Group C Streptococcus dysgalactiae subsp. equisimilis in south-east Brazil: genetic diversity, resistance profile and the first report of human and equine isolates belonging to the same multilocus sequence typing lineage.
Abstract: Streptococcus dysgalactiae subsp. equisimilis (SDSE) isolates are the most common group C streptococci in humans and reports of invasive infections associated with SDSE have been increasing. Molecular epidemiology studies are an important strategy to trace the emergence and spread of possible well-fit bacterial pathogens of humans and animals. In this work, we analysed the antimicrobial and clonal profiles of 115 SDSE infection and colonization isolates of human and equine origin. PFGE revealed the spread of two main clusters: clone A (57.4%) and clone A (26.1%). Remarkably, two isolates from clone B obtained from human colonization cases displayed identical PFGE patterns to those of three equine infection isolates. In addition, multilocus sequence typing allocated these isolates to ST129 (CC31). All of the SDSE isolates were susceptible to penicillin, vancomycin, gentamicin, levofloxacin and chloramphenicol. Tetracycline and erythromycin resistance rates were 65.2 and 13.9% respectively. Nevertheless, none of the isolates displaying sporadic PFGE patterns showed erythromycin resistance. The majority of erythromycin-resistant isolates from clone A had inducible resistance to macrolides, lincosamines and streptogramins B (iMLSB phenotype), which is associated with the presence of the ermA gene, whereas the resistant isolates from clone B showed the M phenotype, associated with the mefA gene. In conclusion, the data indicated that the analysed collection of SDSE isolates displayed a clonal structure and that the isolates found in human colonization cases could also be involved in equine infections.
© 2015 The Authors.
Publication Date: 2015-03-09 PubMed ID: 25752850DOI: 10.1099/jmm.0.000052Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article focuses on the genetic diversity and resistance profile of a particular type of group C Streptococcus found in south-east Brazil. The study explores the molecular epidemiology, the spread of infection, and the resistance capacities of SDSE (Streptococcus dysgalactiae subsp. equisimilis) found in humans and equines. Researchers notably found that the same genetic strain of the bacteria occurred in both humans and equines, which underscores an epidemiological link between these two populations.
Understanding Molecular Epidemiology
- The scientists performed an in-depth study of molecular epidemiology to understand and trace the emergence and spread of bacterial pathogens in human and animal populations.
- The specific focus was on the SDSE bacteria, which is the most common group C streptococci in humans and equine species.
- The researchers analyzed the antimicrobial and clonal profiles of 115 SDSE isolates that had resulted in both infection and colonization.
Results of PFGE Analysis
- PFGE (Pulsed-field gel electrophoresis) revealed the spread of two main clusters, clone A and clone B.
- Interestingly, two isolates from clone B, obtained from human colonization cases, showed identical PFGE patterns to isolates from equine infections.
- This suggests a clear epidemiological link between human and equine infections due to the identical genetic profile of the SDSE isolates.
Findings on Antibiotic Resistance
- All analyzed SDSE isolates were found to be susceptible to penicillin, vancomycin, gentamicin, levofloxacin, and chloramphenicol.
- Resistance rates to tetracycline and erythromycin were found to be 65.2% and 13.9% respectively.
- The research uncovered that the erythromycin-resistant isolates from clone A had inducible resistance to macrolides, lincosamines, and streptogramins B (iMLSB phenotype), associated with the presence of the ermA gene.
- Resistant isolates from clone B exhibited the M phenotype, associated with the mefA gene.
- The study therefore highlights the potential for antibiotic resistance in SDSE isolates, and the different resistance paths of different clones.
Conclusions of the Study
- The study found that the analyzed collection of SDSE isolates displayed a clonal structure, this means they share identical DNA sequences, signifying a common origin or replication.
- SDSE isolates found in human colonization cases could also be involved in equine infections, highlighting the potential for cross-species transmission of SDSE.
- This research allows for a better understanding of the epidemiology of SDSE and how it spreads between human and animal populations.
Cite This Article
APA
Silva LG, Genteluci GL, Corrêa de Mattos M, Glatthardt T, Sá Figueiredo AM, Ferreira-Carvalho BT.
(2015).
Group C Streptococcus dysgalactiae subsp. equisimilis in south-east Brazil: genetic diversity, resistance profile and the first report of human and equine isolates belonging to the same multilocus sequence typing lineage.
J Med Microbiol, 64(Pt 5), 551-558.
https://doi.org/10.1099/jmm.0.000052 Publication
Researcher Affiliations
- Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, RJ, Brazil.
- Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, RJ, Brazil.
- Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, RJ, Brazil.
- Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, RJ, Brazil.
- Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, RJ, Brazil.
- Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, RJ, Brazil.
MeSH Terms
- Animals
- Anti-Bacterial Agents / pharmacology
- Brazil / epidemiology
- Cluster Analysis
- Drug Resistance, Bacterial
- Electrophoresis, Gel, Pulsed-Field
- Genetic Variation
- Genotype
- Horses
- Humans
- Microbial Sensitivity Tests
- Molecular Epidemiology
- Multilocus Sequence Typing
- Streptococcal Infections / epidemiology
- Streptococcal Infections / veterinary
- Streptococcus / classification
- Streptococcus / genetics
- Streptococcus / isolation & purification
Citations
This article has been cited 10 times.- Berbel D, González-Díaz A, López de Egea G, Càmara J, Ardanuy C. An Overview of Macrolide Resistance in Streptococci: Prevalence, Mobile Elements and Dynamics.. Microorganisms 2022 Nov 23;10(12).
- Stefańska I, Kwiecień E, Kizerwetter-Świda M, Chrobak-Chmiel D, Rzewuska M. Tetracycline, Macrolide and Lincosamide Resistance in Streptococcus canis Strains from Companion Animals and Its Genetic Determinants.. Antibiotics (Basel) 2022 Jul 31;11(8).
- Barros RR. Antimicrobial Resistance among Beta-Hemolytic Streptococcus in Brazil: An Overview.. Antibiotics (Basel) 2021 Aug 12;10(8).
- Ding YX, Wu Q, Guo Y, Li M, Li PF, Ma Y, Liu WC. Effects of in vitro-induced drug resistance on the virulence of Streptococcus.. Vet Med Sci 2021 May;7(3):935-943.
- Alves-Barroco C, Rivas-García L, Fernandes AR, Baptista PV. Tackling Multidrug Resistance in Streptococci - From Novel Biotherapeutic Strategies to Nanomedicines.. Front Microbiol 2020;11:579916.
- Oh SI, Kim JW, Kim J, So B, Kim B, Kim HY. Molecular subtyping and antimicrobial susceptibility of Streptococcus dysgalactiae subspecies equisimilis isolates from clinically diseased pigs.. J Vet Sci 2020 Jul;21(4):e57.
- Oh SI, Kim JW, Jung JY, Chae M, Lee YR, Kim JH, So B, Kim HY. Pathologic and molecular characterization of Streptococcus dysgalactiae subsp. equisimilis infection in neonatal piglets.. J Vet Sci 2018 Mar 31;19(2):313-317.
- Kłos M, Wójkowska-Mach J. Pathogenicity of Virulent Species of Group C Streptococci in Human.. Can J Infect Dis Med Microbiol 2017;2017:9509604.
- Ciszewski M, Szewczyk EM. Potential Factors Enabling Human Body Colonization by Animal Streptococcus dysgalactiae subsp. equisimilis Strains.. Curr Microbiol 2017 May;74(5):650-654.
- Pinho MD, Erol E, Ribeiro-Gonçalves B, Mendes CI, Carriço JA, Matos SC, Preziuso S, Luebke-Becker A, Wieler LH, Melo-Cristino J, Ramirez M. Beta-hemolytic Streptococcus dysgalactiae strains isolated from horses are a genetically distinct population within the Streptococcus dysgalactiae taxon.. Sci Rep 2016 Aug 17;6:31736.
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