Gene biomarkers in peripheral white blood cells of horses with experimentally induced osteoarthritis.
Abstract: To use microarray analysis to identify genes that are differentially expressed in horses with experimentally induced osteoarthritis. Methods: 24 horses. Methods: During arthroscopic surgery, a fragment was created in the distal aspect of the radiocarpal bone in 1 forelimb of each horse to induce osteoarthritis. At day 14 after osteoarthritis induction, horses began exercise on a treadmill. Blood and synovial fluid samples were collected before and after surgery. At day 70, horses were euthanized and tissues were harvested for RNA analysis. An equine-specific microarray was used to measure RNA expression in peripheral WBCs. These data were compared with mRNA expression (determined via PCR assay) in WBCs, cartilage, and synovium as well as 2 protein biomarkers of cartilage matrix turnover in serum and synovial fluid. Results: A metalloproteinase domain-like protein decysin-1 (ADAMDEC1), glucose-regulated protein (GRP) 94, hematopoietic cell signal transducer (HCST), Unc-93 homolog A (hUNC-93A), and ribonucleotide reductase M2 polypeptide (RRM2) were significantly differentially regulated in WBCs of horses with osteoarthritis, compared with values prior to induction of osteoarthritis. There was correlation between the gene expression profile in WBCs, cartilage, and synovium and the cartilage turnover proteins. Gene expression of ADAMDEC1, hUNC-93A, and RRM2 in WBCs were correlated when measured via microarray analysis and PCR assay. Conclusions: Expression of ADAMDEC1, GRP94, HCST, hUNC-93A, and RRM2 was differentially regulated in peripheral WBCs obtained from horses with experimentally induced osteoarthritis. Gene expression of ADAMDEC1, hUNC-93A, and RRM2 in peripheral WBCs has the potential for use as a diagnostic aid for osteoarthritis in horses.
Publication Date: 2012-12-29 PubMed ID: 23270355DOI: 10.2460/ajvr.74.1.115Google Scholar: Lookup
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- Journal Article
Summary
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The research study aims to identify genes differentially expressed in horses with experimentally induced osteoarthritis, which could potentially be used as diagnostic aids. Microarray analysis has been utilized to analyze genes in peripheral white blood cells, along with a comparison with mRNA expression in white blood cells, cartilage and synovial fluid.
Methodology
- The study involved a total of 24 horses. Osteoarthritis was experimentally induced in these horses by creating a fragment in the distal aspect of the radiocarpal bone in one forelimb during arthroscopic surgery. Post the induction of osteoarthritis, a 14-day exercise regiment on a treadmill was implemented.
- Samples of blood and synovial fluid were collected from the horses both before and after the surgical procedure, and at day 70 post surgery, all the horses were euthanized and tissues harvested for further RNA analysis.
- An equine-specific microarray was employed to measure the RNA expression in peripheral white blood cells (WBCs), and these results were juxtaposed with general mRNA expression in WBCs, cartilage, and synovial fluid.
- Additionally, the expressions of two protein biomarkers indicating cartilage matrix turnover in serum and synovial fluid were studied.
Results
- The results identified a metalloproteinase domain-like protein (ADAMDEC1), glucose-regulated protein 94 (GRP94), hematopoietic cell signal transducer (HCST), Unc-93 homolog A (hUNC-93A), and ribonucleotide reductase M2 polypeptide (RRM2) as Differential gene regulators in WBCs of horses with osteoarthritis versus pre-induction values.
- The study also discovered a correlation between the gene expression profile in WBCs, cartilage, and synovium with the cartilage turnover proteins. Moreover, a correlation was established between the gene expression of ADAMDEC1, hUNC-93A, and RRM2 in WBCs when measured via microarray analysis and PCR assay.
Conclusion
- The differential regulation of ADAMDEC1, GRP94, HCST, hUNC-93A, and RRM2 expression was observed in peripheral WBCs sourced from horses with experimentally induced osteoarthritis.
- The research concluded that the gene expression of ADAMDEC1, hUNC-93A, and RRM2 in peripheral WBCs can potentially be used as a diagnostic aid for identifying osteoarthritis in horses.
Cite This Article
APA
Kamm JL, Frisbie DD, McIlwraith CW, Orr KE.
(2012).
Gene biomarkers in peripheral white blood cells of horses with experimentally induced osteoarthritis.
Am J Vet Res, 74(1), 115-121.
https://doi.org/10.2460/ajvr.74.1.115 Publication
Researcher Affiliations
- Gail Holmes Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.
MeSH Terms
- Animals
- Biomarkers / blood
- Carpus, Animal / injuries
- Carpus, Animal / pathology
- Cartilage / metabolism
- Gene Expression Regulation
- Horse Diseases / blood
- Horses
- Lameness, Animal / blood
- Leukocytes / metabolism
- Osteoarthritis / blood
- Osteoarthritis / veterinary
- Protein Array Analysis / veterinary
- RNA, Messenger / metabolism
- Serum / metabolism
- Synovial Membrane / metabolism
- Transcriptome
Citations
This article has been cited 6 times.- Pan J, Purev C, Zhao H, Zhang Z, Wang F, Wendoule N, Qi G, Liu Y, Zhou H. Discovery of exercise-related genes and pathway analysis based on comparative genomes of Mongolian originated Abaga and Wushen horse.. Open Life Sci 2022;17(1):1269-1281.
- Korostyński M, Małek N, Piechota M, Starowicz K. Blood Transcriptional Signatures for Disease Progression in a Rat Model of Osteoarthritis.. Int J Genomics 2017;2017:1746426.
- O'Shea NR, Chew TS, Dunne J, Marnane R, Nedjat-Shokouhi B, Smith PJ, Bloom SL, Smith AM, Segal AW. Critical Role of the Disintegrin Metalloprotease ADAM-like Decysin-1 [ADAMDEC1] for Intestinal Immunity and Inflammation.. J Crohns Colitis 2016 Dec;10(12):1417-1427.
- Mendoza L, Piquemal D, Lejeune JP, Vander Heyden L, Noguier F, Bruno R, Sandersen C, Serteyn D. Age-dependent expression of osteochondrosis-related genes in equine leukocytes.. Vet Rec Open 2015;2(1):e000058.
- Malfait AM, Little CB. On the predictive utility of animal models of osteoarthritis.. Arthritis Res Ther 2015 Sep 14;17(1):225.
- Sánchez J, Bonet ML, Keijer J, van Schothorst EM, Mölller I, Chetrit C, Martinez-Puig D, Palou A. Blood cells transcriptomics as source of potential biomarkers of articular health improvement: effects of oral intake of a rooster combs extract rich in hyaluronic acid.. Genes Nutr 2014 Sep;9(5):417.
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