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PloS one2024; 19(6); e0305031; doi: 10.1371/journal.pone.0305031

Heart rate and heart rate variability in horses undergoing hot and cold shoeing.

Abstract: Heart rate variability (HRV) is a frequently used indicator of autonomic responses to various stimuli in horses. This study aimed to investigate HRV variables in horses undergoing cold (n = 25) or hot (n = 26) shoeing. Multiple HRV variables were measured and compared between horses undergoing cold and hot shoeing, including the time domain, frequency domain, and nonlinear variables pre-shoeing, during shoeing, and at 30-minute intervals for 120 minutes post-shoeing. The shoeing method interacted with time to change the HRV variables standard deviation of RR intervals (SDNN), root mean square of successive RR interval differences (RMSSD), very-low-frequency band, low-frequency band (LF), the LF to high-frequency band ratio, respiratory rate, total power, standard deviation perpendicular to the line of identity (SD1), and standard deviation along the line of identity (SD2). SDNN, RMSSD, and total power only increased 30 minutes after hot shoeing (all p < 0.05). Triangular interpolation of normal-to-normal intervals (TINN) and the HRV triangular index increased during and up to 120 minutes after hot shoeing (p < 0.05-0.001). TINN increased only during cold shoeing (p < 0.05). LF increased 30 and 60 minutes after hot shoeing (both p < 0.05). SD1 and SD2 also increased 30 minutes after hot shoeing (both p < 0.05). SDNN, TINN, HRV triangular index, LF, total power, and SD2 were higher in hot-shoed than cold-shoed horses throughout the 120 minutes post-shoeing. Differences in HRV were found, indicating increased sympathovagal activity in hot shoed horses compared to cold shoed horses.
Publication Date: 2024-06-06 PubMed ID: 38843254DOI: 10.1371/journal.pone.0305031Google Scholar: Lookup
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Summary

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This research article explores the impact of two different shoeing methods (cold and hot) on the heart rate variability (HRV) of horses, an indicator of autonomic responses. Notable differences in various HRV components were observed, indicating that hot shoeing tends to increase the sympathovagal activity in horses more than cold shoeing.

Study Design and Participants

  • The study was designed to compare the HRV variables in horses that underwent either cold or hot shoeing procedures.
  • Two groups of horses were taken, with 25 horses undergoing cold shoeing and 26 horses undergoing hot shoeing.

Measurement and Comparison of HRV Variables

  • The researchers measured multiple HRV variables, which included time, frequency, and nonlinear variables, at various points – before, during, and after shoeing at 30-minute intervals up to 120 minutes.
  • These variables were then compared between the horses that underwent cold and hot shoeing.

Results and Observations

  • The study showed that the shoeing method significantly influenced various HRV variables over time, such as the standard deviation of RR intervals (SDNN), root mean square of successive RR interval differences (RMSSD), low-frequency band (LF), total power, and others.
  • Hot shoeing particularly increased SDNN, RMSSD, and total power after 30 minutes and also increased LF, SD1 and SD2 after 30-60 minutes.
  • Triangular interpolation of normal-to-normal intervals (TINN) and the HRV triangular index notably increased during the hot shoeing procedure and up to 120 minutes afterward while TINN increased only during cold shoeing.
  • The study also found that SDNN, TINN, HRV triangular index, LF, total power, and SD2 were higher in hot-shoed horses than cold-shoed horses throughout the 120 minutes post-shoeing.
  • This indicated a significantly increased sympathovagal activity in horses that underwent hot shoeing compared to those that underwent cold shoeing.

Implications and Conclusion

  • The study showed distinct differences in HRV variables between hot and cold shoeing methods, indicating that the type of shoeing has a direct impact on the physiological responses of horses.
  • Given the evident increase in sympathovagal activity in horses subjected to hot shoeing, it becomes crucial to understand the implications of this on the overall health of the horses and adapt practices accordingly.

Cite This Article

APA
Huangsaksri O, Wonghanchao T, Sanigavatee K, Poochipakorn C, Chanda M. (2024). Heart rate and heart rate variability in horses undergoing hot and cold shoeing. PLoS One, 19(6), e0305031. https://doi.org/10.1371/journal.pone.0305031

Publication

ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 19
Issue: 6
Pages: e0305031

Researcher Affiliations

Huangsaksri, Onjira
  • Veterinary Clinical Study Programme, Graduate School, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, Thailand.
  • Faculty of Veterinary Medicine, Department of Large Animal and Wildlife Clinical Science, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, Thailand.
Wonghanchao, Thita
  • Veterinary Clinical Study Programme, Graduate School, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, Thailand.
  • Faculty of Veterinary Medicine, Department of Large Animal and Wildlife Clinical Science, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, Thailand.
Sanigavatee, Kanokpan
  • Veterinary Clinical Study Programme, Graduate School, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, Thailand.
  • Faculty of Veterinary Medicine, Department of Large Animal and Wildlife Clinical Science, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, Thailand.
Poochipakorn, Chanoknun
  • Veterinary Clinical Study Programme, Graduate School, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, Thailand.
  • Faculty of Veterinary Medicine, Department of Large Animal and Wildlife Clinical Science, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, Thailand.
Chanda, Metha
  • Faculty of Veterinary Medicine, Department of Large Animal and Wildlife Clinical Science, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, Thailand.
  • Thailand Equestrian Federation, Sports Authority of Thailand, Bangkok, Thailand.

MeSH Terms

  • Animals
  • Horses / physiology
  • Heart Rate / physiology
  • Male
  • Cold Temperature
  • Hot Temperature
  • Female
  • Respiratory Rate / physiology
  • Shoes

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citations

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