Horse red blood cells frozen with 20% (w/v) glycerol and stored at -150 C for five years.

The research article examines the effect of freezing horse red blood cells (RBC) with a 20% concentration of glycerol and storing them at -150 degrees Celsius for five years. Following this storage period, the research found no negative impacts on the cells’ ability to recover post freeze-thaw or freeze-thaw-wash, or on the cells’ function to transport oxygen.

Research Objectives and Methods

• The study aimed to understand the impact of long-term freezing of horse red blood cells (RBC), with a focus on the RBCs’ ability to recover and function, in terms of oxygen transport, after the freeze-thaw or freeze-thaw-wash procedure.
• The research was conducted on horse RBC that were frozen with a 20% (w/v) glycerol solution and stored at a temperature of -150 degrees Celsius for a period of five years.

Findings and Conclusions

• The primary finding of the research was that five-year storage of horse RBC under the specified conditions (20% glycerol, -150 degrees Celsius) resulted in no adverse effects on the cells’ recovery or oxygen transport function.
• The study also found that the method of adding and removing the glycerol from the horse RBC was a significant consideration in ensuring optimal recovery values post the freeze-thaw or freeze-thaw-wash process.
• Thus, the research demonstrates that horse RBC can be successfully frozen and stored for long periods without hampering the cells’ recovery or oxygen-transport functionality, provided that the procedure of adding and removing glycerol is appropriately handled.