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Frontiers in veterinary science2021; 8; 684203; doi: 10.3389/fvets.2021.684203

Gastrin and Nitric Oxide Production in Cultured Gastric Antral Mucosa Are Altered in Response to a Gastric Digest of a Dietary Supplement.

Abstract: organ culture can provide insight into isolated mucosal responses to particular environmental stimuli. The objective of the present study was to investigate the impact of a prolonged culturing time as well as the addition of acidic gastric fluid into the environment of cultured gastric antral tissue to evaluate how altering the commonly used neutral environment impacted tissue. Furthermore, we aimed to investigate the impact of G's Formula, a dietary supplement for horses, on the secretion of gastrin, interleukin1-beta (IL-1β), and nitric oxide (NO). These biomarkers are of interest due to their effects on gastric motility and mucosal activity. Gastric mucosal tissue explants from porcine stomachs were cultured in the presence of a simulated gastric fluid (BL, = 14), simulated gastric fluid containing the dietary supplement G's Formula (DF, = 12), or an equal volume of phosphate buffered saline (CO, = 14). At 48 and 60 h, 10 M carbachol was used to stimulate gastrin secretion. Cell viability was assessed at 72 h using calcein and ethidium-homodimer 1 staining. Media was analyzed for gastrin, IL-1β, and NO at 48, 60, and 72 h. There were no effects of treatment or carbachol stimulation on explant cell viability. Carbachol resulted in a significant increase in gastrin concentration in CO and DF treatments, but not in BL. NO was higher in CO than in BL, and NO increased in the CO and DF treatments but not in BL. In conclusion, the addition of carbachol and gastric digests to culture media did not impact cell viability. The use of an acidic gastric digest (BL) reduced the effect of cholinergic stimulation with carbachol at a concentration of 10 M and reduced NO secretion. The addition of the dietary supplement to the gastric digest (DF) appeared to mediate these effects within this model. Further research is required to evaluate the specific effects of this dietary supplement on direct markers of mucosal activity and the functional relevance of these results .
Copyright © 2021 MacNicol and Pearson.
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Publication Date: 2021-10-04 PubMed ID: 34671658PubMed Central: PMC8520902DOI: 10.3389/fvets.2021.684203Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper explores the impact of dietary supplement G’s formula and different environmental conditions on the secretion of various biomarkers like gastrin, interleukin1-beta (IL-1β), and nitric oxide (NO) in cultured gastric antral tissue. It indicates that an acidic gastric fluid may reduce the effect of a certain experimental stimulation called carbachol and NO secretion, with the dietary supplement possibly influencing these effects.

Understanding the Research Objectives

  • The paper aims at observing the behavior of gastric antral tissue in response to different environments in vitro during a long culturing time.
  • It wants to study the impact of changing the usual neutral environment to one containing acidic gastric fluid (similar to the environment of the stomach).
  • The paper focuses on exploring the influence of G’s Formula, a horse dietary supplement on the secretion of gastrin, IL-1β, and NO, which are biomarkers affecting gastric motility and mucosal activity.

The Experimental Procedure

  • An experiment was set up where gastric tissue explants (samples) from pig stomachs were cultured in three different substances – a simulated gastric fluid, a simulated gastric fluid containing G’s Formula, or a saline solution.
  • The experiment was designed for a total duration of 72 hours, and gastrin secretion was stimulated at the 48-hour and 60-hour marks using a chemical called carbachol.
  • After 72 hours, the viability of the cells was assessed using certain staining techniques. Samples were also analyzed for levels of gastrin, IL-1β, and NO at 48, 60, and 72 hours.

Discussion of the Results

  • The results of the experiment demonstrated that cell viability was not affected by either the treatment or the stimulation from carbachol.
  • Carbachol was found to significantly increase the concentration of gastrin in the samples treated with saline and the dietary supplement but not in the acidic environment.
  • NO levels were observed to be higher in the saline environment than in the acidic one. Increase in NO was observed in both the saline and supplement treatments but not in the acidic fluid.
  • These results demonstrate that the use of an acidic gastric fluid can potentially reduce the effect of cholinergic stimulation with carbachol and NO secretion.

Conclusions and Further Studies

  • The research concludes that adding carbachol and gastric digests to culture media didn’t impact cell viability, but the use of an acidic gastric fluid reduced the effect of carbachol on increasing gastrin concentration and also lowered NO secretion.
  • The dietary supplement seemed to mitigate these effects. However, the authors emphasize that more research into the impact of this dietary supplement on mucosal activity markers is needed to understand its functional relevance.

Cite This Article

APA
MacNicol JL, Pearson W. (2021). Gastrin and Nitric Oxide Production in Cultured Gastric Antral Mucosa Are Altered in Response to a Gastric Digest of a Dietary Supplement. Front Vet Sci, 8, 684203. https://doi.org/10.3389/fvets.2021.684203

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 8
Pages: 684203
PII: 684203

Researcher Affiliations

MacNicol, Jennifer L
  • Department of Animal Biosciences, Ontario Agricultural College, University of Guelph, Guelph, ON, Canada.
Pearson, Wendy
  • Department of Animal Biosciences, Ontario Agricultural College, University of Guelph, Guelph, ON, Canada.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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