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Home / Equine Research Bank / Vet Immunol Immunopathol / Flow cytometric characterization of culture expanded multipo...
Veterinary immunology and immunopathology2011; 144(3-4); 499-506; doi: 10.1016/j.vetimm.2011.07.017

Flow cytometric characterization of culture expanded multipotent mesenchymal stromal cells (MSCs) from horse adipose tissue: towards the definition of minimal stemness criteria.

Abstract: In the last decades, multipotent mesenchymal progenitor cells have been isolated from many adult tissues of different species. The International Society for Cellular Therapy (ISCT) has recently established that multipotent mesenchymal stromal cells (MSCs) is the currently recommended designation. In this study, we used flow cytometry to evaluate the expression of several molecules related to stemness (CD90, CD44, CD73 and STRO-1) in undifferentiated, early-passaged MSCs isolated from adipose tissue of four donor horses (AdMSCs). The four populations unanimously expressed high levels of CD90 and CD44. On the contrary, they were unexpectedly negative to CD73. A small percentage of the cells, finally, showed the expression of STRO-1. This last result might be due to the existence of a small subpopulation of STRO-1+ cells or to a poor cross-reactivity of the antibody. A remarkable donor-to-donor consistency and reproducibility of these findings was demonstrated. The data presented herein support the idea that equine AdMSCs may be easily isolated and selected by adherence to tissue culture plastic and exhibit a surface profile characterized by some peculiar differences in comparison to those described in other species. Continued characterization of these cells will help to clarify several aspects of their biology and may ultimately enable the isolation of specific, purified subpopulations.
Copyright © 2011 Elsevier B.V. All rights reserved.
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Publication Date: 2011-07-26 PubMed ID: 21839521DOI: 10.1016/j.vetimm.2011.07.017Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper focuses on the use of flow cytometry in assessing molecules related to stem cells (CD90, CD44, CD73 and STRO-1) for the processes of differentiation and early passage in Multipotent Mesenchymal Stromal Cells (MSCs) obtained from the adipose tissue of horses.

Overview of the Study

  • The researchers isolate multipotent mesenchymal progenitor cells (a type of stem cell) from the adipose tissues of horses. The cells identified from these tissues were called multipotent mesenchymal stromal cells (MSCs).
  • The study uses flow cytometry, a technique that measures physical and chemical characteristics of a population of cells or particles, to evaluate the status of the cells. Four key molecules related to stemness – CD90, CD44, CD73 and STRO-1 – are assessed in the early-passaged, undifferentiated MSCs.

Main Findings

  • The findings reveal that all four populations consistently expressed high levels of CD90 and CD44, signaling they are indeed stem cells.
  • However, contrary to what might be expected, the cells were not CD73 positive. CD73 is a surface enzyme often found in MSCs, implying there might be certain differences in horse MSCs as compared to those from other species.
  • Only a small percentage of the cells displayed STRO-1 expression. This varies from the norm, where STRO-1 is commonly expressed in MSCs. The researchers suggest that this could either be due to a small subpopulation of STRO-1 positive cells or the possible poor reactive properties of the antibody used to detect STRO-1.

Significance and Future Implications

  • The findings signify that equine or horse MSCs can be easily isolated and selected based on their ability to adhere to tissue culture plastic. Moreover, the surface profile of these cells differs when compared to other species, hinting at species-specific properties.
  • The research contributes to the ongoing effort to define the minimum stemness criteria. By identifying the different states of molecules typically expressed in MSCs, the study paves the way for a deeper understanding of the biology of these cells.
  • An important implication for future research is the opportunity to identify and isolate specific, purified sub-populations of these cells, which might have therapeutic applications in cell localisation and tissue repair.

Cite This Article

APA
Pascucci L, Curina G, Mercati F, Marini C, Dall'Aglio C, Paternesi B, Ceccarelli P. (2011). Flow cytometric characterization of culture expanded multipotent mesenchymal stromal cells (MSCs) from horse adipose tissue: towards the definition of minimal stemness criteria. Vet Immunol Immunopathol, 144(3-4), 499-506. https://doi.org/10.1016/j.vetimm.2011.07.017

Publication

Veterinary immunology and immunopathology
ISSN: 1873-2534
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 144
Issue: 3-4
Pages: 499-506

Researcher Affiliations

Pascucci, L
  • Department of Biopathological Science and Animal and Food Hygiene, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy. luisa.pascucci@unipg.it
Curina, G
    Mercati, F
      Marini, C
        Dall'Aglio, C
          Paternesi, B
            Ceccarelli, P

              MeSH Terms

              • 5'-Nucleotidase / analysis
              • Adipose Tissue / cytology
              • Adipose Tissue / immunology
              • Adipose Tissue / physiology
              • Animals
              • Antigens, Surface / analysis
              • Cells, Cultured
              • Female
              • Flow Cytometry / veterinary
              • Horses
              • Hyaluronan Receptors / analysis
              • Male
              • Multipotent Stem Cells / chemistry
              • Multipotent Stem Cells / immunology
              • Multipotent Stem Cells / physiology
              • Thy-1 Antigens / analysis

              Citations

              This article has been cited 21 times.
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