FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Journal of clinical microbiology1982; 16(2); 307-313; doi: 10.1128/jcm.16.2.307-313.1982

Horse erythrocyte glycoprotein-latex reagent that reacts with infectious mononucleosis heterophile antibody.

Abstract: A sialoglycoprotein from horse erythrocytes was isolated in essentially homogeneous form and found to contain the neuraminidase-sensitive determinant of the horse erythrocyte for Paul-Bunnell heterophile antibodies of infectious mononucleosis. This reactivity was retained after covalent coupling of the antigen to latex particles. The latex reagent has greater stability (greater than 3 years) than either fresh or preserved horse erythrocytes. It can be used in a direct slide test; no absorption of the serum is necessary. The new test compared favorably with some standard tests for infectious mononucleosis antibody which use horse erythrocytes. Agreement of the latex test with the absorbed fresh horse cell test was 100%, and agreement with a stabilized horse erythrocyte spot test was 94%. The latex test also agreed 100% with a radioimmunoassay for the detection of heterophile antibody with 125I-labeled horse erythrocyte glycoprotein. The new latex test was compared with a bovine erythrocyte glycoprotein-latex test, and a correlation of 94% was observed. In addition, it was compared with the Wöllner test (enzyme-treated sheep cell-absorbed sheep cell agglutination test) with which it agreed in 92% of samples tested.
Get Full Text
Publication Date: 1982-08-01 PubMed ID: 6896881PubMed Central: PMC272351DOI: 10.1128/jcm.16.2.307-313.1982Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents the findings of a study that tested the use of horse erythrocyte glycoprotein-latex reagent in diagnosing infectious mononucleosis, an infectious disease. The study found this method to be more stable and comparably accurate to other commonly used tests.

Research Methodology

  • The researchers isolated a sialoglycoprotein from horse erythrocytes, which was found to contain the neuraminidase-sensitive determinant of the horse erythrocyte for Paul-Bunnell heterophile antibodies of infectious mononucleosis.
  • This glycoprotein’s reactivity was maintained even after it was covalently coupled with latex particles to form a reagent.
  • The reagent’s stability was tested and found to be greater than three years, superior to either fresh or preserved horse erythrocytes.

Comparison with Existing Tests

  • The latex reagent could be used in a direct slide test and did not require serum absorption. This attribute was compared with existing tests for diagnosing infectious mononucleosis.
  • The new latex test showed 100% agreement with the absorbed fresh horse cell test and 94% agreement with a stabilized horse erythrocyte spot test.
  • The result of the latex test also showed 100% agreement with a radiommunoassay that uses 125I-labeled horse erythrocyte glycoprotein to detect heterophile antibody.
  • Compared with a bovine erythrocyte glycoprotein-latex test there was 94% correlation.
  • Lastly, a comparison was made with the Wöllner test, which uses enzyme-treated sheep cell-absorbed sheep cell agglutination, and it showed an agreement of 92% on samples tested.

Conclusions

  • Overall, the research validates the effective use of the horse erythrocyte glycoprotein-latex reagent in diagnosing infectious mononucleosis.
  • It enables a direct slide test, negating the need for serum absorption and is more stable than alternatives over a long duration.
  • The results also demonstrate a high level of agreement and correlation with existing standard tests, making it a viable alternative for diagnosing the infectious disease.

Cite This Article

APA
Fletcher MA, Caldwell KE, Saez L, Latif Z. (1982). Horse erythrocyte glycoprotein-latex reagent that reacts with infectious mononucleosis heterophile antibody. J Clin Microbiol, 16(2), 307-313. https://doi.org/10.1128/jcm.16.2.307-313.1982

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 16
Issue: 2
Pages: 307-313

Researcher Affiliations

Fletcher, M A
    Caldwell, K E
      Saez, L
        Latif, Z

          MeSH Terms

          • Animals
          • Antibodies, Heterophile / analysis
          • Horses / blood
          • Humans
          • Indicators and Reagents
          • Infectious Mononucleosis / diagnosis
          • Infectious Mononucleosis / immunology
          • Latex Fixation Tests
          • Sialoglycoproteins / blood

          Grant Funding

          • AM16763 / NIADDK NIH HHS

          References

          This article includes 23 references
          1. Miale TD, Barbosa JL, Dellinger CT, Wolfson SL. Positive monospot tests preceding the diagnosis of acute monocytic leukemia in two adolescents.. Med Pediatr Oncol 1978;4(2):111-4.
            pubmed: 275531doi: 10.1002/mpo.2950040205google scholar: lookup
          2. Tsai CM, Zopf DA, Wistar R Jr, Ginsburg V. A human cold agglutinin which binds lacto-N-neotetraose.. J Immunol 1976 Sep;117(3):717-21.
            pubmed: 822101
          3. Beer P. THE HETEROPHILE ANTIBODIES IN INFECTIOUS MONONUCLEOSIS AND AFTER THE INJECTION OF SERUM.. J Clin Invest 1936 Nov;15(6):591-9.
            pubmed: 16694432doi: 10.1172/JCI100811google scholar: lookup
          4. Caldwell KE, Cayer M, Whitney PL, Fletcher MA. Immunochemical studies of infectious mononucleosis--X. Characterization of a glycoprotein from horse erythrocytes which reacts with Paul-Bunnell antibody.. Mol Immunol 1982 Jun;19(6):779-91.
            pubmed: 6810102doi: 10.1016/0161-5890(82)90004-9google scholar: lookup
          5. Fletcher MA, Caldwell KE, Latif ZA, Cayer M, Claflin A. Immunochemical studies of infectious mononucleosis. VIII. A glycoprotein from sheep erythrocytes with sialic acid-dependent receptor properties.. J Immunol 1982 Feb;128(2):976-82.
            pubmed: 7054298
          6. Basson V, Sharp AA. Monospot: a differential slide test for infectious mononucleosis.. J Clin Pathol 1969 May;22(3):324-5.
            pubmed: 5814738doi: 10.1136/jcp.22.3.324google scholar: lookup
          7. Fletcher MA, Woolfolk BJ. Immunochemical studies of infectious mononucleosis. II. Sodium dodecyl sulfate gel electrophoresis of membrane glycoprotein antigens.. Biochim Biophys Acta 1972 Aug 31;278(1):163-74.
            pubmed: 5069590doi: 10.1016/0005-2795(72)90118-3google scholar: lookup
          8. Lee CL, Davidsohn I, Panczyszyn O. Horse agglutinins in infectious mononucleosis. II. The spot test.. Am J Clin Pathol 1968 Jan;49(1):12-8.
            pubmed: 5635266doi: 10.1093/ajcp/49.1.12google scholar: lookup
          9. Fletcher MA, Woolfolk BJ. Immunochemical studies on infectious mononucleosis. I. Isolation and characterization of heterophile antigens from hemoglobin-free stroma.. J Immunol 1971 Sep;107(3):842-53.
            pubmed: 4999098
          10. Evans AS, Niederman JC, Cenabre LC, West B, Richards VA. A prospective evaluation of heterophile and Epstein-Barr virus-specific IgM antibody tests in clinical and subclinical infectious mononucleosis: Specificity and sensitivity of the tests and persistence of antibody.. J Infect Dis 1975 Nov;132(5):546-54.
            pubmed: 171321doi: 10.1093/infdis/132.5.546google scholar: lookup
          11. Henle G, Henle W, Diehl V. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis.. Proc Natl Acad Sci U S A 1968 Jan;59(1):94-101.
            pubmed: 5242134doi: 10.1073/pnas.59.1.94google scholar: lookup
          12. Feorino PM, Dye LA, Humphrey DD. Comparison of diagnostic tests for infectious mononucleosis.. J Am Coll Health Assoc 1971 Feb;19(3):190-3.
            pubmed: 5101085
          13. Lee CL, Davidsohn I, Slaby R. Horse agglutinins in infectious mononucleosis.. Am J Clin Pathol 1968 Jan;49(1):3-11.
            pubmed: 5635272doi: 10.1093/ajcp/49.1.3google scholar: lookup
          14. Horwitz CA, Henle W, Henle G, Penn G, Hoffman N, Ward PC. Persistent falsely positive rapid tests for infectious mononucleosis. Report of five cases with four--six-year follow-up data.. Am J Clin Pathol 1979 Nov;72(5):807-11.
            pubmed: 228546doi: 10.1093/ajcp/72.5.807google scholar: lookup
          15. Fraker PJ, Speck JC Jr. Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril.. Biochem Biophys Res Commun 1978 Feb 28;80(4):849-57.
            pubmed: 637870doi: 10.1016/0006-291x(78)91322-0google scholar: lookup
          16. Dejter-Juszynski M, Harpaz N, Flowers HM, Sharon N. Blood-group ABH-specific macroglycolipids of human erythrocytes: isolation in high yield from a crude membrane glycoprotein fraction.. Eur J Biochem 1978 Feb;83(2):363-73.
            pubmed: 631124doi: 10.1111/j.1432-1033.1978.tb12102.xgoogle scholar: lookup
          17. Hoff G, Bauer S. A new rapid slide test for infectious mononucleosis.. JAMA 1965 Oct 25;194(4):351-3.
            pubmed: 5897418
          18. Fletcher MA, Lo TM, Levey BA, Graves WR. Immunochemical studies of infectious mononucleosis. VI. a radioimmunoassay for the detection of infectious mononucleosis heterophile antibody and antigen.. J Immunol Methods 1977;14(1):51-8.
            pubmed: 299865doi: 10.1016/s0022-1759(97)90021-1google scholar: lookup
          19. Golubjatnikov R, Koehler JE, Inhorn SL. Absorbed heterophile and ox-cell hemolysin test in serodiagnosis of infectious mononucleosis.. Health Lab Sci 1975 Jul;12(3):201-7.
            pubmed: 1236612
          20. Schmitz H, Scherer M. IgM antibodies to Epstein-Barr virus in infectious mononucleosis.. Arch Gesamte Virusforsch 1972;37(4):332-9.
            pubmed: 4339106doi: 10.1007/BF01241456google scholar: lookup
          21. Levey BA, Lo TM, Caldwell KE, Fletcher MA. Latex test for serodiagnosis of infectious mononucleosis.. J Clin Microbiol 1980 Mar;11(3):256-62.
            pubmed: 6247367doi: 10.1128/jcm.11.3.256-262.1980google scholar: lookup
          22. Sadoff L, Goldsmith O. False-positive infectious mononucleosis spot test in pancreatic carcinoma.. JAMA 1971 Nov 22;218(8):1297-8.
            pubmed: 5171178
          23. Wahren B. Diagnosis of infectious mononucleosis by the monospot test.. Am J Clin Pathol 1969 Sep;52(3):303-8.
            pubmed: 4896619doi: 10.1093/ajcp/52.3.303google scholar: lookup

          Citations

          This article has been cited 1 times.
          1. Fletcher MA, Klimas NG, Latif ZA, Caldwell KE. Serodiagnosis of infectious mononucleosis with a bovine erythrocyte glycoprotein. J Clin Microbiol 1983 Sep;18(3):495-9.
            doi: 10.1128/jcm.18.3.495-499.1983pubmed: 6630440google scholar: lookup
          Subscribe to our newsletter
          Join 100,127 horse owners like you who receive our email updates on horse health and nutrition.