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BMC veterinary research2015; 11; 63; doi: 10.1186/s12917-015-0361-5

Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.

Abstract: Recent studies have demonstrated numerous biological properties of mesenchymal stem cells and their potential application in treating complex diseases or injuries to tissues that have difficulty regenerating, such as those affecting the central and peripheral nervous system. Thus, therapies that use mesenchymal stem cells are promising because of their high capacity for self-regeneration, their low immunogenicity, and their paracrine, anti-inflammatory, immunomodulatory, anti-apoptotic and neuroprotective effects. In this context, the purpose of this study was to evaluate the feasibility and safety of intrathecal transplantation of bone marrow-derived mesenchymal stem cells in horses, for future application in the treatment of neurological diseases. Results: During the neurological evaluations, no clinical signs were observed that were related to brain and/or spinal cord injury of the animals from the control group or the treated group. The hematological and cerebrospinal fluid results from day 1 and day 6 showed no significant differences (P > 0.05) between the treated group and the control group. Additionally, analysis of the expression of matrix metalloproteinase (MMP) -2 and -9 in the cerebrospinal fluid revealed only the presence of pro-MMP-2 (latent), with no significant difference (P > 0.05) between the studied groups. Conclusions: The results of the present study support the hypothesis of the feasibility and safety of intrathecal transplantation of autologous bone marrow-derived mesenchymal stem cells, indicating that it is a promising pathway for cell delivery for the treatment of neurological disorders in horses.
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Publication Date: 2015-03-15 PubMed ID: 25879519PubMed Central: PMC4369105DOI: 10.1186/s12917-015-0361-5Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research evaluates the possibility and safety of using a horse’s own bone marrow mesenchymal stem cells and transplanting them intrathecally (into the spinal canal or subarachnoid space) for the treatment of neurological diseases. The study concludes that this method of transplantation is both possible and safe, and offers promise for treating neurological disorders in horses.

Objective of the Research

  • The primary aim of this study was to assess the feasibility and safety of introducing autologous (originating from the same individual) bone marrow mesenchymal stem cells intrathecally in horses.
  • The researchers aim to explore its potential application in treating complex neurological diseases in the future.

Background of the Research

  • Past research has indicated the multiple biological properties of mesenchymal stem cells, and their potential in treating diseases or injuries to areas where tissue regeneration is difficult, including the central and peripheral nervous system.
  • These stem cells are compelling in therapeutic use due to their high self-regeneration abilities, their limited immunogenicity (ability to provoke an immune response), and their array of effects such as paracrine, anti-inflammatory, immunomodulatory, anti-apoptotic, and neuroprotective behaviors.

Research Methodology and Results

  • A cohort of horses underwent intrathecal transplantation of mesenchymal stem cells and were closely monitored to evaluate neurological indications and any signs related to brain or spinal cord injury.
  • These observations were compared to a control group of untreated horses.
  • The hematological and cerebrospinal fluid results collected on day 1 and day 6 after the procedure showed no significant variations between the untreated and treated group of horses.
  • The expression of matrix metalloproteinase (MMP)-2 and -9, a group of enzymes important in tissue remodeling, in the cerebrospinal fluid was also analyzed. Only the latent form of MMP-2 was found, with no significant variations between the groups.

Research Conclusion

  • The research concludes that the feasibility and safety of transplanting autologous bone marrow-derived mesenchymal stem cells intrathecally in horses is confirmed.
  • Indicating it as a promising methodology for cell delivery in the treatment of neurological disorders in horses.

Cite This Article

APA
Maia L, da Cruz Landim-Alvarenga F, Taffarel MO, de Moraes CN, Machado GF, Melo GD, Amorim RM. (2015). Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses. BMC Vet Res, 11, 63. https://doi.org/10.1186/s12917-015-0361-5

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 11
Pages: 63
PII: 63

Researcher Affiliations

Maia, Leandro
  • Department of Animal Reproduction, São Paulo State University, District of Rubião Júnior, n/n, CEP: 18618970, Botucatu, São Paulo, Brazil. leandromvet@hotmail.com.
da Cruz Landim-Alvarenga, Fernanda
  • Department of Animal Reproduction, São Paulo State University, District of Rubião Júnior, n/n, CEP: 18618970, Botucatu, São Paulo, Brazil. fernanda@fmvz.unesp.br.
Taffarel, Marilda Onghero
  • Department of Veterinary Medicine, Maringá State University, Av. Colombo, 5.790, CEP: 87020-900, Maringá, Paraná, Brazil. mtafarel@yahoo.com.br.
de Moraes, Carolina Nogueira
  • Department of Animal Reproduction, São Paulo State University, District of Rubião Júnior, n/n, CEP: 18618970, Botucatu, São Paulo, Brazil. carolnmoraes@hotmail.com.
Machado, Gisele Fabrino
  • Department of Clinic, Surgery and Animal Reproduction, São Paulo State University, Clóvis Pestano, 793, CEP: 16050-680, Araçatuba, São Paulo, Brazil. giselem@fmva.unesp.br.
Melo, Guilherme Dias
  • Department of Clinic, Surgery and Animal Reproduction, São Paulo State University, Clóvis Pestano, 793, CEP: 16050-680, Araçatuba, São Paulo, Brazil. di.melo@uol.com.br.
Amorim, Rogério Martins
  • Department of Veterinary Clinics, São Paulo State University, District of Rubião Júnior, n/n, CEP: 18618970, Botucatu, São Paulo, Brazil. rmamorim@fmvz.unesp.br.

MeSH Terms

  • Animals
  • Bone Marrow Transplantation / adverse effects
  • Bone Marrow Transplantation / methods
  • Bone Marrow Transplantation / veterinary
  • Feasibility Studies
  • Female
  • Horse Diseases / surgery
  • Horses
  • Injections, Spinal / veterinary
  • Male
  • Mesenchymal Stem Cell Transplantation / adverse effects
  • Mesenchymal Stem Cell Transplantation / methods
  • Mesenchymal Stem Cell Transplantation / veterinary
  • Nervous System Diseases / surgery
  • Nervous System Diseases / veterinary

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Citations

This article has been cited 6 times.
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