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Home / Equine Research Bank / Vet Sci / Faecal Proteomics and Functional Analysis of Equine Melanocy...
Veterinary sciences2022; 9(2); 94; doi: 10.3390/vetsci9020094

Faecal Proteomics and Functional Analysis of Equine Melanocytic Neoplasm in Grey Horses.

Abstract: Equine melanocytic neoplasm (EMN) is a common disease in older grey horses. The purpose of this study was to examine the potential proteins throughout EMN stages from faecal proteomic outlining using functional analysis. Faecal samples were collected from the rectum of 25 grey horses divided into three groups; normal group without EMN ( = 10), mild EMN ( = 6) and severe EMN ( = 9). Based on the results, 5910 annotated proteins out of 8509 total proteins were assessed from proteomic profiling. We observed differentially expressed proteins (DEPs) between the normal group and the EMN group, and 109 significant proteins were obtained, of which 28 and 81 were involved in metabolic and non-metabolic functions, respectively. We found 10 proteins that play a key role in lipid metabolism, affecting the tumour microenvironment and, consequently, melanoma progression. Interestingly, FOSL1 (FOS like 1, AP-1 transcription factor subunit) was considered as a potential highly expressed protein in a mild EMN group involved in melanocytes cell and related melanoma. Diacylglycerol kinase (DGKB), TGc domain-containing protein (Tgm2), structural maintenance of chromosomes 4 (SMC4) and mastermind-like transcriptional coactivator 2 (MAML2) were related to lipid metabolism, facilitating melanoma development in the severe-EMN group. In conclusion, these potential proteins can be used as candidate biomarkers for the monitoring of early EMN, the development of EMN, further prevention and treatment.
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Publication Date: 2022-02-21 PubMed ID: 35202347PubMed Central: PMC8875177DOI: 10.3390/vetsci9020094Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examined proteins related to equine melanocytic neoplasm (EMN), a common disease in grey horses, by analyzing faecal samples. Researchers found differentially expressed proteins between the healthy horses and those with EMN, some of which could serve as potential early indicators or treatment targets for the disease.

Methodology

  • The researchers gathered faecal samples from the rectum of 25 grey horses that were divided into three groups: horses without EMN, horses with mild EMN, and horses with severe EMN.
  • They analyzed these samples using proteomic profiling to identify and assess proteins.

Findings

  • The scientists were able to identify and assess 5910 annotated proteins out of a total of 8509.
  • They found that there were differentially expressed proteins (DEPs) between the normal group and the EMN group, with 109 significant proteins identified.
  • It was observed that, of these proteins, 28 were involved in metabolic functions and 81 in non-metabolic functions.
  • The study also revealed that 10 of these proteins play a crucial role in lipid metabolism, which impacts the tumor environment and hence, the progression of melanoma.

Significant Proteins

  • A protein called FOSL1, which is involved in cells that produce melanin and related melanoma, was found to be highly expressed in the group with mild EMN.
  • Few other proteins identified like Diacylglycerol kinase (DGKB), TGc domain-containing protein (Tgm2), structural maintenance of chromosomes 4 (SMC4), and mastermind-like transcriptional coactivator 2 (MAML2) were found to be related to lipid metabolism. They were seen to facilitate the development of melanoma in the group with severe EMN.

Conclusion

  • According to the researchers, these proteins can potentially serve as candidate biomarkers for early detection and monitoring of EMN, as well as for the development of prevention and treatment strategies.

Cite This Article

APA
Tesena P, Kingkaw A, Phaonakrop N, Roytrakul S, Limudomporn P, Vongsangnak W, Kovitvadhi A. (2022). Faecal Proteomics and Functional Analysis of Equine Melanocytic Neoplasm in Grey Horses. Vet Sci, 9(2), 94. https://doi.org/10.3390/vetsci9020094

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 9
Issue: 2
PII: 94

Researcher Affiliations

Tesena, Parichart
  • Graduate Student in Animal Health and Biomedical Science Program, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Puttamonthon, Nakhon Pathom 73170, Thailand.
Kingkaw, Amornthep
  • Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Phaonakrop, Narumon
  • Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand.
Roytrakul, Sittiruk
  • Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand.
Limudomporn, Paviga
  • Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Vongsangnak, Wanwipa
  • Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Omics Center for Agriculture, Bioresources, Food, and Health, Kasetsart University (OmiKU), Bangkok 10900, Thailand.
Kovitvadhi, Attawit
  • Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

Grant Funding

  • P1950420 / Functional ingredient and Food innovation program, National Science and Technology Develop-ment Agency
  • 2565 / Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

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