Fluctuations in equine cutaneous pH and transepidermal water loss with time of day and ambient conditions.

This research investigates the natural changes in skin pH and transepidermal water loss (TEWL) levels in horses over various times of day and in different weather conditions. The study aims to deepen our understanding of horse skin health and function.

Objective of the Study

• The main objective of the study was to understand the natural fluctuations in the biophysical parameters of healthy horse skin. More specifically, the researchers wanted to explore how skin pH and TEWL levels in horses varied throughout the day and in different environmental conditions.

Methodology

• The research team collected skin pH and TEWL data from nine mares at three different times (6:00 AM, 12:00 PM, and 6:00 PM) every day for five days. The data was collected at five different anatomical locations – the nose, withers, girth area, mid-back, and base of the tail.
• The ambient temperature and humidity were also recorded each time the data was collected.
• Statistical analysis of the data was done using SAS On Demand. The tests that were carried out included repeated measures, ANOVA, and regression analysis.

Results and Findings

• The study found that the mean skin pH significantly differed by day and time, but it wasn’t affected by the anatomical location. Furthermore, there was a significant interaction between day and location in terms of skin pH.
• On the other hand, the TEWL measures significantly differed by day, time, and location. A significant interaction was also found among day, time, and location regarding TEWL.
• The research found no significant associations of skin pH with temperature and humidity. However, all TEWL measures across all locations were significantly correlated with temperature and humidity.

Implications of the Study

• The research concludes that both skin pH and TEWL measures in horses are influenced by environmental conditions. Therefore, environmental factors should be considered when studying the dermal characteristics of horses. The findings of this study can be used to improve our understanding of horse skin health and lead to more effective dermatological treatments for horses.