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Home / Equine Research Bank / BMC Musculoskelet Disord / Gene expression markers of tendon fibroblasts in normal and ...
BMC musculoskeletal disorders2009; 10; 27; doi: 10.1186/1471-2474-10-27

Gene expression markers of tendon fibroblasts in normal and diseased tissue compared to monolayer and three dimensional culture systems.

Abstract: There is a paucity of data regarding molecular markers that identify the phenotype of the tendon cell. This study aims to quantify gene expression markers that distinguish between tendon fibroblasts and other mesenchymal cells which may be used to investigate tenogenesis. Methods: Expression levels for 12 genes representative of musculoskeletal tissues, including the proposed tendon progenitor marker scleraxis, relative to validated reference genes, were evaluated in matched samples of equine tendon (harvested from the superficial digital flexor tendon), cartilage and bone using quantitative PCR (qPCR). Expression levels of genes associated with tendon phenotype were then evaluated in healthy, including developmental, and diseased equine tendon tissue and in tendon fibroblasts maintained in both monolayer culture and in three dimensional (3D) collagen gels. Results: Significantly increased expression of scleraxis was found in tendon compared with bone (P = 0.002) but not compared to cartilage. High levels of COL1A2 and scleraxis and low levels of tenascin-C were found to be most representative of adult tensional tendon phenotype. While, relative expression of scleraxis in developing mid-gestational tendon or in acute or chronically diseased tendon did not differ significantly from normal adult tendon, tenascin-C message was significantly upregulated in acutely injured equine tendon (P = 0.001). Relative scleraxis gene expression levels in tendon cell monolayer and 3D cultures were significantly lower than in normal adult tendon (P = 0.002, P = 0.02 respectively). Conclusions: The findings of this study indicate that high expression of both COL1A2 and scleraxis, and low expression of tenascin-C is representative of a tensional tendon phenotype. The in vitro culture methods used in these experiments however, may not recapitulate the phenotype of normal tensional tendon fibroblasts in tissues as evidenced by gene expression.
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Publication Date: 2009-02-26 PubMed ID: 19245707PubMed Central: PMC2651848DOI: 10.1186/1471-2474-10-27Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on exploring gene expression markers that differentiate tendon fibroblasts from other similar cells. The study conducted experiments to analyze the expression levels of certain genes in samples procured from equine tissues. The findings suggest high expression of COL1A2 and scleraxis, and low expression of tenascin-C indicate a tensional tendon phenotype. However, in vitro culture methods used in the study may not fully replicate the actual behavior of these cells in tissues.

Objective of the Study

  • The main goal of this study was to identify and quantify gene expression markers that distinguish tendon fibroblasts from other mesenchymal cells.
  • By doing so, the researchers hoped to contribute more information that could be used to study tenogenesis, a term describing the formation and development of tendons.

Methods Adopted

  • Expression levels of 12 genes representative of musculoskeletal tissues were evaluated. Among these was the proposed tendon progenitor marker, scleraxis.
  • The evaluation was conducted on samples of equine tendon, cartilage, and bone, using a method called quantitative PCR (qPCR).
  • The study then investigated gene expression levels associated with tendon phenotype in healthy, developing, and diseased equine tendon tissue.
  • Experiments also included studying tendon fibroblasts maintained in both monolayer culture and in three-dimensional (3D) collagen gels.

Study Results

  • The analysis revealed a significant increase in scleraxis expression in tendon compared to bone, but no substantial difference was found when compared to cartilage.
  • In terms of representing an adult tendon phenotype, high levels of COL1A2 and scleraxis and low levels of tenascin-C were found to be the most indicative.
  • Furthermore, the expression of the scleraxis gene in developing or diseased tendons did not significantly differ from its expression in normal tendons.
  • Contrastingly, tenascin-C expression was significantly higher in tendons with acute injuries.
  • Relative to normal adult tendon, scleraxis gene expression levels in tendon cell monolayer and 3D cultures were significantly lower.

Study Conclusions

  • The study concludes that a high expression of COL1A2 and scleraxis, coupled with a low expression of tenascin-C, is representative of a tensional tendon phenotype.
  • However, the researchers also noted that the in vitro culture methods they employed may not accurately represent the phenotype of normal tensional tendon fibroblasts in tissue, as indicated by disparities in the gene expression.

Cite This Article

APA
Taylor SE, Vaughan-Thomas A, Clements DN, Pinchbeck G, Macrory LC, Smith RK, Clegg PD. (2009). Gene expression markers of tendon fibroblasts in normal and diseased tissue compared to monolayer and three dimensional culture systems. BMC Musculoskelet Disord, 10, 27. https://doi.org/10.1186/1471-2474-10-27

Publication

BMC musculoskeletal disorders
ISSN: 1471-2474
NlmUniqueID: 100968565
Country: England
Language: English
Volume: 10
Pages: 27

Researcher Affiliations

Taylor, Sarah E
  • Department of Veterinary Clinical Science, University of Liverpool, Neston, South Wirral, UK. Sarah.Taylor@liv.ac.uk
Vaughan-Thomas, Anne
    Clements, Dylan N
      Pinchbeck, Gina
        Macrory, Lisa C
          Smith, Roger K W
            Clegg, Peter D

              MeSH Terms

              • Animals
              • Basic Helix-Loop-Helix Transcription Factors / genetics
              • Basic Helix-Loop-Helix Transcription Factors / metabolism
              • Cartilage, Articular / metabolism
              • Cartilage, Articular / pathology
              • Cell Culture Techniques / methods
              • Collagen / metabolism
              • Fibroblasts / metabolism
              • Fibroblasts / pathology
              • Gels
              • Gene Expression
              • Genetic Markers
              • Horses
              • Metacarpal Bones / metabolism
              • Metacarpal Bones / pathology
              • Tendinopathy / metabolism
              • Tendinopathy / pathology
              • Tendons / metabolism
              • Tendons / pathology

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