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Home / Equine Research Bank / Front Physiol / Guanylin, Uroguanylin and Guanylate Cyclase-C Are Expressed ...
Frontiers in physiology2019; 10; 1237; doi: 10.3389/fphys.2019.01237

Guanylin, Uroguanylin and Guanylate Cyclase-C Are Expressed in the Gastrointestinal Tract of Horses.

Abstract: Guanylate cyclase-C (GC-C) is a multifunctional receptor encoded by the gene, representing an attractive target for therapy in several gastrointestinal diseases in humans. Little is known about this system in horses. We investigated for the first time the gene expression of guanylin, uroguanylin and GC-C receptors in different horse's gastrointestinal tracts. Tissue samples from stomach, duodenum, jejunum, ileum, head and body of cecum, left and right dorsal colon, left and right ventral colon, pelvic flexure, transverse colon, descending colon and rectum were collected from adult horses within 1 h . For each sample, total RNA was extracted from 100 mg of ground tissue, and qRT-PCR performed on , and transcripts on a CFX96 Touch instrument. Data analysis was carried out with Bio-Rad CFX Manager software, and genes of interest normalized relative to the abundance of the two reference genes (). Additionally, the protein expression levels of GC-C receptor were analyzed through western blotting. A common pattern of expression throughout the gastrointestinal lumen for all three investigated transcripts was found. The expression of , and genes was higher in jejunum, ileum, descending colon and rectum. The levels of expression of GC-C protein confirmed these data. The findings of this study might open new scenarios for the therapeutic approach to enteric diseases of horse using selective agonists of GC-C.
Copyright © 2019 Cappelli, Gialletti, Tesei, Bassotti, Fettucciari, Capomaccio, Bonfili, Cuccioloni, Eleuteri, Spaterna and Laus.
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Publication Date: 2019-09-27 PubMed ID: 31611814PubMed Central: PMC6776823DOI: 10.3389/fphys.2019.01237Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper details the study of gene expression of guanylin, uroguanylin, and Guanylate cyclase-C (GC-C) receptors, three components involved in gastrointestinal diseases, in different areas of the horse’s gastrointestinal tract. This is the first time such a study has been conducted and it opens the potential for new therapies for equine enteric diseases.

Studying GC-C and Its Ligands in Horses

  • Guanylate cyclase-C (GC-C) is a receptor that can perform multiple functions and is encoded by the GUCY2C gene. It is particularly interesting for scientists because it can be a target for therapy in multiple gastrointestinal diseases in human beings.
  • In spite of its significance, there has been little research on this receptor in horses, which is what the authors of this paper set out to investigate. They specifically look at the gene expression of guanylin, uroguanylin, which are ligands, and GC-C receptors in the gastrointestinal tract of horses.

Methodology of the Study

  • From adult horses the researchers collected tissue samples, within one hour of extraction, from various parts of the gastrointestinal tract, including the stomach, duodenum, and different parts of the colon and rectum.
  • Using 100mg of ground tissue from each sample, they extracted total RNA. Subsequently, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to analyze guanylin, uroguanylin, and GC-C receptor transcripts, utilizing a CFX96 Touch instrument.
  • The Bio-Rad CFX Manager software was utilized to conduct data analysis. The genes of interest were examined relative to the abundance of two reference genes.
  • Additionally, the protein expression levels of the GC-C receptor were examined through western blotting, another analytical technique.

Findings of the Study and Future Applications

  • The researchers found a similar pattern of expression throughout the entire gastrointestinal lumen for all three transcripts they examined.
  • The expression of guanylin, uroguanylin, and GUCY2C genes was shown to be higher in the jejunum, ileum, descending colon, and rectum. The protein expression levels of GC-C confirmed these findings.
  • These insights could potentially open new avenues for therapy for enteric diseases in horses. This study’s findings suggest that these diseases could be treated with selective agonists of GC-C.

Cite This Article

APA
Cappelli K, Gialletti R, Tesei B, Bassotti G, Fettucciari K, Capomaccio S, Bonfili L, Cuccioloni M, Eleuteri AM, Spaterna A, Laus F. (2019). Guanylin, Uroguanylin and Guanylate Cyclase-C Are Expressed in the Gastrointestinal Tract of Horses. Front Physiol, 10, 1237. https://doi.org/10.3389/fphys.2019.01237

Publication

Frontiers in physiology
ISSN: 1664-042X
NlmUniqueID: 101549006
Country: Switzerland
Language: English
Volume: 10
Pages: 1237
PII: 1237

Researcher Affiliations

Cappelli, Katia
  • Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
Gialletti, Rodolfo
  • Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
Tesei, Beniamino
  • School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Bassotti, Gabrio
  • Department of Medicine, School of Medicine, University of Perugia, Perugia, Italy.
Fettucciari, Katia
  • Department of Experimental Medicine, School of Medicine, University of Perugia, Perugia, Italy.
Capomaccio, Stefano
  • Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
Bonfili, Laura
  • School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Cuccioloni, Massimiliano
  • School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Eleuteri, Anna Maria
  • School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Spaterna, Andrea
  • School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Laus, Fulvio
  • School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.

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Citations

This article has been cited 1 times.
  1. Pandey KN. Guanylyl cyclase/natriuretic peptide receptor-A: Identification, molecular characterization, and physiological genomics.. Front Mol Neurosci 2022;15:1076799.
    doi: 10.3389/fnmol.2022.1076799pubmed: 36683859google scholar: lookup
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