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Physiological reports2024; 12(21); e70110; doi: 10.14814/phy2.70110

Fibrolytic efficiency of the large intestine microbiota may benefit running speed in French trotters: A pilot study.

Abstract: This pilot study sought to explore the contribution of the large intestine microbiota to energy metabolism and exercise performance through its ability to degrade fibers into short-chain fatty acids (SCFAs). To investigate this, a correlational study was carried out on athlete horses under the same management conditions. Fecal microbiota diversity and composition, fibrolytic efficiency and SCFAs were analyzed. An incremental running test was carried out to estimate the maximal running speed (MRS) of the horses, and blood samples were taken to measure energy metabolism parameters. MRS was positively correlated with the efficiency of the fecal microbiota in degrading cellulose in vitro (r = 0.51; p = 0.02). The abundance of fibrolytic bacterial taxa was not associated with MRS, but functional inference analysis revealed a positive association between MRS and pathways potentially related to fibrolytic activity (r = 0.54; p = 0.07 and r = 0.56; p = 0.05 for butyrate metabolism and thiamine metabolism, respectively). In contrast, the metabolic pathway of starch degradation appeared negatively associated with MRS (r = -0.55; p = 0.06). The present findings suggest a potential contribution of the large intestine microbiota and dietary fibers digestion to exercise capacity in equine athletes.
© 2024 The Author(s). Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.
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Publication Date: 2024-11-13 PubMed ID: 39533164PubMed Central: PMC11557442DOI: 10.14814/phy2.70110Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the possible influence of large intestine bacteria in horses on energy metabolism and exercise performance by breaking down fibers into short-chain fatty acids (SCFAs). The study found a correlation between the run speed of athlete horses and the efficacy of their fecal microbes in breaking down cellulose.

Study participants and procedures

  • The study was performed on athlete horses who were kept under the same management conditions. This helped eliminate variables related to diet, training, and living conditions that may affect the gut microbiota or the horses’ exercise performance.
  • A series of tests were conducted to monitor the fecal microbiota, their ability to degrade fibers (fibrolytic efficiency), and the production of short-chain fatty acids (SCFAs).
  • The researchers also performed an incremental running test to estimate the horses’ maximal running speed (MRS) and took blood samples to measure parameters associated with energy metabolism.

Results and findings

  • There was a positive correlation observed between the maximal running speed of the horses and the efficiency of their fecal microbiota in breaking down cellulose. Essentially, the better the gut bacteria were at degrading fibers into SCFAs, the faster the horses could run.
  • Interestingly, the abundance of fibrolytic bacteria – those that break down fibers – did not correlate with maximal running speed. Instead, some metabolic pathways related to fibrolytic activity showed a positive correlation with running speed.
  • Notably, an association was found between maximal running speed and the metabolism pathways for butyrate and thiamine (vitamin B1). Butyrate is a type of SCFA that serves as a primary energy source for the cells lining the colon.
  • Contrarily, the metabolic pathway of starch degradation appeared negatively associated with maximal running speed, suggesting that a more starch-oriented metabolism may not be favorable for running performance.
  • In conclusion, the study suggests that gut microbiota’s efficiency in breaking down dietary fibers may influence exercise capacity in horses, possibly by affecting the horse’s energy metabolism.

Cite This Article

APA
Vasseur M, Lepers R, Langevin N, Julliand S, Grimm P. (2024). Fibrolytic efficiency of the large intestine microbiota may benefit running speed in French trotters: A pilot study. Physiol Rep, 12(21), e70110. https://doi.org/10.14814/phy2.70110

Publication

Physiological reports
ISSN: 2051-817X
NlmUniqueID: 101607800
Country: United States
Language: English
Volume: 12
Issue: 21
Pages: e70110
PII: e70110

Researcher Affiliations

Vasseur, Maximilien
  • Institut National de la Santé et de la Recherche Médicale, Inserm UMR 1093, Cognition Action et Plasticité Sensorimotrice (CAPS), Faculty of Sport Sciences, Université de Bourgogne, Dijon, France.
  • Lab to Field, Dijon, France.
Lepers, Romuald
  • Institut National de la Santé et de la Recherche Médicale, Inserm UMR 1093, Cognition Action et Plasticité Sensorimotrice (CAPS), Faculty of Sport Sciences, Université de Bourgogne, Dijon, France.
Langevin, Nicolas
  • Ecurie Hunter Valley, Belfonds, France.
Julliand, Samy
  • Lab to Field, Dijon, France.
Grimm, Pauline
  • Lab to Field, Dijon, France.

MeSH Terms

  • Animals
  • Running / physiology
  • Pilot Projects
  • Gastrointestinal Microbiome / physiology
  • Horses
  • Feces / microbiology
  • Male
  • Physical Conditioning, Animal
  • Fatty Acids, Volatile / metabolism
  • Dietary Fiber / metabolism
  • Dietary Fiber / administration & dosage
  • Intestine, Large / microbiology
  • Intestine, Large / metabolism
  • Energy Metabolism / physiology
  • Female

Grant Funding

  • 2021/1600 / Association Nationale de la Recherche et de la Technologie (ANRT)
  • 1 / CSRD VA

Conflict of Interest Statement

No conflicts of interest, financial or otherwise, are declared by the authors.

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Citations

This article has been cited 1 times.
  1. Réka F, Zsófia B, Ádám B, Péter F. The Gut-Muscle-Immune Axis in Motion: Mechanistic Synergies of SCFA Metabolism, Exercise, and Microbial Cross-Feeding. Nutrients 2025 Dec 2;17(23).
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