HomeEquine Research BankOsteoarthritis CartilageGlucosamine HCl reduces equine articular cartilage degradati...

Osteoarthritis and cartilage2000; 8(4); 258-265; doi: 10.1053/joca.1999.0299

Glucosamine HCl reduces equine articular cartilage degradation in explant culture.

Fenton, JI
·
Chlebek-Brown, KA
·
Peters, TL
·
Caron, JP
·
Orth, MW

Abstract: Objective To determine whether glucosamine inhibits experimentally induced degradation of equine articular cartilage explants. Methods Articular cartilage was obtained from the antebrachio-carpal and middle joints of horses (2-8 years old) killed for reasons unrelated to lameness. Cartilage discs were harvested from the weight-bearing region of the articular surface and cultured. Media were exchanged daily and the recovered media stored at 4 degrees C. Explants were maintained in basal media 2 days prior to the start of four treatment days. On days 1-4 lipopolysaccharide (LPS, 10 microg/ml) or recombinant human interleukin-1 (rhIL-1, 50 ng/ml) were added to induce cartilage degradation. To test the potential protective effects of glucosamine, the compound was added in three concentrations (0.25, 2.5, or 25 mg/ml) and treatments were performed in triplicate. Controls included wells without LPS, rhIL-1beta, or glucosamine. Nitric oxide, proteoglycan and matrix metalloproteinases (MMP) released into conditioned media and tissue proteoglycan synthesis were measured as indicators of cartilage metabolism. Results Maximal nitric oxide production, proteoglycan release, and MMP activity were detected 1 day after the addition of LPS or rhIL-1beta to the media. The addition of 25 mg/ml of glucosamine prevented the increase in nitric oxide production, proteoglycan release and MMP activity induced by LPS or rhIL-1. Conclusions These data indicate that glucosamine can prevent experimentally induced cartilage degradation in vitro.

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Publication Date: 2000-07-25 PubMed ID: 10903879DOI: 10.1053/joca.1999.0299Google Scholar: Lookup

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MeSH
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Journal Article
Research Support
Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper investigates whether glucosamine can inhibit the degradation of horse cartilage in an experimental setting. The results show that glucosamine effectively prevented increases in indicators of cartilage metabolism, hinting at glucosamine’s potential in tackling cartilage degradation issues.

Methodology

The researchers obtained articular cartilage from the antebrachio-carpal and middle joints of horses aged 2-8 years, which were killed for reasons unrelated to lameness.
Cartilage discs were collected from the weight-bearing region of the articular surface and cultured. The media for these cultures were exchanged daily, with the recovered media stored at 4 degrees C.
The explants were kept in basal media for two days before beginning a four-day treatment process. During the treatment, lipopolysaccharide (LPS, 10 microg/ml) or recombinant human interleukin-1 (rhIL-1, 50 ng/ml) was added to induce cartilage degradation.
To gauge the potential protective effects of glucosamine, it was added in three concentrations (0.25, 2.5, or 25 mg/ml). These treatments were conducted in triplicate.
Controls were included that had neither LPS, rhIL-1beta, nor glucosamine.

Measurements

Nitric oxide, proteoglycan, and matrix metalloproteinases (MMP) released into the conditioned media were measured. These substances serve as indicators of cartilage metabolism.
The synthesis of tissue proteoglycan was also observed and measured.

Results

Maximal nitric oxide production, proteoglycan release, and MMP activity were observed one day following the addition of LPS or rhIL-1beta to the media.
The addition of 25 mg/ml of glucosamine prevented the rise in nitric oxide production, proteoglycan release, and MMP activity brought on by LPS or rhIL-1.

Conclusions

These findings indicate that glucosamine could potentially inhibit cartilage degradation induced in a lab setting.

Cite This Article

APA

Fenton JI, Chlebek-Brown KA, Peters TL, Caron JP, Orth MW. (2000). Glucosamine HCl reduces equine articular cartilage degradation in explant culture. Osteoarthritis Cartilage, 8(4), 258-265. https://doi.org/10.1053/joca.1999.0299

Publication

Osteoarthritis and cartilage

ISSN: 1063-4584

NlmUniqueID: 9305697

Country: England

Language: English

Volume: 8

Issue: 4

Pages: 258-265

Researcher Affiliations

Fenton, J I

Department of Animal Science, Michigan State University, East Lansing, Michigan 48824, USA.

Chlebek-Brown, K A

Peters, T L

Caron, J P

Orth, M W

MeSH Terms

Animals
Cartilage, Articular / drug effects
Cartilage, Articular / metabolism
Cells, Cultured
Glucosamine / therapeutic use
Horse Diseases / drug therapy
Horse Diseases / metabolism
Horses
Humans
Interleukin-1
Lipopolysaccharides
Matrix Metalloproteinases / metabolism
Nitric Oxide / biosynthesis
Osteoarthritis / drug therapy
Osteoarthritis / metabolism
Osteoarthritis / veterinary
Proteoglycans / biosynthesis

Citations

This article has been cited 24 times.

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