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Home / Equine Research Bank / J Anat / Gap junction protein expression and cellularity: comparison ...
Journal of anatomy2007; 211(3); 325-334; doi: 10.1111/j.1469-7580.2007.00781.x

Gap junction protein expression and cellularity: comparison of immature and adult equine digital tendons.

Abstract: Injury to the energy-storing superficial digital flexor tendon is common in equine athletes and is age-related. Tenocytes in the superficial digital flexor tendon of adult horses appear to have limited ability to respond adaptively to exercise or prevent the accumulation of strain-induced microdamage. It has been suggested that conditioning exercise should be introduced during the growth period, when tenocytes may be more responsive to increased quantities or intensities of mechanical strain. Tenocytes are linked into networks by gap junctions that allow coordination of synthetic activity and facilitate strain-induced collagen synthesis. We hypothesised that there are reductions in cellular expression of the gap junction proteins connexin (Cx) 43 and 32 during maturation and ageing of the superficial digital flexor tendon that do not occur in the non-injury-prone common digital extensor tendon. Cryosections from the superficial digital flexor tendon and common digital extensor tendon of 5 fetuses, 5 foals (1-6 months), 5 young adults (2-7 years) and 5 old horses (18-33 years) were immunofluorescently labelled and quantitative confocal laser microscopy was performed. Expression of Cx43 and Cx32 protein per tenocyte was significantly higher in the fetal group compared with all other age groups in both tendons. The density of tenocytes was found to be highest in immature tissue. Higher levels of cellularity and connexin protein expression in immature tendons are likely to relate to requirements for tissue remodelling and growth. However, if further studies demonstrate that this correlates with greater gap junctional communication efficiency and synthetic responsiveness to mechanical strain in immature compared with adult tendons, it could support the concept of early introduction of controlled exercise as a means of increasing resistance to later injury.
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Publication Date: 2007-09-13 PubMed ID: 17848160PubMed Central: PMC2375813DOI: 10.1111/j.1469-7580.2007.00781.xGoogle Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the role of gap junction proteins in the tendons of horses, and their change during maturation and ageing. Findings suggest that the proteins, connexin 43 and 32, are significantly more abundant in fetal tissues, implying that controlled exercise introduced early may help increase resistance to injury later in life.

Understanding the Research

The research was driven by the observation that equine athlete injuries are common and often linked to age. These injuries often involve the energy-storing superficial digital flexor tendon. Adult horse tenocytes – the cells found in tendons and ligaments – seem to have a limited ability to adapt and prevent strain-induced damage. Early conditioning exercise may increase their responsiveness.

  • The research tested the hypothesis that the decrease in connexin 43 and 32 gap junction proteins – which have a role in coordinating synthetic activity and strain-induced collagen synthesis among tenocytes – will not occur in an injury-resistant tendon (common digital extensor tendon) but would be seen in the superficial digital flexor tendon as it matures and ages.
  • The comparison of these proteins’ expression was done using tissue samples from the tendons of fetuses, foals (1-6 months old), young adult (2-7 years old), and old horses (18-33 years old). The study used immunofluorescent labeling and quantitative confocal laser microscopy for this purpose.

Key Findings

  • The research found that the expression of connexin 43 and 32 per tenocyte was significantly higher in the fetal stage than all the other age groups studied, regardless of tendon type.
  • The density of tenocytes in immature tissue was found to be the highest, indicating higher cellularity and protein expression at this stage for tissue remodelling and growth.
  • This leads to the possibility that increased gap junctional communication and synthetic responsiveness to mechanical strain at the immature stage compared to adult tendons could explain the reduced injury resistance in adults.

Significance and Future Implications

  • The study sheds light on the importance of gap junction proteins in maintaining the health of equine tendons.
  • The maturity-dependent decrease in these proteins can partially explain the higher susceptibility of adult horses to tendon injuries.
  • If further research supports these findings, it could validate the idea of introducing controlled exercise at an early stage of horse development, thereby improving their injury resistance later in life.

Cite This Article

APA
Stanley RL, Fleck RA, Becker DL, Goodship AE, Ralphs JR, Patterson-Kane JC. (2007). Gap junction protein expression and cellularity: comparison of immature and adult equine digital tendons. J Anat, 211(3), 325-334. https://doi.org/10.1111/j.1469-7580.2007.00781.x

Publication

Journal of anatomy
ISSN: 0021-8782
NlmUniqueID: 0137162
Country: England
Language: English
Volume: 211
Issue: 3
Pages: 325-334

Researcher Affiliations

Stanley, Rachael L
  • Department of Pathology and Infectious Diseases, Royal Veterinary College, Hatfield, Hertfordshire, UK. rstanley@rvc.ac.uk
Fleck, Roland A
    Becker, David L
      Goodship, Allen E
        Ralphs, Jim R
          Patterson-Kane, Janet C

            MeSH Terms

            • Aging / physiology
            • Animals
            • Cell Count
            • Connexin 43 / analysis
            • Connexins / analysis
            • Fluorescent Antibody Technique
            • Forelimb
            • Horses / physiology
            • Microscopy, Confocal
            • Physical Conditioning, Animal
            • Stress, Mechanical
            • Tendons / cytology
            • Tendons / growth & development
            • Tendons / metabolism

            Grant Funding

            • BB/D524883/1 / Biotechnology and Biological Sciences Research Council

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