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Journal of nutritional science2014; 3; e10; doi: 10.1017/jns.2014.8

Fibre digestibility, abundance of faecal bacteria and plasma acetate concentrations in overweight adult mares.

Abstract: The purpose of the present study was to compare digestibility of grass hay, faecal and plasma volatile fatty acid (VFA) concentrations, and faecal bacterial abundance in overweight and moderate-condition mares. Five overweight adult mixed-breed mares and five adult mixed-breed mares in moderate condition were housed individually and limit-fed orchard grass (Dactylis glomerata) hay at 20 g/kg body weight (as fed) daily for 14 d. Forage DM and fibre digestibility were determined using AOAC methods; digestible energy was measured using bomb calorimetry; plasma and faecal VFA concentrations were determined by use of GC and MS; faecal Firmicutes, Bacteroidetes, Fibrobacter succinogenes, Ruminococcus flavefaciens and total bacteria abundance was determined by quantitative real-time PCR using previously designed phylum-specific 16S ribosomal RNA gene primers. No differences in hay digestibility, faecal VFA concentrations or faecal bacterial abundance were detected between overweight and moderate-condition mares. Mean plasma acetate concentrations were higher (P = 0·03) in overweight (1·55 (range 1·43-1·65) mmol/l) v. moderate-condition (1·39 (range 1·22-1·47) mmol/l) mares. We conclude that the higher plasma acetate in overweight mares should be further investigated as a potential link between gut microbes and obesity in horses.
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Publication Date: 2014-05-07 PubMed ID: 25191602PubMed Central: PMC4153333DOI: 10.1017/jns.2014.8Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the differences in dietary fibre digestion, volatile fatty acid (VFA) concentration, and bacteria presence in the faeces of overweight and moderate-weight mares, finding a higher concentration of plasma acetate in overweight mares that could potentially link gut microbes to obesity in horses.

Methodology

  • The study involved a comparison between five overweight mixed-breed adult mares and five moderate-condition mares of the same breed, keeping them individually limited to a daily intake of orchard grass hay for a period of 14 days.
  • The study utilized several internationally approved methods to conduct the research such as determining forage dry matter and fibre digestibility using AOAC methods while digestible energy was measured using bomb calorimetry.
  • For examining the fat concentrations in plasma and faeces VFA, a combination of gas chromatography (GC) and mass spectroscopy (MS) was used.
  • To estimate faecal bacterial presence, quantitative real-time polymerase chain reaction (qPCR) was conducted using phylum-specific 16S ribosomal RNA gene primers. The range of bacteria examined included Firmicutes, Bacteroidetes, Fibrobacter succinogenes, and Ruminococcus flavefaciens.

Findings

  • The findings indicated no evident differences in the digestibility of hay, concentrations of VFA in faeces, or the abundance of bacterial in faeces between the overweight and moderate-condition mares.
  • The average concentration of plasma acetate was found to be higher in overweight mares as compared to those in moderate-condition. The statistical significance was established with a value of P=0.03, the range of plasma acetate concentration for overweight mares was 1.43-1.65 mmol/l, while for moderate condition mares, it was 1.22-1.47 mmol/l.

Conclusions

  • Based on the raised plasma acetate levels in overweight mares, the research concludes to delve further in this direction. A higher concentration of this particular volatile fatty acid in overweight mares indicated a potential connecting factor between obesity in horses and gut microbes.
  • This observation can open up a new area of investigation in the study of horse obesity, potentially leading towards the development of new dietary or medicinal approaches to manage the global issue of equine obesity.

Cite This Article

APA
Shepherd ML, Ponder MA, Burk AO, Milton SC, Swecker WS. (2014). Fibre digestibility, abundance of faecal bacteria and plasma acetate concentrations in overweight adult mares. J Nutr Sci, 3, e10. https://doi.org/10.1017/jns.2014.8

Publication

Journal of nutritional science
ISSN: 2048-6790
NlmUniqueID: 101590587
Country: England
Language: English
Volume: 3
Pages: e10
PII: e10

Researcher Affiliations

Shepherd, Megan L
  • Department of Large Animal Clinical Sciences , Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech , Duck Pond Drive (0442), Blacksburg, VA 24061 , USA.
Ponder, Monica A
  • Department of Food Science and Technology , Virginia Tech , Blacksburg, VA 24061 , USA.
Burk, Amy O
  • Department of Animal and Avian Sciences , University of Maryland , College Park, MD 20742 , USA.
Milton, Stewart C
  • Department of Large Animal Clinical Sciences , Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech , Duck Pond Drive (0442), Blacksburg, VA 24061 , USA.
Swecker, William S
  • Department of Large Animal Clinical Sciences , Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech , Duck Pond Drive (0442), Blacksburg, VA 24061 , USA.

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Citations

This article has been cited 7 times.
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