non-antigenic markers following intra-uterine inoculation into maiden mares: Effect of steroid hormone environment.. Theriogenology 1986 Jul;26(1):37-50.
    pubmed: 16726168doi: 10.1016/0093-691x(86)90110-xgoogle scholar: lookup
  9. Carnevale EM, Ginther OJ. Relationships of age to uterine function and reproductive efficiency in mares.. Theriogenology 1992 May;37(5):1101-15.
    pubmed: 16727108doi: 10.1016/0093-691x(92)90108-4google scholar: lookup
  10. Kaur K, Sharma AK, Dhingra S, Singal PK. Interplay of TNF-alpha and IL-10 in regulating oxidative stress in isolated adult cardiac myocytes.. J Mol Cell Cardiol 2006 Dec;41(6):1023-30.
    pubmed: 17045606doi: 10.1016/j.yjmcc.2006.08.005google scholar: lookup
  11. Schlafer DH. Equine endometrial biopsy: enhancement of clinical value by more extensive histopathology and application of new diagnostic techniques?. Theriogenology 2007 Aug;68(3):413-22.
    pubmed: 17560641doi: 10.1016/j.theriogenology.2007.04.040google scholar: lookup
  12. Troedsson MH, Liu IK. Uterine clearance of non-antigenic markers (51Cr) in response to a bacterial challenge in mares potentially susceptible and resistant to chronic uterine infections.. J Reprod Fertil Suppl 1991;44:283-8.
    pubmed: 1795272
  13. Kaiko GE, Horvat JC, Beagley KW, Hansbro PM. Immunological decision-making: how does the immune system decide to mount a helper T-cell response?. Immunology 2008 Mar;123(3):326-38.
    pubmed: 17983439doi: 10.1111/j.1365-2567.2007.02719.xgoogle scholar: lookup
  14. Painter JT, Clayton NP, Herbert RA. Useful immunohistochemical markers of tumor differentiation.. Toxicol Pathol 2010 Jan;38(1):131-41.
    pubmed: 20028992doi: 10.1177/0192623309356449google scholar: lookup
  15. Jiwakanon J, Persson E, Berg M, Dalin AM. Influence of seminal plasma, spermatozoa and semen extender on cytokine expression in the porcine endometrium after insemination.. Anim Reprod Sci 2011 Feb;123(3-4):210-20.
    pubmed: 21196090doi: 10.1016/j.anireprosci.2010.11.016google scholar: lookup
  16. Snider TA, Sepoy C, Holyoak GR. Equine endometrial biopsy reviewed: observation, interpretation, and application of histopathologic data.. Theriogenology 2011 Jun;75(9):1567-81.
    pubmed: 21356552doi: 10.1016/j.theriogenology.2010.12.013google scholar: lookup
  17. Woodward EM, Christoffersen M, Campos J, Squires EL, Troedsson MH. Susceptibility to persistent breeding-induced endometritis in the mare: relationship to endometrial biopsy score and age, and variations between seasons.. Theriogenology 2012 Aug;78(3):495-501.
    pubmed: 22494681doi: 10.1016/j.theriogenology.2012.02.028google scholar: lookup
  18. Szóstek AZ, Lukasik K, Galvão AM, Ferreira-Dias GM, Skarzynski DJ. Impairment of the interleukin system in equine endometrium during the course of endometrosis.. Biol Reprod 2013 Oct;89(4):79.
    pubmed: 23946535doi: 10.1095/biolreprod.113.109447google scholar: lookup
  19. Klein V, Müller K, Schoon HA, Reilas T, Rivera del Alamo MM, Katila T. Effects of Intrauterine Devices in Mares: A Histomorphological and Immunohistochemical Evaluation of the Endometrium.. Reprod Domest Anim 2016 Feb;51(1):98-104.
    pubmed: 26661561doi: 10.1111/rda.12651google scholar: lookup
  20. Christoffersen M, Troedsson M. Inflammation and fertility in the mare.. Reprod Domest Anim 2017 Aug;52 Suppl 3:14-20.
    pubmed: 28815848doi: 10.1111/rda.13013google scholar: lookup
  21. Rua MAS, Quirino CR, Ribeiro RB, Carvalho ECQ, Bernadino MLA, Bartholazzi Junior A, Cipagalta LF, Barreto MAP. Diagnostic methods to detect uterus illnesses in mares.. Theriogenology 2018 Jul 1;114:285-292.
    pubmed: 29665574doi: 10.1016/j.theriogenology.2018.03.042google scholar: lookup
  22. Bain AM. The rôle of infection in infertility in the thoroughbred mare.. Vet Rec 1966 Jan 29;78(5):168-73.
    pubmed: 5948366doi: 10.1136/vr.78.5.168google scholar: lookup
  23. Kenney RM. Cyclic and pathologic changes of the mare endometrium as detected by biopsy, with a note on early embryonic death.. J Am Vet Med Assoc 1978 Feb 1;172(3):241-62.
    pubmed: 621166
  24. Cassatella MA, Meda L, Gasperini S, Calzetti F, Bonora S. Interleukin 10 (IL-10) upregulates IL-1 receptor antagonist production from lipopolysaccharide-stimulated human polymorphonuclear leukocytes by delaying mRNA degradation.. J Exp Med 1994 May 1;179(5):1695-9.
    pubmed: 8163946doi: 10.1084/jem.179.5.1695google scholar: lookup
  25. Troedsson MH, deMoraes MJ, Liu IK. Correlations between histologic endometrial lesions in mares and clinical response to intrauterine exposure with Streptococcus zooepidemicus.. Am J Vet Res 1993 Apr;54(4):570-2.
    pubmed: 8484576
  26. . Proceedings of the John P. Hughes International Workshop on Equine Endometritis. Davis, California, August 1992.. Equine Vet J 1993 May;25(3):184-93.
    pubmed: 8508744doi: 10.1111/j.2042-3306.1993.tb02940.xgoogle scholar: lookup

Citations

This article has been cited 5 times.
  1. Zhang W, Yuan Y, Huang G, Xiao J. Potential Molecular Mechanism of Guishen Huoxue Decoction against Intrauterine Adhesion Based on Network Pharmacology. Evid Based Complement Alternat Med 2022;2022:4049147.
    doi: 10.1155/2022/4049147pubmed: 36193142google scholar: lookup
  2. Katila T, Ferreira-Dias G. Evolution of the Concepts of Endometrosis, Post Breeding Endometritis, and Susceptibility of Mares. Animals (Basel) 2022 Mar 19;12(6).
    doi: 10.3390/ani12060779pubmed: 35327176google scholar: lookup
  3. Rivera Del Alamo MM, Reilas T, Lukasik K, Galvão AM, Yeste M, Katila T. Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation. Animals (Basel) 2021 Dec 8;11(12).
    doi: 10.3390/ani11123493pubmed: 34944269google scholar: lookup
  4. Yi J, Zhang P, Chen L, Chu S, Li L. Diagnostic value of CD20 combined with CD138 positive expression in patients with chronic endometritis. Am J Clin Exp Immunol 2025;14(3):145-156.
    doi: 10.62347/LIHI3143pubmed: 40689313google scholar: lookup
  5. Wei Y, Su H, Li J, Zheng W, Li L, Zhou M, Sun Y. Exploration into the Mechanism of Yiyi Baijiang Decoction Attenuating Chronic Pelvic Inflammation Based on Network Pharmacology and Experimental Verification. Biochem Genet 2025 Aug;63(4):2879-2892.
    doi: 10.1007/s10528-024-10843-0pubmed: 38856955google scholar: lookup
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