High-throughput analysis of horse sperms’ 3D swimming patterns using computational on-chip imaging.
Abstract: Using a high-throughput optical tracking technique that is based on partially-coherent digital in-line holography, here we report a detailed analysis of the statistical behavior of horse sperms' three-dimensional (3D) swimming dynamics. This dual-color and dual-angle lensfree imaging platform enables us to track individual 3D trajectories of ∼1000 horse sperms at sub-micron level within a sample volume of ∼9μL at a frame rate of 143 frames per second (FPS) and collect thousands of sperm trajectories within a few hours for statistical analysis of their 3D dynamics. Using this high-throughput imaging platform, we recorded >17,000 horse sperm trajectories that can be grouped into six major categories: irregular, linear, planar, helical, ribbon, and hyperactivated, where the hyperactivated swimming patterns can be further divided into four sub-categories, namely hyper-progressive, hyper-planar, hyper-ribbon, and star-spin. The large spatio-temporal statistics that we collected with this 3D tracking platform revealed that irregular, planar, and ribbon trajectories are the dominant 3D swimming patterns observed in horse sperms, which altogether account for >97% of the trajectories that we imaged in plasma-free semen extender medium. Through our experiments we also found out that horse seminal plasma in general increases sperms' straightness in their 3D trajectories, enhancing the relative percentage of linear swimming patterns and suppressing planar swimming patterns, while barely affecting the overall percentage of ribbon patterns.
Copyright © 2016 Elsevier B.V. All rights reserved.
Publication Date: 2016-01-07 PubMed ID: 26826909DOI: 10.1016/j.anireprosci.2015.12.012Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article investigates the three-dimensional movement patterns (dynamics) of horse sperms using a high-output lensless imaging platform based on partially-coherent digital in-line holography.
High-throughput Imaging Technique
- The researchers utilized an optical tracking technique that builds on partially-coherent digital in-line holography. This provided them with a dual-color and dual-angle lensless imaging platform.
- This platform allowed for tracking individual 3D movement paths of about 1000 horse sperms. The tracking was able to be conducted at a sub-micron level within a 9-microliter sample volume.
- The recording speed was 143 frames per second, which allowed for collecting thousands of sperm trajectories within a few hours for detailed statistical analysis of the 3D swimming patterns.
Data Collection and Analysis
- Over 17,000 horse sperm trajectories were recorded using the high-throughput imaging platform.
- These trajectories were categorized into six main patterns – irregular, linear, planar, helical, ribbon, and hyperactivated. The hyperactivated swimming patterns were further divided into four sub-categories – hyper-progressive, hyper-planar, hyper-ribbon, and star-spin.
- Analysis of the extensive spatio-temporal data showed that irregular, planar, and ribbon trajectories were the major 3D swimming patterns seen in horse sperms.
- Collectively, these patterns constituted over 97% of the processed trajectories in plasma-free semen extender medium.
The Impact of Horse Seminal Plasma
- The research experiments also observed the effect of horse seminal plasma on the movement patterns of sperms.
- The presence of seminal plasma was found to generally increase the straightness of sperms’ 3D movements. This enhanced the relative percentage of linear swimming patterns and lessened the expression of planar swimming patterns.
- The seminal plasma, however, didn’t significantly affect the overall percentage of ribbon patterns.
Cite This Article
APA
Su TW, Choi I, Feng J, Huang K, Ozcan A.
(2016).
High-throughput analysis of horse sperms’ 3D swimming patterns using computational on-chip imaging.
Anim Reprod Sci, 169, 45-55.
https://doi.org/10.1016/j.anireprosci.2015.12.012 Publication
Researcher Affiliations
- Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA; Bioengineering Department, University of California, Los Angeles, CA 90095, USA.
- Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.
- Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.
- Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.
- Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA; Bioengineering Department, University of California, Los Angeles, CA 90095, USA; California NanoSystems Institute (CNSI), University of California, Los Angeles, CA 90095, USA; Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA. Electronic address: ozcan@ucla.edu.
MeSH Terms
- Animals
- High-Throughput Screening Assays / instrumentation
- High-Throughput Screening Assays / veterinary
- Horses / physiology
- Image Processing, Computer-Assisted
- Lab-On-A-Chip Devices
- Male
- Sperm Motility / physiology
- Spermatozoa / physiology
Citations
This article has been cited 8 times.- Picazo-Bueno JÁ, Sanz M, Granero L, García J, Micó V. Multi-Illumination Single-Holographic-Exposure Lensless Fresnel (MISHELF) Microscopy: Principles and Biomedical Applications. Sensors (Basel) 2023 Jan 28;23(3).
- Mirecki B, Rogalski M, Arcab P, Rogujski P, Stanaszek L, Józwik M, Trusiak M. Low-intensity illumination for lensless digital holographic microscopy with minimized sample interaction. Biomed Opt Express 2022 Nov 1;13(11):5667-5682.
- Wiesehöfer C, Wiesehöfer M, Dankert JT, Chung JJ, von Ostau NE, Singer BB, Wennemuth G. CatSper and its CaM-like Ca(2+) sensor EFCAB9 are necessary for the path chirality of sperm. FASEB J 2022 May;36(5):e22288.
- Daloglu MU, Luo W, Shabbir F, Lin F, Kim K, Lee I, Jiang JQ, Cai WJ, Ramesh V, Yu MY, Ozcan A. Label-free 3D computational imaging of spermatozoon locomotion, head spin and flagellum beating over a large volume. Light Sci Appl 2018;7:17121.
- Daloglu MU, Lin F, Chong B, Chien D, Veli M, Luo W, Ozcan A. 3D imaging of sex-sorted bovine spermatozoon locomotion, head spin and flagellum beating. Sci Rep 2018 Oct 23;8(1):15650.
- Muschol M, Wenders C, Wennemuth G. Four-dimensional analysis by high-speed holographic imaging reveals a chiral memory of sperm flagella. PLoS One 2018;13(6):e0199678.
- Daloglu MU, Ozcan A. Computational imaging of sperm locomotion. Biol Reprod 2017 Aug 1;97(2):182-188.
- Kim J, Lee SJ. Digital in-line holographic microscopy for label-free identification and tracking of biological cells. Mil Med Res 2024 Jun 13;11(1):38.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
