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Home / Equine Research Bank / Animals (Basel) / Expression of Oxytocin/Neurophysin I and Oxytocinase in the ...
Animals : an open access journal from MDPI2022; 12(7); 799; doi: 10.3390/ani12070799

Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation.

Abstract: Leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase) is an enzyme that metabolizes oxytocin in serum and tissues. The presence of oxytocin/neurophysin I (OXT), oxytocin and LNPEP and their relationship to other genes is unknown in the equine conceptus. Our objective was to characterize gene expression of LNPEP and OXT on D8, 10, 12, 14, 15, 16 and 21 conceptuses in relationship to other genes. Immunohistochemistry, western blot and liquid chromatography with tandem mass spectrometry (LC-MS/MS) were used for identification of oxytocin and LNPEP in D15, 16 and 18 conceptuses. LNPEP was increased at D15 compared to D10, was immunolocalized in the equine trophectoderm and endoderm, and protein was confirmed by LC-MS/MS. Maximal abundance of OXT was at D21, and lowest on D12 and D14, but no protein was identified. OXTR abundance was highest on D14 and D21. LNPEP was correlated with PTGFR and PTGES on D12 and D14-D15, and high expression of PTGES, PTGS2 was found on D14, D15 and D21; PTGFR was found on D8 and D12-21. LNPEP may have a role in prostaglandin regulation and conceptus fixation by decreasing the availability of oxytocin. Further investigation on the role embryonic LNPEP during pregnancy is warranted.
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Publication Date: 2022-03-22 PubMed ID: 35405789PubMed Central: PMC8996865DOI: 10.3390/ani12070799Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the expression of specific genes related to the hormone Oxytocin in equine (horse) embryos from day 8 to day 21 post-ovulation. The study identifies potential links between these genes and important reproductive events in horses, suggesting the need for further research.

Understanding the Research

  • The study aims to understand the activity of two key genes – Oxytocin/neurophysin I (OXT) and Leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase) – in the developing horse embryos (conceptus) from day 8 to day 21 post-ovulation. Specifically, the research characterizes the gene expression of LNPEP and OXT on these specific days and analyses their relationships to other genes.
  • OXT and LNPEP are important because they are respectively related to the production and metabolism of oxytocin, a hormone recognized for its role in childbirth and breastfeeding in humans, but possibly significant in equine reproduction too.
  • The research uses multiple techniques – immunohistochemistry, western blot, and liquid chromatography with tandem mass spectrometry (LC-MS/MS) – for the identification of oxytocin and LNPEP.

Key Findings

  • LNPEP was increasingly present on day 15 (D15) compared to day 10 (D10), localizing in particular parts of the conceptus (trophectoderm and endoderm). Researchers used LC-MS/MS to confirm this protein’s presence.
  • OXT abundance was highest on day 21 (D21) and lowest on days 12 (D12) and 14 (D14). However, the researchers found no identifiable protein for this particular gene.
  • The researchers also found strong correlations between LNPEP and other reproductive genes, particularly PTGFR and PTGES. This correlation suggests that LNPEP may have a role in the regulation of another hormone – prostaglandin, which is known to play a key role in various reproductive functions.

Implications and Future Research

  • The research provides some initial insights into the relationships between different genes within the horse embryo, particularly concerning oxytocin metabolism.
  • The observed connections suggest that the LNPEP gene could decrease the availability of oxytocin, which may affect the process of the embryo attaching to the uterus (conceptus fixation) – a crucial stage in pregnancy.
  • Due to these findings, further investigation into the role that the embryonic LNPEP gene plays in pregnancy could lead to new insights into equine reproduction, potentially informing improved equine breeding and care practices.

Cite This Article

APA
Diel de Amorim M, Klein C, Foster R, Dong L, Lopez-Rodriguez MF, Card C. (2022). Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation. Animals (Basel), 12(7), 799. https://doi.org/10.3390/ani12070799

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 7
PII: 799

Researcher Affiliations

Diel de Amorim, Mariana
  • Department of Clinical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY 14853, USA.
Klein, Claudia
  • Department of Veterinary Clinical and Diagnostic Science, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
Foster, Robert
  • Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E, Guelph, ON N1G 2W1, Canada.
Dong, Lynn
  • Immunopathology Research and Development Laboratory, Department of Biomedical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY 14853, USA.
Lopez-Rodriguez, Maria Fernanda
  • Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK S7N 5B4, Canada.
Card, Claire
  • Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK S7N 5B4, Canada.

Grant Funding

  • G00016114 / University of Saskatchewan

Conflict of Interest Statement

None of the authors have any conflict of interest to declare.

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Citations

This article has been cited 1 times.
  1. Diel de Amorim M, Dong L, Byron M, Foster RA, Klein C, Saleh M, Saleh T, Card C. Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare.. Sci Rep 2023 Mar 21;13(1):4616.
    doi: 10.1038/s41598-023-31540-9pubmed: 36944665google scholar: lookup
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