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Home / Equine Research Bank / J Clin Microbiol / Horse erythrocyte glycoprotein-latex reagent that reacts wit...
Journal of clinical microbiology1982; 16(2); 307-313; doi: 10.1128/jcm.16.2.307-313.1982

Horse erythrocyte glycoprotein-latex reagent that reacts with infectious mononucleosis heterophile antibody.

Abstract: A sialoglycoprotein from horse erythrocytes was isolated in essentially homogeneous form and found to contain the neuraminidase-sensitive determinant of the horse erythrocyte for Paul-Bunnell heterophile antibodies of infectious mononucleosis. This reactivity was retained after covalent coupling of the antigen to latex particles. The latex reagent has greater stability (greater than 3 years) than either fresh or preserved horse erythrocytes. It can be used in a direct slide test; no absorption of the serum is necessary. The new test compared favorably with some standard tests for infectious mononucleosis antibody which use horse erythrocytes. Agreement of the latex test with the absorbed fresh horse cell test was 100%, and agreement with a stabilized horse erythrocyte spot test was 94%. The latex test also agreed 100% with a radioimmunoassay for the detection of heterophile antibody with 125I-labeled horse erythrocyte glycoprotein. The new latex test was compared with a bovine erythrocyte glycoprotein-latex test, and a correlation of 94% was observed. In addition, it was compared with the Wöllner test (enzyme-treated sheep cell-absorbed sheep cell agglutination test) with which it agreed in 92% of samples tested.
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Publication Date: 1982-08-01 PubMed ID: 6896881PubMed Central: PMC272351DOI: 10.1128/jcm.16.2.307-313.1982Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents the findings of a study that tested the use of horse erythrocyte glycoprotein-latex reagent in diagnosing infectious mononucleosis, an infectious disease. The study found this method to be more stable and comparably accurate to other commonly used tests.

Research Methodology

  • The researchers isolated a sialoglycoprotein from horse erythrocytes, which was found to contain the neuraminidase-sensitive determinant of the horse erythrocyte for Paul-Bunnell heterophile antibodies of infectious mononucleosis.
  • This glycoprotein’s reactivity was maintained even after it was covalently coupled with latex particles to form a reagent.
  • The reagent’s stability was tested and found to be greater than three years, superior to either fresh or preserved horse erythrocytes.

Comparison with Existing Tests

  • The latex reagent could be used in a direct slide test and did not require serum absorption. This attribute was compared with existing tests for diagnosing infectious mononucleosis.
  • The new latex test showed 100% agreement with the absorbed fresh horse cell test and 94% agreement with a stabilized horse erythrocyte spot test.
  • The result of the latex test also showed 100% agreement with a radiommunoassay that uses 125I-labeled horse erythrocyte glycoprotein to detect heterophile antibody.
  • Compared with a bovine erythrocyte glycoprotein-latex test there was 94% correlation.
  • Lastly, a comparison was made with the Wöllner test, which uses enzyme-treated sheep cell-absorbed sheep cell agglutination, and it showed an agreement of 92% on samples tested.

Conclusions

  • Overall, the research validates the effective use of the horse erythrocyte glycoprotein-latex reagent in diagnosing infectious mononucleosis.
  • It enables a direct slide test, negating the need for serum absorption and is more stable than alternatives over a long duration.
  • The results also demonstrate a high level of agreement and correlation with existing standard tests, making it a viable alternative for diagnosing the infectious disease.

Cite This Article

APA
Fletcher MA, Caldwell KE, Saez L, Latif Z. (1982). Horse erythrocyte glycoprotein-latex reagent that reacts with infectious mononucleosis heterophile antibody. J Clin Microbiol, 16(2), 307-313. https://doi.org/10.1128/jcm.16.2.307-313.1982

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 16
Issue: 2
Pages: 307-313

Researcher Affiliations

Fletcher, M A
    Caldwell, K E
      Saez, L
        Latif, Z

          MeSH Terms

          • Animals
          • Antibodies, Heterophile / analysis
          • Horses / blood
          • Humans
          • Indicators and Reagents
          • Infectious Mononucleosis / diagnosis
          • Infectious Mononucleosis / immunology
          • Latex Fixation Tests
          • Sialoglycoproteins / blood

          Grant Funding

          • AM16763 / NIADDK NIH HHS

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          Citations

          This article has been cited 1 times.
          1. Fletcher MA, Klimas NG, Latif ZA, Caldwell KE. Serodiagnosis of infectious mononucleosis with a bovine erythrocyte glycoprotein. J Clin Microbiol 1983 Sep;18(3):495-9.
            doi: 10.1128/jcm.18.3.495-499.1983pubmed: 6630440google scholar: lookup
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