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Home / Equine Research Bank / Genes (Basel) / Genome-Wide Association Analyses of Equine Metabolic Syndrom...
Genes2019; 10(11); 893; doi: 10.3390/genes10110893

Genome-Wide Association Analyses of Equine Metabolic Syndrome Phenotypes in Welsh Ponies and Morgan Horses.

Abstract: Equine metabolic syndrome (EMS) is a complex trait for which few genetic studies have been published. Our study objectives were to perform within breed genome-wide association analyses (GWA) to identify associated loci in two high-risk breeds, coupled with meta-analysis to identify shared and unique loci between breeds. GWA for 12 EMS traits identified 303 and 142 associated genomic regions in 264 Welsh ponies and 286 Morgan horses, respectively. Meta-analysis demonstrated that 65 GWA regions were shared across breeds. Region boundaries were defined based on a fixed-size or the breakdown of linkage disequilibrium, and prioritized if they were: shared between breeds or across traits (high priority), identified in a single GWA cohort (medium priority), or shared across traits with no SNPs reaching genome-wide significance (low priority), resulting in 56 high, 26 medium, and seven low priority regions including 1853 candidate genes in the Welsh ponies; and 39 high, eight medium, and nine low priority regions including 1167 candidate genes in the Morgans. The prioritized regions contained protein-coding genes which were functionally enriched for pathways associated with inflammation, glucose metabolism, or lipid metabolism. These data demonstrate that EMS is a polygenic trait with breed-specific risk alleles as well as those shared across breeds.
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Publication Date: 2019-11-06 PubMed ID: 31698676PubMed Central: PMC6895807DOI: 10.3390/genes10110893Google Scholar: Lookup
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  • Journal Article
  • Meta-Analysis
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a genetic study that uncovers the potential genetic factors underlying the susceptibility to equine metabolic syndrome (EMS) in Welsh ponies and Morgan horses – two high-risk breeds.

Research Goals and Approaches

  • The main aim of the study was to carry out within-breed genome-wide association analyses (GWA) to pinpoint the genetic loci linked with EMS within Welsh ponies and Morgan horses.
  • The researchers also aimed to perform a meta-analysis which combines the results of each breed’s GWA to reveal both shared and breed-specific loci linked with EMS.

Methodology and Findings

  • The GWA studies for 12 EMS traits were conducted in groups of 264 Welsh ponies and 286 Morgan horses, revealing 303 and 142 associated genomic regions respectively.
  • From a meta-analysis, 65 of these identified regions were found to be shared between these two breeds.

Region Prioritization and Candidate Genes

  • The researchers determined the boundaries of the associated regions based on either a fixed size or the breakdown of linkage disequilibrium – a measure of the non-random association of alleles at two or more loci.
  • The identified regions were prioritized based on whether they were shared between breeds/traits (high priority), found in a single GWA cohort (medium priority), or shared across traits without any SNPs reaching genome-wide significance (low priority).
  • Based on this, there were 56 high, 26 medium, and seven low priority regions in Welsh ponies, and 39 high, eight medium, and nine low priority regions in Morgan horses.
  • These prioritized regions contained 1853 and 1167 candidate genes in Welsh ponies and Morgans respectively.

Conclusion

  • The genes found in the prioritized regions were significantly related to inflammation, glucose metabolism, or lipid metabolism. These are all pathways considered important in EMS pathogenesis.
  • The study concluded that EMS is a polygenic trait, implicating multiple genes in its manifestation. It was also found that there are both breed-specific risk alleles associated with EMS as well as those that are shared between breeds

Cite This Article

APA
Norton E, Schultz N, Geor R, McFarlane D, Mickelson J, McCue M. (2019). Genome-Wide Association Analyses of Equine Metabolic Syndrome Phenotypes in Welsh Ponies and Morgan Horses. Genes (Basel), 10(11), 893. https://doi.org/10.3390/genes10110893

Publication

Genes
ISSN: 2073-4425
NlmUniqueID: 101551097
Country: Switzerland
Language: English
Volume: 10
Issue: 11
PII: 893

Researcher Affiliations

Norton, Elaine
  • Veterinary Population Medicine Department, University of Minnesota, St. Paul, MN 55108, USA.
Schultz, Nichol
  • Veterinary Population Medicine Department, University of Minnesota, St. Paul, MN 55108, USA.
Geor, Ray
  • College of Sciences, Massey University, Palmerston North 4442, New Zealand.
McFarlane, Dianne
  • Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
Mickelson, James
  • Veterinary Biomedical Sciences Department, University of Minnesota; St. Paul, MN 55108, USA.
McCue, Molly
  • Veterinary Population Medicine Department, University of Minnesota, St. Paul, MN 55108, USA.

MeSH Terms

  • Alleles
  • Animals
  • Female
  • Genetic Predisposition to Disease / genetics
  • Genome-Wide Association Study / methods
  • Genome-Wide Association Study / veterinary
  • Genomics / methods
  • Genotype
  • Horses / genetics
  • Insulin / metabolism
  • Linkage Disequilibrium / genetics
  • Male
  • Metabolic Syndrome / genetics
  • Metabolic Syndrome / veterinary
  • Phenotype
  • Polymorphism, Single Nucleotide / genetics
  • Quantitative Trait Loci / genetics
  • Risk Factors

Grant Funding

  • T32OD010996 / Foundation for the National Institutes of Health

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 5 times.
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